Signaling Inhibitors Accelerate the Conversion of Mouse iPS Cells into Cancer Stem Cells in the Tumor Microenvironment

Inhibition of Erk1/2, tyrosine kinase, and/or GSK-3β was implied to be involved in the enhancement of the PI3K-AKT signaling pathway in the undifferentiated cells, resulting in the sustained stemness, and subsequent conversion of mouse iPSCs into cancer stem cells in the tumor microenvironment.
[Scientific Reports]
Du, J., Xu, Y., Sasada, S., Oo, A. K. K., Hassan, G., Mahmud, H., Khayrani, A. C., Alam, M. J., Kumon, K., Uesaki, R., Afify, S. M., Mansour, H. M., Nair, N., Zahra, M. H., Seno, A., Okada, N., Chen, L., Yan, T., & Seno, M. (2020). Signaling Inhibitors Accelerate the Conversion of mouse iPS Cells into Cancer Stem Cells in the Tumor Microenvironment. Scientific Reports, 10(1), 9955. https://doi.org/10.1038/s41598-020-66471-2 Cite
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Derivation of Oocyte-Like Cells from Putative Embryonic Stem Cells and Parthenogenetically Activated into Blastocysts in Goat

Scientists demonstrated that goat putative ESCs were successfully in vitro differentiated into primordial germ cells and oocyte-like cells using bone morphogenetic protein-4 and trans-retinoic acid.
[Scientific Reports]
Derivation of oocyte-like cells from putative embryonic stem cells and parthenogenetically activated into blastocysts in goat | Scientific Reports. (n.d.). Retrieved June 22, 2020, from https://www.nature.com/articles/s41598-020-66609-2 Cite
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Epigenetic Priming by Dppa2 and 4 in Pluripotency Facilitates Multi-Lineage Commitment

Differentiation assays revealed that developmental pluripotency associated 2 and 4 (Dppa2/4) double knockout mouse ESCs failed to exit pluripotency and differentiate efficiently.
[Nature Structural & Molecular Biology]
Eckersley-Maslin, M. A., Parry, A., Blotenburg, M., Krueger, C., Ito, Y., Franklin, V. N. R., Narita, M., D’Santos, C. S., & Reik, W. (2020). Epigenetic priming by Dppa2 and 4 in pluripotency facilitates multi-lineage commitment. Nature Structural & Molecular Biology, 1–10. https://doi.org/10.1038/s41594-020-0443-3 Cite
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In Vitro Differentiated Human Stem Cell-Derived Neurons Reproduce Synaptic Synchronicity Arising during Neurodevelopment

Scientists generated human iPSC-derived neurons exhibiting spontaneous oscillatory activity after cultivation of up to six months, which resembled early oscillations observed in rodent neurons. This behavior was found in neurons generated using a more “native” embryoid body protocol, in contrast to a “fast” protocol based on NGN2 overexpression.
[Stem Cell Reports]
Rosa, F., Dhingra, A., Uysal, B., Mendis, G. D. C., Loeffler, H., Elsen, G., Mueller, S., Schwarz, N., Castillo-Lizardo, M., Cuddy, C., Becker, F., Heutink, P., Reid, C. A., Petrou, S., Lerche, H., & Maljevic, S. (2020). In Vitro Differentiated Human Stem Cell-Derived Neurons Reproduce Synaptic Synchronicity Arising during Neurodevelopment. Stem Cell Reports, 0(0). https://doi.org/10.1016/j.stemcr.2020.05.015 Cite
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A Simple, Quick, and Efficient CRISPR/Cas9 Genome Editing Method for Human Induced Pluripotent Stem Cells

The authors report an electroporation-mediated plasmid CRISPR/Cas9 delivery approach for genome editing in iPSCs. With this approach, an edited iPSC cell line could be obtained within two weeks.
[Acta Pharmacologica Sinica]
Geng, B., Choi, K.-H., Wang, S., Chen, P., Pan, X., Dong, N., Ko, J.-K., & Zhu, H. (2020). A simple, quick, and efficient CRISPR/Cas9 genome editing method for human induced pluripotent stem cells. Acta Pharmacologica Sinica, 1–6. https://doi.org/10.1038/s41401-020-0452-0 Cite
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BRPF3-HUWE1-Mediated Regulation of MYST2 Is Required for Differentiation and Cell-Cycle Progression in Embryonic Stem Cells

