Preclinical Evaluation of Echinomycin: Therapeutic Efficacy in Relapsed Acute Myeloid Leukemia without Adverse Effects on Host Hematopoietic Stem Cells In mice with complete remission, Echinomycin appeared to completely eliminate leukemia initiating cells as no leukemia could be propagated in vivo following serial transplantation. [Blood] Abstract Sequencing of Breast Cancer Stem Cell Populations Indicates a Dynamic Conversion between Differentiation States In Vivo Researchers undertook exome sequencing of cancer stem-like cells (CSCs) from 12 breast cancer patients, along with paired primary tumor samples. Their analysis revealed that the majority of somatic mutations are shared between CSCs and bulk primary tumors, with similar frequencies in the two. [Breast Cancer Res] Abstract | Full Article Novel Role of Pancreatic Differentiation 2 in Facilitating Self-Renewal and Drug Resistance of Pancreatic Cancer Stem Cells Knockdown of pancreatic differentiation 2 in cancer stem cells resulted in reduced viability of cells and enhanced apoptosis along with abrogated expression of CD133 and MDR2. [Br J Cancer] Abstract Viral Oncogene Expression in the Stem/Progenitor Cell Compartment of the Mouse Intestine Induces Adenomatous Polyps Expression of a truncated SV40 large T-antigen in murine intestinal crypts promoted the formation of numerous adenomatous polyps in the colon and small intestine. In contrast, when the same T-antigen construct is expressed in villous enterocytes the consequences are limited to hyperplasia and dysplasia. [Mol Cancer Res] Abstract Reversing Paclitaxel Resistance in Ovarian Cancer Cells via Inhibition of the ABCB1 Expressing Side Population Researchers demonstrate that side population (SP) cells can be detected in both ovarian cancer cell lines and ascitic fluid samples, and these SP cells possess stem cell and drug resistance properties. [Tumor Biol] Abstract Upregulation of Autophagy by Hypoxia-Inducible Factor-1α Promotes EMT and Metastatic Ability of CD133+ Pancreatic Cancer Stem-Like Cells during Intermittent Hypoxia Researchers examined if intermittent hypoxia facilitates the invasiveness of human pancreatic cancer cell lines (Panc-1 and BxPC-3) by Transwell assay. They used western blotting and flow cytometry analysis to quantify stem-like cells in the migratory cells during intermittent hypoxia in the human pancreatic cancer cells. [Oncol Rep] Abstract Retroviral-Infection Increases Tumorigenic Potential of MDA-MB-231 Breast Carcinoma Cells by Expanding an Aldehyde Dehydrogenase (ALDH1) Positive Stem-Cell Like Population Scientists demonstrate that transduction of human breast carcinoma cells (MDA-MB231) with LXSN and QCXIP retroviral vectors causes significant increases in growth rate, clonogenic fraction, and aldehyde dehydrogenase-1 positive cells, which is associated with increased steady-state levels of cancer stem cell populations. [Redox Biol] Full Article Growth Arrest and Forced Differentiation of Human Primary Glioblastoma Multiforme by a Novel Small Molecule Researchers show that the treatment of the novel small molecule, CG500354, into CD133-expressing human primary GBM cells induces growth arrest by cell cycle regulators, p53, p21, p27 and phase-specific cyclins, and neural differentiation, as confirmed by neural progenitor/precursor markers, nestin, GFAP and Tuj1. [Sci Rep] Full Article |