Notch Reporter Activity in Breast Cancer Cell Lines Identifies a Subset of Cells with Stem Cell Activity Investigators utilized a lentiviral Notch reporter system to identify a subset of cells with a higher Notch activity or reduced activity in multiple breast cancer cell lines. Using in vitro and mouse xenotransplantation assays, they investigated the role of the Notch pathway in breast cancer stem cell regulation. [Mol Cancer Ther] Abstract p21-Activated Kinase 1 Determines Stem-Like Phenotype and Sunitinib Resistance via NF-κB/IL-6 Activation in Renal Cell Carcinoma p21-Activated Kinase 1 (PAK1) kinase activation with constitutive active PAK1 mutant T423E promoted growth, colony formation, migration, invasion and stem-like phenotype of renal cell carcinoma cells, and vice versa, in PAK1 inhibition by PAK1 kinase inactivation with specific PAK1 shRNA, dead kinase PAK1 mutant K299R or allosteric inhibitor IPA3. [Cell Death Dis] Full Article Establishment of a Novel System for the Culture and Expansion of Hepatic Stem-Like Cancer Cells Scientists established a novel medium system that allows the culture and enrichment of hepatic stem-like cancer cells from both hepatoma cells and human primary hepatocellular carcinoma cells. These cells exhibited typical stem cell properties, such as enhanced stem cell markers, gain of epithelial-mesenchymal transition properties and drug resistance, and more importantly, stronger tumor-initiating capabilities. [Cancer Lett] Full Article Targeting Lung Cancer Stem-Like Cells with TRAIL Gene Armed Oncolytic Adenovirus Lung cancer cell line A549 cells propagated as spheroid bodies in growth factors-defined serum-free medium. A549 sphere cells displayed cancer stem cell properties, including chemo-resistance, increased proportion of G0/G1 cells, slower proliferation rate, ability of differentiation and enhanced tumor formation ability in vivo. [J Cell Mol Med] Full Article Evidence of CD90+CXCR4+ Cells as Circulating Tumor Stem Cells in Hepatocellular Carcinoma Scientists show evidence that CD90+CXCR4+ hepatocellular carcinoma (HCC) cells may be circulating tumor stem cells in HCC. CD90+CXCR4+ HCC cells formed tumor spheres in culture and developed tumors after serial adoptive transplantations into NOD/SCID mice, while the CD90–CXCR4–, CD90–CXCR4+ or CD90+CXCR4− cells did not. [Tumor Biol] Abstract Synergistic Anti-Proliferative Effect of Metformin and Sorafenib on Growth of Anaplastic Thyroid Cancer Cells and Their Stem Cells In HTh74 anaplastic thyroid carcinoma cells and its derived doxorubicin-resistant HTh74Rdox cell line, the growth inhibitory effect of sorafenib with or without metformin was investigated. The two drugs synergistically decreased sphere formation, which suggested a specific effect on thyroid cancer stem cells. [Oncol Rep] Abstract IQGAP1 Is Involved in Enhanced Aggressive Behavior of Epithelial Ovarian Cancer Stem Cell-Like Cells during Differentiation Ovarian cancer stem cell-like cells (CSC-LCs) obtained through spheroid culture showed irregularly elongated appearance, and were CD24 negative and OCT4 and SOX2 positive. IQ domain GTPase-activating protein 1 (IQGAP1) expression was decreased in ovarian CSC-LCs compared with parental 3AO cells, but increased de novo during the differentiation of CSC-LCs. [Int J Gynecol Cancer] Full Article Ovarian Cancer Stem-Like Cells Elicit the Polarization of M2 Macrophages Increasing evidence has supported the correlation between ovarian cancer stem-like cells (OCSCs) and macrophages, however, whether OCSCs can affect the polarization of macrophages and the underlying mechanisms involved remain to be elucidated. To examine the interplay between OCSCs and macrophages, a co‑culture system was used to detect the effect of OCSCs on macrophage polarization [Mol Med Rep] Abstract |