lbeveridge

[Neuro-Oncology] Hybridoma cells were generated by immunizing BALB/c mice with EVA1-Fc fusion protein. The reactivity of the supernatant from these hybridoma cells was examined using EVA1-overexpressing cells and glioblastoma-initiating cells. Candidate antibodies were further selected using Biacore surface plasmon resonance analysis and two cytotoxicity assays, antibody-dependent cell cytotoxicity and complement-dependent cytotoxicity

[Nature] Investigators provide the mechanistic explanation for this in a requirement for mutant RAS to specifically transform committed progenitors of the myelomonocytic lineage harboring previously acquired driver mutations, showing that advanced leukemic clones can originate from a different cell type in the hematopoietic hierarchy than ancestral clones.

[FierceBiotech] Using high-parametric tissue imaging with a 30-plex antibody panel, investigators analyzed human lung samples from 6 to 13 post-conception weeks, generating data from over 2 million cells across five developmental timepoints.

[PLOS Biology] Scientists describe the role of MICU2 in cell proliferation and invasion using in vitro and in vivo models of human CRC. Transcriptomic analysis demonstrated an increase in MICU2 expression and the MICU2/MICU1 ratio in advanced CRC and CRC-derived metastases.

[Scientific Reports] Rectal organoid and mucosal cells from endoscopic biopsies of each patient having perianal fistulizing CD or no disease controls were prepared for and sequenced at the single cell level.

[Cell Reports Medicine] Investigators integrated clonal kinetics with transcriptomic heterogeneity resolved by single-cell omics to interrogate cellular dynamics of non-transduced and transduced T cells, in the infusion product and at the CAR-T cell expansion peak in five B cell acute lymphoblastic leukemia patients treated with CD19 CAR-T cells