Oscillating Calcium Signals in Smooth Muscle Cells Underlie the Persistent Basal Tone of Internal Anal Sphincter

Scientists report the origin and molecular mechanisms of calcium signals that control the internal anal sphincter (IAS) basal tone, using a combination approach including a novel IAS slice preparation that retains cell arrangement and architecture as in vivo, 2‐photon imaging, and cell‐specific gene‐modified mice.
[Journal of Cellular Physiology]
Lu, P., Chen, J., Zhang, C., Saur, D., Baer, C. E., Lifshitz, L. M., Fogarty, K. E., & ZhuGe, R. (n.d.). Oscillating calcium signals in smooth muscle cells underlie the persistent basal tone of internal anal sphincter. Journal of Cellular Physiology, n/a(n/a). https://doi.org/https://doi.org/10.1002/jcp.30279 Cite
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Epigenetic Regulation of Satellite Cell Fate during Skeletal Muscle Regeneration

The authors describe the current knowledge of the mechanisms allowing muscle stem cell fate transitions during regeneration and how it is altered in muscle disease.
[Skeletal Muscle]
Massenet, J., Gardner, E., Chazaud, B., & Dilworth, F. J. (2021). Epigenetic regulation of satellite cell fate during skeletal muscle regeneration. Skeletal Muscle, 11(1), 4. https://doi.org/10.1186/s13395-020-00259-w Cite
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Sarepta Therapeutics and Genevant Sciences Announce Research Collaboration for Lipid Nanoparticle-Based Gene Editing Therapeutics

Sarepta Therapeutics, Inc. and Genevant Sciences announced a research collaboration and option agreement for the delivery of LNP-gene editing therapeutics in Sarepta’s pipeline for neuromuscular diseases.
[Sarepta Therapeutics, Inc.]
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Myocyte-Specific Enhancer Factor 2c Triggers Transdifferentiation of Adipose Tissue-Derived Stromal Cells into Spontaneously Beating Cardiomyocyte-Like Cells

Investigators developed a simple culture protocol under which the adult murine inguinal adipose tissue-derived stromal vascular fraction (Ad-SVF) reproducibly transdifferentiates into beating cardiomyocyte (CMs) without induction. The beating CMs showed the striated ventricular phenotype of cardiomyocytes and synchronised oscillation of the intracellular calcium concentration among cells on day 28 of Ad-SVF primary culture.
[Scientific Reports]
Takashima, S., Usui, S., Inoue, O., Goten, C., Yamaguchi, K., Takeda, Y., Cui, S., Sakai, Y., Hayashi, K., Sakata, K., Kawashiri, M., & Takamura, M. (2021). Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells. Scientific Reports, 11(1), 1520. https://doi.org/10.1038/s41598-020-80848-3 Cite
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MicroRNA-494-3p Inhibits Formation of Fast Oxidative Muscle Fibres by Targeting E1A-Binding Protein p300 in Human-Induced Pluripotent Stem Cells

Myogenesis in human induced pluripotent stem cells with the Tet/ON-myogenic differentiation 1 gene was induced by culturing them in doxycycline-supplemented differentiation medium for seven days.
[Scientific Reports]
Iwasaki, H., Ichihara, Y., Morino, K., Lemecha, M., Sugawara, L., Sawano, T., Miake, J., Sakurai, H., Nishi, E., Maegawa, H., & Imamura, T. (2021). MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells. Scientific Reports, 11(1), 1161. https://doi.org/10.1038/s41598-020-80742-y Cite
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A Comprehensive Epigenome Atlas Reveals DNA Methylation Regulating Skeletal Muscle Development

The authors generated the first single-base resolution DNA methylome and transcriptome maps of porcine skeletal muscle across 27 developmental stages.
[Nucleic Acids Research]
Yang, Y., Fan, X., Yan, J., Chen, M., Zhu, M., Tang, Y., Liu, S., & Tang, Z. (2021). A comprehensive epigenome atlas reveals DNA methylation regulating skeletal muscle development. Nucleic Acids Research, gkaa1203. https://doi.org/10.1093/nar/gkaa1203 Cite
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Deep Muscle-Proteomic Analysis of Freeze-Dried Human Muscle Biopsies Reveals Fiber Type-Specific Adaptations to Exercise Training

Researchers developed a workflow enabling proteomics analysis of pools of isolated muscle fibers from freeze-dried human muscle biopsies.
[Nature Communications]
Deshmukh, A. S., Steenberg, D. E., Hostrup, M., Birk, J. B., Larsen, J. K., Santos, A., Kjøbsted, R., Hingst, J. R., Schéele, C. C., Murgia, M., Kiens, B., Richter, E. A., Mann, M., & Wojtaszewski, J. F. P. (2021). Deep muscle-proteomic analysis of freeze-dried human muscle biopsies reveals fiber type-specific adaptations to exercise training. Nature Communications, 12(1), 304. https://doi.org/10.1038/s41467-020-20556-8 Cite
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Uremic Toxins Impair Skeletal Muscle Regeneration by Inhibiting Myoblast Proliferation, Reducing Myogenic Differentiation, and Promoting Muscular Fibrosis

This study analyzes the role of uremic toxins in sarcopenia at different stages of chronic kidney disease (CKD), evaluating changes in the muscular regeneration process. Cultured C2C12 cells were incubated with a combination of indoxyl sulphate and p-cresol at high doses or low doses resembling late or early CKD stages, respectively.
[Scientific Reports]
Alcalde-Estévez, E., Sosa, P., Asenjo-Bueno, A., Plaza, P., Olmos, G., Naves-Díaz, M., Rodríguez-Puyol, D., López-Ongil, S., & Ruiz-Torres, M. P. (2021). Uraemic toxins impair skeletal muscle regeneration by inhibiting myoblast proliferation, reducing myogenic differentiation, and promoting muscular fibrosis. Scientific Reports, 11(1), 512. https://doi.org/10.1038/s41598-020-79186-1 Cite
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Endothelial Cells Control Vascular Smooth Muscle Cell Cholesterol Levels by Regulating 24-Dehydrocholesterol Reductase Expression

The authors investigated changes of the vascular smooth muscle cell (VSMC) transcriptome by utilizing 3D human vascular organoids organized as a core of VSMCs enclosed by a monolayer of endothelial cells.
[Experimental Cell Research]
Kohlhaas, J., Jäger, M., Lust, L., De La Torre, C., Hecker, M., & Korff, T. (2021). Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression. Experimental Cell Research, 112446. https://doi.org/10.1016/j.yexcr.2020.112446 Cite
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Prilenia Announces First Patient Enrolled for Pridopidine Phase II/III Platform Trial for ALS

Prilenia Therapeutics B.V. announced the enrollment of the first participant for treatment with pridopidine in the pivotal HEALEY ALS Platform Trial.
[Prilenia Therapeutics B.V.]
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Pfizer Doses First Participant in Phase III Study for Duchenne Muscular Dystrophy Investigational Gene Therapy

Pfizer Inc. announced that the first participant has been dosed in the Phase III CIFFREO study, which will evaluate the efficacy and safety of investigational gene therapy candidate PF-06939926 in boys with Duchenne muscular dystrophy.
[Pfizer Inc.]
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