KAP1 Promotes Proliferation and Metastatic Progression of Breast Cancer Cells Scientists report that the expression of many KRAB family zinc finger proteins along with active SUMOlyated KAP1 is elevated widely in human breast cancers. KAP1 silencing in breast cancer cells reduced proliferation and inhibited the growth and metastasis of tumor xenografts. [Cancer Res] Abstract Mitochondria as New Therapeutic Targets for Eradicating Cancer Stem Cells: Quantitative Proteomics and Functional Validation via MCT1/2 Inhibition Researchers used quantitative proteomics analysis to identify novel therapeutic targets in cancer stem cells and/or progenitor cells. For this purpose, mammospheres from two ER-positive breast cancer cell lines were grown in suspension using low-attachment plates and directly compared to attached monolayer cells grown in parallel. [Oncotarget] Full Article | Press Release Systematic Identification of Single Amino Acid Variants in Glioma Stem-Cell-Derived Chromosome 19 Proteins Novel proteoforms with single amino acid variations represent proteins that often have altered biological functions but are less explored in the human proteome. Scientists developed an approach, searching high quality shotgun proteomic data against an extended protein database, to identify expressed mutant proteoforms in glioma stem cell lines. [J Proteome Res] Abstract | Graphical Abstract Active Targeting Docetaxel-PLA Nanoparticles Eradicate the Circulating Lung Cancer Stem Like Cells and Inhibit the Liver Metastasis Researchers aimed to establish a cancer stem cells (CSCs)-targeting polylactic acid encapsulated docetaxel nanoparticles for anti-metastatic therapy. Cyclic binding peptides were screened on CSCs in vitro and the peptide CVKTPAQSC exhibiting high specific binding ability to pulmonary adenocarcinoma tissue was subsequently conjugated to the nanoparticles loaded with docetaxel. [Mol Pharm] Abstract Nitric Oxide Induces Cancer Stem Cell-Like Phenotypes in Human Lung Cancer Cells Researchers show how nitric oxide, a key cellular modulator whose level is elevated in many tumors, affects cancer stem cell-like phenotypes of human non-small-cell lung carcinoma H292 and H460 cells. [Am J Physiol Cell Physiol] Abstract TRIM28 and β-Actin Identified via Nanobody-Based Reverse Proteomics Approach as Possible Human Glioblastoma Biomarkers Scientists immunized an alpaca with a human glioblastoma multiforme (GBM) stem-like cell line prepared from primary GBM cultures. Next, a nanobody library was constructed in a phage-display vector. Using nanobody phage-display technology they selected specific GBM stem-like cell binders through a number of affinity selections. [PLoS One] Full Article Overexpression of miR-200a Suppresses Epithelial-Mesenchymal Transition of Liver Cancer Stem Cells Researchers investigated the role of miR-200a in regulation of epithelial-mesenchymal transition phenotype of liver cancer stem cells (LCSCs). They used side population (SP) sorting to obtain cancer stem-like cells from hepatocellular carcinoma cell lines and identified that the SP fraction could be enriched with LCSCs. [Tumour Biol] Abstract Prognostic Relevance of Carbonic Anhydrase IX Expression Is Distinct in Various Subtypes of Breast Cancer and Its Silencing Suppresses Self-Renewal Capacity of Breast Cancer Cells Scientists elucidated the prognostic significance of carbonic anhydrase IX (CAIX) in the intrinsic subtypes of breast cancer and to characterize CAIX as a drug target in breast cancer. [Cancer Chemother Pharmacol] Abstract |