Keep Current with the Latest in Cell Biology Research

PRC2-Indepdendent Actions of H3.3K27M in Embryonic Stem Cell Differentiation

[Nucleic Acids Research] The authors studied the immediate, genome-wide consequences of the H3.3K27M mutation independent of Polycomb Repressor Complex 2 (PRC2) activity. They developed Doxycycline-inducible mouse ESCs carrying a single extra copy of WT-H3.3, H3.3K27M, and H3.3K27L all fused to HA.

Induction of Functional Xeno-Free MSCs from Human iPSCs via a Neural Crest Cell Lineage

[NPJ Regenerative Medicine] The authors induced mesenchymal stem cells from hiPSCs via a neural crest cell lineage under xeno-free conditions and evaluated their in vivo functions.

Customized Strategies for High-Yield Purification of Retinal Pigment Epithelial Cells Differentiated from Different Stem Cell Sources

[Scientific Reports] Researchers systematically compared five different retinal pigment epithelial purification methods, including manual, enzymatic, flow cytometry-based sorting or combinations thereof for parameters including cell throughput, yield, purity and functionality.

Dominant Role of DNA Methylation over H3K9me3 for IAP Silencing in Endoderm

[Nature Communications] Scientists showed that conditional knock-out of Setdb1 in mouse embryonic endoderm resulted in endogenous retroviruse de-repression in visceral endoderm descendants and did not occur in definitive endoderm.

CTCF Acetylation at Lysine 20 Is Required for the Early Cardiac Mesoderm Differentiation of Embryonic Stem Cells

[Cell Regeneration] Investigators showed that CCCTC-binding factor (CTCF) could be acetylated at lysine 20 (CTCF-K20) by CREB-binding protein (CBP) and deacetylated by histone deacetylase 6 (HDAC6). CTCF-K20 was required for the CTCF interaction with CBP.

Evolutionary Origin of Vertebrate OCT4/POU5 Functions in Supporting Pluripotency

[Nature Communications] By coupling evolutionary sequence analysis with functional studies in mESCs, the investigators found that the ability of POU5 proteins to support pluripotency originated in the gnathostome lineage, prior to the generation of two paralogues, Pou5f1 and Pou5f3 via gene duplication.

PIM3-AMPK-HDAC4/5 Axis Restricts MuERVL-Marked 2-Cell-Like State in Embryonic Stem Cells

[Stem Cell Reports] Downregulation, deletion, or inhibition of PIM3 activated MuERVL, 2-cell genes, and trophectodermal genes in ESCs. By screening PIM3-regulated pathways, scientists discovered AMPK as its key target.

STRAIGHT-IN Enables High-Throughput Targeting of Large DNA Payloads in Human Pluripotent Stem Cells

[Cell Reports Methods] The authors merged the strengths of different classes of site-specific recombinases and combined these with CRISPR-Cas9-mediated homologous recombination to develop a strategy for stringent site-specific replacement of genomic fragments at least 50 kb in size in hiPSCs.

LncRNA-Smad7 Mediates Cross-Talk between Nodal/TGF-β and BMP Signaling to Regulate Cell Fate Determination of Pluripotent and Multipotent Cells

[Nucleic Acids Research] Scientists showed that the Nodal induced lncRNA-Smad7 regulated cell fate determination via repression of BMP signaling in mESCs.

Passage Number Affects Differentiation of Sensory Neurons from Human Induced Pluripotent Stem Cells

[Scientific Reports] Scientists investigated the effect of passage number on iPSC differentiation to optimize the generation of sensory neurons (iPSC-dSNs). Three iPSC lines reprogrammed from the peripheral blood of three donors were differentiated into iPSC-dSNs at passage numbers within each of the following ranges: 5–10, 20–26, and 30–38.

Modeling Urea Cycle Disorders Using iPSCs

[NPJ Regenerative Medicine] The authors summarized the progress made thus far in generating 2D and 3D iPSCs models for urea cycle disorders (UCDs), the challenges encountered, and how iPSCs offer future avenues for innovation in developing the next-generation of therapies for UCDs.

University of Manchester To Collaborate with IIT Kharagpur and Tata Research Centre To Contribute to Cancer Research

[University of Manchester (Education Times)] The University of Manchester will collaborate with several Indian institutes, medical research centres and corporates to boost initiatives to find solutions to global problems. In Bengaluru, the delegation will visit the National Centre for Biological Sciences to build on links in areas such as stem cell research.

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