iPSC-Derived Progenitor Stromal Cells Provide New Insights into Aberrant Musculoskeletal Development and Resistance to Cancer in Down Syndrome

To gain new insights into aberrant Down Syndrome (DS) development during early stages of mesoderm formation and its possible connection to lower solid tumor prevalence, the authors developed the first model of two types of DS iPSC-derived stromal cells.
[Scientific Reports]
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Active Turnover of Genomic Methylcytosine in Pluripotent Cells

Scientists used metabolic labeling with stable isotopes and mass spectrometry to quantitatively address the global turnover of genomic 5-methyl-2′-deoxycytidine, 5-hydroxymethyl-2′-deoxycytidine and 5-formyl-2′-deoxycytidine across mouse pluripotent cell states.
[Nature Chemical Biology]
Spada, F., Schiffers, S., Kirchner, A., Zhang, Y., Arista, G., Kosmatchev, O., Korytiakova, E., Rahimoff, R., Ebert, C., & Carell, T. (2020). Active turnover of genomic methylcytosine in pluripotent cells. Nature Chemical Biology, 1–9. https://doi.org/10.1038/s41589-020-0621-y Cite
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High-Throughput 3D Screening for Differentiation of hPSC-Derived Cell Therapy Candidates

Researchers demonstrated a high-throughput 3D culture platform used to systematically screen 1200 culture conditions with varying doses, durations, dynamics, and combinations of signaling cues to derive oligodendrocyte progenitor cells and midbrain dopaminergic neurons from human PSCs.
[Science Advances]
Muckom, R., Bao, X., Tran, E., Chen, E., Murugappan, A., Dordick, J. S., Clark, D. S., & Schaffer, D. V. (2020). High-throughput 3D screening for differentiation of hPSC-derived cell therapy candidates. Science Advances, 6(32), eaaz1457. https://doi.org/10.1126/sciadv.aaz1457 Cite
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Three-Dimensional Otic Neuronal Progenitor Spheroids Derived from Human Embryonic Stem Cells

Investigators generated human ESC-derived otic neuronal progenitor spheroids—an aggregate form conducive to differentiation, transplantation, and prolonged cell survival—to optimize conditions for their transplantation.
[tissue engineering]
Heuer, R. A., Nella, K. T., Chang, H.-T., Coots, K. S., Oleksijew, A. M., Roque, C. B., Silva, L. H. A., McGuire, T. L., Homma, K., & Matsuoka, A. J. (2020). Three-Dimensional Otic Neuronal Progenitor Spheroids Derived from Human Embryonic Stem Cells. Tissue Engineering Part A. https://doi.org/10.1089/ten.tea.2020.0078 Cite
Abstract

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Aligned Nanofiber Scaffolds Improve Functionality of Cardiomyocytes Differentiated from Human Induced Pluripotent Stem Cell-Derived Cardiac Progenitor Cells

Scientists showed that the 3D culture with aligned nanofiber scaffolds, mimicing the architecture of the extracellular matrix of the heart, improved the differentiation of iPSC-cardiac progenitor cells to functional cardiomyocytes induced by Wnt inhibition, as shown with increased number of cardiac Troponin T-positive cells and synchronized intracellular Ca2+ oscillation.
[Scientific Reports]
Ding, M., Andersson, H., Martinsson, S., Sabirsh, A., Jonebring, A., Wang, Q.-D., Plowright, A. T., & Drowley, L. (2020). Aligned nanofiber scaffolds improve functionality of cardiomyocytes differentiated from human induced pluripotent stem cell-derived cardiac progenitor cells. Scientific Reports, 10(1), 13575. https://doi.org/10.1038/s41598-020-70547-4 Cite
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MicroRNA-Dependent Inhibition of PFN2 Orchestrates ERK Activation and Pluripotent State Transitions by Regulating Endocytosis

The authors showed that the miR-290-PFN2 pathway regulates many aspects of ESC biology. In the absence of miRNAs, PFN2 was up-regulated in ESCs, with a resulting decrease in endocytosis.
[Proceedings of the National Academy of Sciences of the United States of America]
Sangokoya, C., & Blelloch, R. (2020). MicroRNA-dependent inhibition of PFN2 orchestrates ERK activation and pluripotent state transitions by regulating endocytosis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.2002750117 Cite
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Exosomes — Beyond Stem Cells for Cardiac Protection and Repair

