Investigators review the research progress made in the isolation and characterization of liver cancer stem cells by considering a wide range of cell surface markers and sorting methods, as applied to side populations, microsphere cultures and the gradient centrifugation method.
[Stem Cell Reviews and Reports]
To determine how CD117 expression and activation by its ligand stem cell factor alters prostate cancer aggressiveness, researchers used C4-2 and PC3-mm human prostate cancer cells, which contained a CD117+ subpopulation.
6445212 5FRKQI8W items 1 apa default asc 1
Harris, K. S., Shi, L., Foster, B. M., Mobley, M. E., Elliott, P. L., Song, C. J., Watabe, K., Langefeld, C. D., & Kerr, B. A. (2021). CD117/c-kit defines a prostate CSC-like subpopulation driving progression and TKI resistance. Scientific Reports, 11(1), 1465. https://doi.org/10.1038/s41598-021-81126-6 Cite
The authors found that interfering with expression of the receptor for the lactogenic hormone prolactin in breast cancer cells representative of the luminal and epithelial breast cancer subtypes resulted in loss of their differentiation state, enriched for stem-like cell subpopulations, and increased their tumorigenic capacity in a subtype-specific manner.
6807162 U934RQ67 items 1 apa default asc 1
Shams, A., Binothman, N., Boudreault, J., Wang, N., Shams, F., Hamam, D., Tian, J., Moamer, A., Dai, M., Lebrun, J.-J., & Ali, S. (2021). Prolactin receptor-driven combined luminal and epithelial differentiation in breast cancer restricts plasticity, stemness, tumorigenesis and metastasis. Oncogenesis, 10(1), 1–16. https://doi.org/10.1038/s41389-020-00297-5 Cite
Investigators found that high expression of desmoglein2, a component of desmosome-mediated intercellular adhesion complexes, promoted tumor growth, increased the prevalence of circulating tumor cell clusters, and facilitated distant organ colonization.
[Proceedings of the National Academy of Sciences of the United States of America]
6807162 R36HPIH8 items 1 apa default asc 1
Chang, P.-H., Chen, M.-C., Tsai, Y.-P., Tan, G. Y. T., Hsu, P.-H., Jeng, Y.-M., Tsai, Y.-F., Yang, M.-H., & Hwang-Verslues, W. W. (2021). Interplay between desmoglein2 and hypoxia controls metastasis in breast cancer. Proceedings of the National Academy of Sciences, 118(3). https://doi.org/10.1073/pnas.2014408118 Cite
The authors performed an in silico study of ADAMTS1 and endothelial markers in human gliomas, providing the basis to further assess these molecules in several primary glioblastoma-initiating cells and established glioblastoma cells with the ability to acquire an endothelial-like phenotype.
6445212 ADAMTS1SupportsEndothelialPlasticityofGliobla items 1 apa default asc 1
Investigators summarize the impact of hypoxia and acidic stress on glioma stem cell signaling and biologic phenotypes, and potential methods to inhibit these pathways.
6445212 FFSYCM59 items 1 apa default asc 1
The authors established a CAM-LDA assay able to rapidly reproduce tumor specificities- in particular, the ability of the small population of cancer stem cells to form tumors.
[International Journal of Molecular Sciences]
6445212 H62MV2QF items 1 apa default asc 1
The effects of ACT001 on cell survival of normal human astrocytes and patient-derived glioma stem-like cells were evaluated. RNA-Seq were performed to detect differentially expressed genes.
6445212 C9TRPK5G items 1 apa default asc 1
Researchers used droplet digital PCR or PCR for antigen receptor rearrangement, to explore tumor-specific point mutations, copy number alterations, and chromosomal rearrangements in the plasma of cancer-affected dogs. They detected circulating tumor DNA in 21/23 of histiocytic sarcoma, 2/8of oral melanoma, and 12/13 of lymphoma cases.
6445212 IT8SCWWZ items 1 apa default asc 1
Prouteau, A., Denis, J. A., De Fornel, P., Cadieu, E., Derrien, T., Kergal, C., Botherel, N., Ulvé, R., Rault, M., Bouzidi, A., François, R., Dorso, L., Lespagnol, A., Devauchelle, P., Abadie, J., André, C., & Hédan, B. (2021). Circulating tumor DNA is detectable in canine histiocytic sarcoma, oral malignant melanoma, and multicentric lymphoma. Scientific Reports, 11(1), 877. https://doi.org/10.1038/s41598-020-80332-y Cite
Scientists found that the CCAAT/Enhancer-binding protein delta (CEBPD) protein levels in glioblastoma patients were significantly increased and further contributed to temozolamide resistance by promoting glioma-like stem cell formation.
[Cell Death Discovery]
6445212 34GWVJAT items 1 apa default asc 1
Wang, S.-M., Lin, W.-C., Lin, H.-Y., Chen, Y.-L., Ko, C.-Y., & Wang, J.-M. (2021). CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 activation for temozolomide resistance in glioblastoma. Cell Death Discovery, 7(1), 1–11. https://doi.org/10.1038/s41420-020-00399-4 Cite
Investigators report a nanotherapeutic strategy to kill cancer stem cells in tumors using nanoparticles that were co-loaded with the differentiation-inducing agent, all-trans retinoic acid, and the chemotherapeutic drug, camptothecin.