Researchers provided novel insights into understanding the functions of Brpf3 in proper differentiation as well as cell-cycle progression of ESCs via regulation of Myst2 stability by obstructing Huwe1-mediated ubiquitination.
[Cell Death & Differentiation]
Cho, H. I., Kim, M. S., Lee, J., Yoo, B. C., Kim, K. H., Choe, K.-M., & Jang, Y. K. (2020). BRPF3-HUWE1-mediated regulation of MYST2 is required for differentiation and cell-cycle progression in embryonic stem cells. Cell Death & Differentiation, 1–16. https://doi.org/10.1038/s41418-020-0577-1 Cite
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Tooth Formation: Are the Hardest Tissues of the Human Body Hard to Regenerate?

Approaches for whole-tooth regeneration utilizing adult stem cells, iPSCs, or tooth germ cells transplantation are emerging as promising alternatives to overcome existing in vitro tissue generation hurdles. The authors discuss the most recent advances in cellular signaling guiding dental tissue genesis.
[International Journal of Molecular Sciences]
Baranova, J., Büchner, D., Götz, W., Schulze, M., & Tobiasch, E. (2020). Tooth Formation: Are the Hardest Tissues of Human Body Hard to Regenerate? International Journal of Molecular Sciences, 21(11), 4031. https://doi.org/10.3390/ijms21114031 Cite
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AgeX Therapeutics and Pluristyx Announce Manufacturing, Marketing, and Distribution Agreement to Expand Access to Clinical-Grade Human Pluripotent Stem Cells for Therapeutic Applications

AgeX Therapeutics, Inc. and Pluristyx, Inc. have announced they have entered into a manufacturing, marketing, and distribution agreement through which Pluristyx will undertake these activities on behalf of AgeX with respect to AgeX’s research- and clinical-grade ESI brand human ESCs.
[AgeX Therapeutics Inc.]
AgeX Therapeutics | single-news-story. (n.d.). Retrieved June 17, 2020, from https://www.agexinc.com/investors-media/single-news-story/ Cite
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Autism-Associated SHANK3 Mutations Impair Maturation of Neuromuscular Junctions and Striated Muscles

Investigators used a combination of patient-derived human iPSCs, Shank3Δ11(−/−) mice, and Phelan-McDermid syndrome muscle biopsies from patients of different ages to analyze the role of SHANK3 on motor unit development.
[Science Translational Medicine]
Autism-associated SHANK3 mutations impair maturation of neuromuscular junctions and striated muscles | Science Translational Medicine. (n.d.). Retrieved June 17, 2020, from https://stm.sciencemag.org/content/12/547/eaaz3267 Cite
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High-Throughput Intracellular Biopsy of microRNAs for Dissecting the Temporal Dynamics of Cellular Heterogeneity

Using intracellular biopsy technique (inCell-Biopsy), scientists analyzed the temporal miRNA transcriptome over the differentiation of ESCs. The combinatorial miRNA expression patterns derived by inCell-Biopsy identified emerging cell subpopulations differentiated from ESCs and revealed the dynamic evolution of cellular heterogeneity.
[Science Advances]
Wang, Z., Qi, L., Yang, Y., Lu, M., Xie, K., Zhao, X., Cheung, E. H. C., Wang, Y., Jiang, X., Zhang, W., Huang, L., Wang, X., & Shi, P. (2020). High-throughput intracellular biopsy of microRNAs for dissecting the temporal dynamics of cellular heterogeneity. Science Advances, 6(24), eaba4971. https://doi.org/10.1126/sciadv.aba4971 Cite
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A lncRNA-miRNA-mRNA Network for Human Primed, Naive and Extended Pluripotent Stem Cells

Researchers successfully converted three in-house-derived primed human PSC lines (H10, H24, and iPS) to a naive state and an expanded pluripotent stem cell (EPS) state. Primed, naive and EPS cells displayed state-specific morphologies and expressed pluripotent markers.
[PLoS One]
Ma, Z., Li, Y., Zhang, Y., Chen, J., Tan, T., & Fan, Y. (2020). A lncRNA-miRNA-mRNA network for human primed, naive and extended pluripotent stem cells. PLOS ONE, 15(6), e0234628. https://doi.org/10.1371/journal.pone.0234628 Cite
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