Scientists discuss the therapeutic benefit of exosomes secreted by stem and progenitor cells in preclinical models of ischemic heart disease.
[Stem Cells]
Balbi, C., & Vassalli, G. (n.d.). Exosomes — Beyond Stem Cells for Cardiac Protection and Repair. STEM CELLS, n/a(n/a). https://doi.org/10.1002/stem.3261 Cite
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HGF and IL-10 Expressing ALB::GFP Reporter Cells Generated From iPSCs Show Robust Anti-Fibrotic Property in Acute Fibrotic Liver Model

Induced hepatocyte-like (iHep) cells were generated from induced pluripotent stem cells integrated with the albumin reporter gene. The therapeutic properties of these iHep cells were investigated after transplantation in fibrotic liver tissues of a mouse model.
[Stem Cell Research & Therapy]
Full Article

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Modeling SHH-Driven Medulloblastoma with Patient iPS Cell-Derived Neural Stem Cells

Investigators describe a medulloblastoma model using induced pluripotent stem cell-derived human neuroepithelial stem cells generated from a Gorlin syndrome patient carrying a germline mutation in the sonic hedgehog receptor PTCH1.
[Proceedings of the National Academy of Sciences of the United States of America]
Susanto, E., Navarro, A. M., Zhou, L., Sundström, A., Bree, N. van, Stantic, M., Moslem, M., Tailor, J., Rietdijk, J., Zubillaga, V., Hübner, J.-M., Weishaupt, H., Wolfsberger, J., Alafuzoff, I., Nordgren, A., Magnaldo, T., Siesjö, P., Johnsen, J. I., Kool, M., … Wilhelm, M. (2020). Modeling SHH-driven medulloblastoma with patient iPS cell-derived neural stem cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1920521117 Cite
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iPSC-Derived Progenitor Stromal Cells Provide New Insights into Aberrant Musculoskeletal Development and Resistance to Cancer in Down Syndrome

To gain new insights into aberrant Down Syndrome (DS) development during early stages of mesoderm formation and its possible connection to lower solid tumor prevalence, the authors developed the first model of two types of DS iPSC-derived stromal cells.
[Scientific Reports]
Galat, Y., Perepitchka, M., Elcheva, I., Iannaccone, S., Iannaccone, P. M., & Galat, V. (2020). iPSC-derived progenitor stromal cells provide new insights into aberrant musculoskeletal development and resistance to cancer in down syndrome. Scientific Reports, 10(1), 13252. https://doi.org/10.1038/s41598-020-69418-9 Cite
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Inhibition of Protein Kinase D by CID755673 Promotes Maintenance of the Pluripotency of Embryonic Stem Cells

Investigators showed that the protein kinase D inhibitor CID755673 was able to maintain the undifferentiated state of mouse ESCs in combination with the mitogen-activated protein kinase kinase inhibitor.
[Development]
Zhu, Z., Zhang, Y., Wang, X., Wang, X., & Ye, S.-D. (2020). Inhibition of protein kinase D by CID755673 promotes maintenance of the pluripotency of embryonic stem cells. Development. https://doi.org/10.1242/dev.185264 Cite
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PR-DUB Maintains Expression of Critical Genes through FOXK1/2 and ASXL1/2/3-Dependent Recruitment to Chromatin and H2AK119ub1 Deubiquitination

Scientists showed that BAP1 was dependent on the ASXL proteins and FOXK1/2 in facilitating gene activation across the genome. Although PR-DUB previously was shown to cooperate with PRC2, they observed minimal overlap and functional interaction between BAP1 and PRC2 in embryonic stem cells.
[Genome Research]
Kolovos, P., Nishimura, K., Sankar, A., Sidoli, S., Cloos, P. A., Helin, K., & Christensen, J. (2020). PR-DUB maintains expression of critical genes through FOXK1/2 and ASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1 deubiquitination. Genome Research, gr.261016.120. https://doi.org/10.1101/gr.261016.120 Cite
Abstract

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