Activated Microglia Cause Metabolic Disruptions in Developmental Cortical Interneurons That Persist in Interneurons From Individuals With Schizophrenia

Researchers generated developmental cortical interneurons — which are known to be affected in schizophrenia when matured — from induced pluripotent stem cells derived from healthy controls and individuals with schizophrenia and co-cultured them with or without activated microglia.
[Nature Neuroscience]
Activated microglia cause metabolic disruptions in developmental cortical interneurons that persist in interneurons from individuals with schizophrenia | Nature Neuroscience. (n.d.). Retrieved October 23, 2020, from https://www.nature.com/articles/s41593-020-00724-1 Cite
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Herpes Simplex Virus Type 1 Infection Leads to Neurodevelopmental Disorder-Associated Neuropathological Changes

Researchers generated in vitro neurodevelopmental disorder models including human induced pluripotent stem cell based monolayer neuronal differentiation, 3D neuroepithelial bud, and 3D cerebral organoid to study fetal brain development and the potential neuropathological effects induced by the HSV-1 infections.
[Plos Pathogens]
Herpes simplex virus type 1 infection leads to neurodevelopmental disorder-associated neuropathological changes. (n.d.). Retrieved October 23, 2020, from https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1008899 Cite
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Single-Cell RNA-Seq Reveals that CD9 Is a Negative Marker of Glucose-Responsive Pancreatic β-like Cells Derived from Human Pluripotent Stem Cells

Scientists observed more CD9+C-PEPTIDE+ β cells in the fetal than in the adult cadaveric islets and more Ki67+ proliferating cells among CD9+ fetal β cells. Their experiments showed CD9 as a cell-surface marker for negative enrichment of glucose-responsive β-like cells differentiated from human PSCs.
[Stem Cell Reports]
Li, X., Yang, K. Y., Chan, V. W., Leung, K. T., Zhang, X.-B., Wong, A. S., Chong, C. C. N., Wang, C. C., Ku, M., & Lui, K. O. (2020). Single-Cell RNA-Seq Reveals that CD9 Is a Negative Marker of Glucose-Responsive Pancreatic β-like Cells Derived from Human Pluripotent Stem Cells. Stem Cell Reports, 0(0). https://doi.org/10.1016/j.stemcr.2020.09.009 Cite
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Elimination of Mutant mtDNA by an Optimized mpTALEN Restores Differentiation Capacities of Heteroplasmic MELAS-iPSCs

Researchers refined a previously generated G13513A mtDNA-targeted platinum transcription activator-like effector nuclease (G13513A-mpTALEN) to more efficiently manipulate mtDNA heteroplasmy in mitochondrial encephalopathy, lactic acidosis, and stroke-like episodes (MELAS)-iPSCs.
[Molecular Therapy-Methods & Clinical Development]
Yahata, N., Boda, H., & Hata, R. (2020). Elimination of mutant mtDNA by an optimized mpTALEN restores differentiation capacities of heteroplasmic MELAS-iPSCs. Molecular Therapy - Methods & Clinical Development, 0(0). https://doi.org/10.1016/j.omtm.2020.10.017 Cite
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Live Visualization of ERK Activity in the Mouse Blastocyst Reveals Lineage-Specific Signaling Dynamics

To address whether differential signaling is associated with particular cell fates and states, researchers generated a targeted mouse line expressing an ERK-kinase translocation reporter that enabled live quantification of ERK activity at single-cell resolution.
[Developmental Cell]
Simon, C. S., Rahman, S., Raina, D., Schröter, C., & Hadjantonakis, A.-K. (2020). Live Visualization of ERK Activity in the Mouse Blastocyst Reveals Lineage-Specific Signaling Dynamics. Developmental Cell, 0(0). https://doi.org/10.1016/j.devcel.2020.09.030 Cite
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Cell-Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo

Endogenous tagging of Nanog in ESCs revealed that ERK inhibition promoted enhanced stabilization of NANOG protein after mitosis. Scientists showed that cell cycle, signaling, and differentiation are coordinated during preimplantation development.
[Developmental Cell]
Pokrass, M. J., Ryan, K. A., Xin, T., Pielstick, B., Timp, W., Greco, V., & Regot, S. (2020). Cell-Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo. Developmental Cell, 0(0). https://doi.org/10.1016/j.devcel.2020.09.013 Cite
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Comparison of 10 Control hPSC Lines for Drug Screening in an Engineered Heart Tissue Format

Scientists investigated ten different control hiPSC-cardiomyocyte (CM) lines and analyzed function and suitability for drug screening. Five commercial and five academic hPSC-CM lines were casted in engineered heart tissue format.
[Stem Cell Reports]
Mannhardt, I., Saleem, U., Mosqueira, D., Loos, M. F., Ulmer, B. M., Lemoine, M. D., Larsson, C., Améen, C., Korte, T. de, Vlaming, M. L. H., Harris, K., Clements, P., Denning, C., Hansen, A., & Eschenhagen, T. (2020). Comparison of 10 Control hPSC Lines for Drug Screening in an Engineered Heart Tissue Format. Stem Cell Reports, 15(4), 983–998. https://doi.org/10.1016/j.stemcr.2020.09.002 Cite
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Development and Application of High-Throughput Single Cell Lipid Profiling: A Study of SNCA-A53T Human Dopamine Neurons

The authors identified and isolated human iPSC-dopamine neurons with a TH-RFP reporter. Using liquid extraction surface analysis (LESA) coupled with high-resolution mass spectrometry they developed a high-throughput method for untargeted single cell lipid profiling.
[iScience]
Snowden, S. G., Fernandes, H. J. R., Kent, J., Foskolou, S., Tate, P., Field, S. F., Metzakopian, E., & Koulman, A. (2020). Development and application of high-throughput single cell lipid profiling: a study of SNCA-A53T human dopamine neurons. IScience, 0(0). https://doi.org/10.1016/j.isci.2020.101703 Cite
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Human Stem Cell-Derived Oligodendrocytes: From Humanized Animal Models to Cell Therapy in Myelin Diseases

The authors discuss the anatomical and functional benefit of grafted iPSC-progenitors over their brain counterparts, their use in disease modeling and the missing gaps that still prevent to study their biology in the most integrated way, and to translate iPSC-stem cell based therapy to the clinic.
[Seminars in Cell & Developmental Biology]
Mozafari, S., & Baron-Van Evercooren, A. (2020). Human stem cell-derived oligodendrocytes: From humanized animal models to cell therapy in myelin diseases. Seminars in Cell & Developmental Biology. https://doi.org/10.1016/j.semcdb.2020.09.011 Cite
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Lineage Cell Therapeutics and Cancer Research UK Announce Encouraging Preliminary Phase I Study Results with VAC2 for the Treatment of Non-Small Cell Lung Cancer

Lineage Cell Therapeutics, Inc. and Cancer Research UK have announced encouraging preliminary results from an ongoing Phase I clinical study of VAC2 in non-small cell lung cancer (NSCLC). Produced from a pluripotent cell technology using a directed differentiation method, VAC2 has demonstrated remarkably potent induction of immune responses in all patients dosed to date.
[nan]
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When Glia Meet Induced Pluripotent Stem Cells (iPSCs)

Investigators summarize the basic characteristics of glial cells, with the focus on their classical functions, heterogeneity, and uniqueness in human species. They further review the findings from recent studies that use iPSC-derived glial cells for central nervous system disease modeling.
[Molecular and Cellular Neuroscience]
Li, L., & Shi, Y. (2020). When Glia Meet Induced Pluripotent Stem Cells (iPSCs). Molecular and Cellular Neuroscience, 103565. https://doi.org/10.1016/j.mcn.2020.103565 Cite
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