Brahma Safeguards Canalization of Cardiac Mesoderm Differentiation

Scientists showed that the BRG1/BRM-associated factor chromatin-remodeling complex ATPase gene Brm safeguarded cell identity during directed cardiogenesis of mouse ESCs.
[Nature]
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Improved gRNA Secondary Structures Allow Editing of Target Sites Resistant to CRISPR-Cas9 Cleavage

Researchers engineered gRNAs with highly stable hairpins in their constant parts and further enhanced their stability by chemical modifications.
[Nature Communications]
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SETDB1/NSD-Dependent H3K9me3/H3K36me3 Dual Heterochromatin Maintains Gene Expression Profiles by Bookmarking Poised Enhancers

The authors identified thousands of dual regions in mouse ESCs that relied on the histone methyltransferases SET domain bifurcated 1 (SETDB1) and nuclear set domain (NSD)-containing proteins to generate H3K9me3 and H3K36me3, respectively.
[Molecular Cell]
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Inducing Somatic Cells into Pluripotent Stem Cells Is an Important Platform to Study the Mechanism of Early Embryonic Development

Scientists introduce the research advance of both somatic cell reprogramming and early embryonic development, indicating that the mechanisms of iPSCs also shares common features with that of early embryonic development in several aspects.
[Molecular Reproduction and Development]
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iMyoblasts for Ex Vivo and In Vivo Investigations of Human Myogenesis and Disease Modeling

Skeletal muscle myoblasts (iMyoblasts) were generated from human iiPSCs using an efficient and reliable transgene-free induction and stem cell selection protocol.
[eLife]
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Loss of plac8 Expression Rapidly Leads Pluripotent Stem Cells to Enter Active State during Planarian Regeneration

The authors suggested that Djplac8-A acted as a molecular switch of piwi+ stem cells for entry into the regenerative state after amputation.
[Development]
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CRISPR Activation Enables High-Fidelity Reprogramming into Human Pluripotent Stem Cells

Researchers described a high-efficiency reprogramming of human somatic cells into iPSCs using optimized CRISPR activation.
[Stem Cell Reports]
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Haploidy in Somatic Cells Is Induced by Mature Oocytes in Mice

Investigators demonstrated that the replacement of meiotic spindles in mature metaphases II arrested oocytes with nuclei of somatic cells in the G0/G1 stage of cell cycle resulted in the formation of de novo spindles consisting of somatic homologous chromosomes comprising of single chromatids.
[Communications Biology]
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A Large Scale Mass Spectrometry-Based Histone Screening for Assessing Epigenetic Developmental Toxicity

Scientists developed a mass spectrometry-based proof-of-concept to assess a histone code screening assay capable of simultaneously detecting multiple hPTM-changes in human ESCs.
[Scientific Reports]
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Parallel Functional Assessment of m6A Sites in Human Endodermal Differentiation with Base Editor Screens

The authors developed an adenine base editor-based strategy to systematically identify functional m6A sites that controlled lineage decisions of human #ESCs.
[Nature Communications]
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Matrix Enrichment by Black Phosphorus Improves Ionization and Reproducibility of Mass Spectrometry of Intact Cells, Peptides, and Amino Acids

Scientists found that enrichment of common organic matrices by black phosphorus could improve discrimination data analysis by enhancing peak intensity and reproducibility of mass spectra acquired from intact cells.
[Scientific Reports]
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