Noshin Noorjahan

[Stem Cell Reports] RNA sequencing and lineage fate analysis revealed that budesonide, a glucocorticoid drug widely used to treat asthma, prevented ESC aggregates to break symmetry by counteracting exit from pluripotency and modifying the expression of a large set of genes associated with cell migration, A-P axis formation, and WNT signaling.

[Journal of Cellular Physiology] Researchers established a fast and efficient induction system of porcine iPSCs from porcine Sertoli cells via forced expression of pig Yamanaka factors.

[Cytotherapy] The authors focused on identifying markers for iPSC impurities in iPSC-derived cardiomyocytes and validated the selected genes by reverse transcription quantitative polymerase chain reaction.

[Biophysical Journal] Using a novel coarse-grained model and molecular dynamics simulations, scientists probed the association of Sox2 and Oct4 proteins that showed clustered binding at the entry-exit region of a nucleosome.

[International Journal of Molecular Medicine] Green fluorescent protein+/iPS‑like colony generation was facilitated by the small molecules CHIR99021 and RepSox during lentivirus‑mediated reprogramming of six transcription factors in rat embryonic fibroblasts. The generation of iPSCs was detected by reverse transcription‑PCR, immunofluorescence, and western blot analysis.

[International Journal of Stem Cells] Investigators used three types of DNA methyltransferase (Dnmt) knockout (KO) ESCs, including Dnmt1 KO, Dnmt3a/3b double KO, and Dnmt1/3a/3b triple KO, to investigate the role of Dnmts in neural differentiation of ESCs.