Investigators showed that tumor development could be simply induced independently of intestinal microenvironment, even with WT murine primary intestinal cells alone. Consistent with cellular transformation, tumor-derived epithelial cells acquired sphere formation potential, gave rise to secondary tumors on retransplantation, and highly expressed cancer stem cell markers. [Proc Natl Acad Sci USA] Abstract Aptamer Identification of Brain Tumor Initiating Cells
To develop reagents that enrich for tumor initiating cells (TICs) but not matched non-TICs or tissue-specific stem cells, researchers adopted Cell-Systematic Evolution of Ligands by Exponential Enrichment (Cell-SELEX) to identify glioblastomas TIC-specific nucleic acid probes – aptamers – that specifically bind TICs. In this study, using Cell-SELEX with positive selection for TICs and negative selection for non-TICs and human neural progenitor cells the authors identified TIC aptamers that specifically bind to TICs with excellent Kds. [Cancer Res] Abstract
Clinical Relevance of Cancer Stem Cells in Bone Marrow of Early Breast Cancer Patients
Researchers hypothesized that if cancer stem cells are responsible for tumor dissemination, their presence in bone marrow would be prognostic in early stages of breast cancer patients. [Ann Oncol] Abstract
Infiltrating Bone Marrow Mesenchymal Stem Cells Increase Prostate Cancer Stem Cell Population and Metastatic Ability via Secreting Cytokines to Suppress Androgen Receptor Signaling
Scientists showed that prostate cancer (PCa) cells could recruit bone marrow mesenchymal stem cells and consequently the metastatic ability of PCa cells was increased. They also found that the increased metastatic ability of PCa cells could be due to the increased PCa stem cell population. [Oncogene] Abstract
Primary Breast Cancer Stem-Like Cells Metastasize to Bone, Switch Phenotype and Acquire a Bone Tropism Signature
Investigators used a human-in-mice model to study bone metastasis formation due to primary breast cancer stem cells-like colonization. [Br J Cancer] Abstract
ALDH1 Expression Is Enriched in Breast Cancers Arising in Young Women but Does Not Predict Outcome
The authors used ALDH1 as a cancer stem cell marker and determined whether ALDH1 expression correlated with clinical outcome in young women with breast cancer. [Br J Cancer] Abstract
Differential Proteomic Analysis of Cancer Stem Cell Properties in Hepatocellular Carcinomas by Isobaric Tag Labeling and Mass Spectrometry
Although a number of molecules have been identified in different cancer cells as markers for cancer stem cells, no promising markers are currently available for hepatocellular carcinoma cells. In this study, two clones of Hep3B cell lines were functionally characterized as control or cancer stem cell-like cells, based on properties including spheroid formation, drug resistance, and tumor initiation. [J Proteome Res] Abstract
Isolation and Characterization of Human Breast Cancer Cells with SOX2 Promoter Activity
Researchers developed a reporter system using fluorescent protein driven by the promoter for sex-determining region Y-box 2 (SOX2) gene to detect and isolate living SOX2-positive cells. Results suggest that the cell population with SOX2 promoter activity contains cancer stem cell (CSC)-like cells which show expression profiles different from those of CSC-marker genes previously recognized in human breast cancers. [Biochem Biophys Res Commun] Abstract