Effect of Transendocardial Delivery of Autologous Bone Marrow Mononuclear Cells on Functional Capacity, Left Ventricular Function, and Perfusion in Chronic Heart Failure Researchers’ objectives were to determine if administration of bone marrow mononuclear cells through transendocardial injections improves myocardial perfusion, reduces left ventricular end-systolic volume, or enhances maximal oxygen consumption in patients with coronary artery disease or left ventricular dysfunction, and limiting heart failure or angina. [JAMA] Abstract | Press Release Direct Conversion of Fibroblasts into Stably Expandable Neural Stem Cells Researchers show direct derivation of stably expandable neural stem cells from mouse fibroblasts through a curtailed version of reprogramming to pluripotency. [Cell Stem Cell] Abstract | Press Release Direct Reprogramming of Fibroblasts into Neural Stem Cells by Defined Factors Investigators show that a combination of transcription factors induces mouse fibroblasts to directly acquire a neural stem cell identity which they term as induced neural stem cells. [Cell Stem Cell] Abstract | Press Release Ubiquitous High-Level Gene Expression in Hematopoietic Lineages Provides Effective Lentiviral Gene Therapy of Murine Wiskott-Aldrich Syndrome Transplantation of stem cells transduced with MND-human Wiskott-Aldrich Syndrome protein (WASp) lentiviral vector resulted in sustained, endogenous levels of WASp in all hematopoietic lineages, progressive selection for WASp+ T, NKT and B cells, rescue of T cell proliferation and cytokine production, and substantial restoration of marginal zone B cells. [Blood] Abstract Co-Transplantation of Pure Blood Stem Cells with Antigen-Specific but Not Bulk T Cells Augments Functional Immunity Researchers studied reactivity against murine CMV in lethally irradiated mice given allogeneic purified hematopoietic stem cells (HSCs) or HSCs supplemented with T cells or T-cell subsets. [Proc Natl Acad Sci USA] Abstract Pluripotent Stem Cell-Engineered Cell Sheets Re-Assembled with Defined Cardiovascular Populations Ameliorate Reduction in Infarct Heart Function Through Cardiomyocyte-Mediated Neovascularization Investigators show the potentials and mechanisms of cardiac tissue sheet transplantation in cardiac function after myocardial infarction. [Stem Cells] Abstract CNS-Specific Therapy for Ongoing EAE by Silencing Interleukin-17 Pathway in Astrocytes Scientists report that intracerebroventricular injection of a novel lentiviral vector to knockdown Act1 expression in astrocytes effectively inhibited disease progression at experimental autoimmune encephalomyelitis (EAE) induction, clinical onset, and peak of disease, with significantly reduced numbers of infiltrating inflammatory cells and percentage of Th17 cells in the central nervous system (CNS). [Mol Ther] Abstract Off-the-Shelf Adenoviral-Mediated Immunotherapy via Bicistronic Expression of Tumor Antigen and iMyD88/CD40 Adjuvant Researchers demonstrate that simple tumor-specific antigen delivery via adenoviral vectors results in strong antitumor immunity. [Mol Ther] Abstract Targeting the Epidermal Growth Factor Receptor in Non-Small Cell Lung Cancer Cells: The Effect of Combining RNA Interference with Tyrosine Kinase Inhibitors or Cetuximab Researchers have examined, in different non-small cell lung cancer cell lines, the combined effect of RNA interference targeting the epidermal growth factor receptor (EGFR) mRNA, and inactivation of EGFR signaling using different receptor tyrosine kinase inhibitors or a monoclonal antibody cetuximab. [BMC Med] Abstract | Press Release Fertile Offspring Derived from Mouse Spermatogonial Stem Cells Cryopreserved for More than 14 Years Researchers demonstrate that long-term cryopreservation of testis cells from mouse, rat, rabbit and baboon safeguards spermatogonial stem cell viability, and that these cells can colonize the seminiferous tubules of recipient testes. [Hum Reprod] Abstract Optimized Adoptive T-Cell Therapy for the Treatment of Residual Mantle Cell Lymphoma Researchers demonstrated that dendritic cells primed with mantle cell lymphoma (MCL) antigens stimulated the activation of MCL-specific T cells that recognized and destroyed both MCL cell lines and primary MCL in vitro. [Cancer Immunol Immunother] Abstract Structured Three-Dimensional Co-Culture of Mesenchymal Stem Cells with Meniscus Cells Promotes Meniscal Phenotype without Hypertrophy Scientists tested the feasibility of using mesenchymal stem cells co-cultured with mature meniscal cells for meniscus tissue engineering. [Biotechnol Bioeng] Abstract Differentiated Adipose-Derived Stem Cells Promote Myelination and Enhance Functional Recovery in a Rat Model of Chronic Denervation Rat adipose-derived stem cells transdifferentiated into a Schwann cell phenotype cocultured with dorsal root ganglion neurons were shown to associate with neurites and to express myelin basic protein-positive myelin protein. [J Neurosci Res] Abstract
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