Isolation and Enrichment of Melanocytes from Human Corneal Limbus Using CD117 (C-Kit) as Selection Marker

Investigators report on a facile protocol for the efficient isolation and enrichment of pure populations of human limbal melanocytes by fluorescence-activated cell sorting using antibodies raised against the cell surface marker CD117.
[Scientific Reports]
Polisetti, N., Schlötzer-Schrehardt, U., Reinhard, T., & Schlunck, G. (2020). Isolation and enrichment of melanocytes from human corneal limbus using CD117 (c-Kit) as selection marker. Scientific Reports, 10(1), 17588. https://doi.org/10.1038/s41598-020-74869-1 Cite
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Dermal Fibroblasts Have Different Extracellular Matrix Profiles Induced by TGF-β, PDGF and IL-6 in a Model for Skin Fibrosis

Researchers investigated the expression of extracellular matrix by dermal fibroblast mimicking fibrotic skin diseases as systemic sclerosis using clinically validated biomarkers.
[Scientific Reports]
Juhl, P., Bondesen, S., Hawkins, C. L., Karsdal, M. A., Bay-Jensen, A.-C., Davies, M. J., & Siebuhr, A. S. (2020). Dermal fibroblasts have different extracellular matrix profiles induced by TGF-β, PDGF and IL-6 in a model for skin fibrosis. Scientific Reports, 10(1), 17300. https://doi.org/10.1038/s41598-020-74179-6 Cite
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Integrative Transcriptomic Analysis for Linking Acute Stress Responses to Squamous Cell Carcinoma Development

In vitro, Oncostatin M promoted invasiveness of keratinocytes and cutaneous squamous cell carcinoma cells and suppressed apoptosis of irradiated keratinocytes.
[Scientific Reports]
Nguyen, T. N., Rajapakshe, K., Nicholas, C., Tordesillas, L., Ehli, E. A., Davis, C. M., Coarfa, C., Flores, E. R., Dickinson, S. E., Curiel-Lewandrowski, C., & Tsai, K. Y. (2020). Integrative transcriptomic analysis for linking acute stress responses to squamous cell carcinoma development. Scientific Reports, 10(1), 17209. https://doi.org/10.1038/s41598-020-74051-7 Cite
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Evelo Biosciences Completes Enrollment in Phase Ib Clinical Trial Cohort Evaluating EDP1815 in Atopic Dermatitis

Evelo Biosciences, Inc. completed enrollment of 24 patients in the Phase Ib clinical trial cohort evaluating EDP1815 in mild to moderate atopic dermatitis. EDP1815 is an investigational oral biologic in development for the treatment of inflammatory diseases
[Evelo Biosciences, Inc.]
Press Release
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NOTCH1 Gene Amplification Promotes Expansion of Cancer Associated Fibroblast Populations in Human Skin

Researchers report that cancer associated fibroblasts from skin squamous cell carcinomas displayed chromosomal alterations, with heterogeneous NOTCH1 gene amplification and overexpression that also occurred, to a lesser extent, in dermal fibroblasts of apparently unaffected skin.
[Nature Communications]
Katarkar, A., Bottoni, G., Clocchiatti, A., Goruppi, S., Bordignon, P., Lazzaroni, F., Gregnanin, I., Ostano, P., Neel, V., & Dotto, G. P. (2020). NOTCH1 gene amplification promotes expansion of Cancer Associated Fibroblast populations in human skin. Nature Communications, 11(1), 5126. https://doi.org/10.1038/s41467-020-18919-2 Cite
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Second-Strand Synthesis-Based Massively Parallel scRNA-Seq Reveals Cellular States and Molecular Features of Human Inflammatory Skin Pathologies

Investigators used Seq-Well S3 (“Second-Strand Synthesis”) to chart the transcriptional landscape of five human inflammatory skin diseases, thus providing a resource for the further study of human skin inflammation.
[Immunity]
Hughes, T. K., Wadsworth, M. H., Gierahn, T. M., Do, T., Weiss, D., Andrade, P. R., Ma, F., de Andrade Silva, B. J., Shao, S., Tsoi, L. C., Ordovas-Montanes, J., Gudjonsson, J. E., Modlin, R. L., Love, J. C., & Shalek, A. K. (2020). Second-Strand Synthesis-Based Massively Parallel scRNA-Seq Reveals Cellular States and Molecular Features of Human Inflammatory Skin Pathologies. Immunity, 53(4), 878-894.e7. https://doi.org/10.1016/j.immuni.2020.09.015 Cite
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Genome-Wide RNAi Screen for Genes Regulating Glycolytic Response to Vemurafenib in BRAFV600

Scientists presented a multiparameter genome-wide siRNA screening dataset of genes that when depleted, improved the viability and glycolytic response to vemurafenib in BRAFV600 mutated melanoma cells.
[Scientific Data]
Smith, L. K., Parmenter, T., Gould, C. M., Madhamshettiwar, P. B., Sheppard, K. E., Simpson, K. J., & McArthur, G. A. (2020). Genome-wide RNAi screen for genes regulating glycolytic response to vemurafenib in BRAF V600 melanoma cells. Scientific Data, 7(1), 339. https://doi.org/10.1038/s41597-020-00683-z Cite
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The Arp2/3 Complex Is Critical for Colonisation of the Mouse Skin by Melanoblasts

Arp3 depletion in the melanocyte lineage resulted in severe pigmentation defects in dorsal and ventral regions of the mouse skin.
[Development]
Papalazarou, V., Swaminathan, K., Jaber-Hijazi, F., Spence, H., Lahmann, I., Nixon, C., Salmeron-Sanchez, M., Arnold, H.-H., Rottner, K., & Machesky, L. M. (2020). The Arp2/3 complex is critical for colonisation of the mouse skin by melanoblasts. Development. https://doi.org/10.1242/dev.194555 Cite
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Nicotinamide Inhibits Melanoma In Vitro and In Vivo

The anti-melanoma activity of Nicotinamide, the amide form of Niacin, was assessed in vitro and in vivo.
[Journal of Experimental & Clinical Cancer Research]
Scatozza, F., Moschella, F., D’Arcangelo, D., Rossi, S., Tabolacci, C., Giampietri, C., Proietti, E., Facchiano, F., & Facchiano, A. (2020). Nicotinamide inhibits melanoma in vitro and in vivo. Journal of Experimental & Clinical Cancer Research, 39(1), 211. https://doi.org/10.1186/s13046-020-01719-3 Cite
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Establishment and Validation of an In Vitro Co-Culture Model for Oral Cell Lines Using Human PBMC-Derived Osteoclasts, Osteoblasts, Fibroblasts and Keratinocytes

Researchers investigated the viability and proliferation of osteoblasts, fibroblasts and oral keratinocytes under stratified medium modification and assessed the differentiation of osteoclasts in each co-culture.
[Scientific Reports]
Establishment and validation of an in vitro co-culture model for oral cell lines using human PBMC-derived osteoclasts, osteoblasts, fibroblasts and keratinocytes | Scientific Reports. (n.d.). Retrieved October 8, 2020, from https://www.nature.com/articles/s41598-020-73941-0 Cite
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AP-1 and TGFß Cooperativity Drives Non-Canonical Hedgehog Signaling in Resistant Basal Cell Carcinoma

The authors used single cell RNA-sequencing of patient tumors to identify three prognostic surface markers (LYPD3, TACSTD2, and LY6D) which correlated with myocardin-related transcription factor (nMRTF) and resistance to Smoothened inhibitors. The nMRTF cell state resembled transit-amplifying cells of the hair follicle matrix, with AP-1 and TGFß cooperativity driving nMRTF activation.
[Nature Communications]
Yao, C. D., Haensel, D., Gaddam, S., Patel, T., Atwood, S. X., Sarin, K. Y., Whitson, R. J., McKellar, S., Shankar, G., Aasi, S., Rieger, K., & Oro, A. E. (2020). AP-1 and TGFß cooperativity drives non-canonical Hedgehog signaling in resistant basal cell carcinoma. Nature Communications, 11(1), 5079. https://doi.org/10.1038/s41467-020-18762-5 Cite
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