A Novel Therapeutic Cytomegalovirus DNA Vaccine in Allogeneic Hematopoietic Stem-Cell Transplantation: A Randomized, Double-Blind, Placebo-Controlled, Phase II Trial To reduce the potential for disease and subsequent need for antiviral drugs, researchers aimed to assess safety and efficacy of a cytomegalovirus therapeutic DNA vaccine compared with placebo. [Lancet Infect Dis] Abstract | Press Release Reversal of Type 1 Diabetes via Islet Beta Cell Regeneration following Immune Modulation by Cord Blood-Derived Multipotent Stem Cells Researchers developed a procedure for Stem Cell Educator therapy in which a patient’s blood is circulated through a closed-loop system that separates lymphocytes from the whole blood and briefly co-cultures them with adherent cord blood-derived multipotent stem cells before returning them to the patient’s circulation. [BMC Med] Abstract | Press Release Bmi1 Is Critical to Prevent Ikaros-Mediated Lymphoid Priming in Hematopoietic Stem Cells Researchers’ results indicate that Ikaros is a critical Bmi1 target in vivo that prevents premature lineage specification of hematopoietic stem cells. [Cell Cycle] Abstract Omission of In Vivo T-Cell Depletion Promotes Rapid Expansion of Naïve CD4+ Cord Blood Lymphocytes and Restores Adaptive Immunity within 2 Months after Unrelated Cord Blood Transplant Researchers studied the pattern of early immune reconstitution and the clinical outcome of children undergoing unrelated umbilical cord blood transplant when in vivo T-cell depletion was omitted. [Br J Haematol] Abstract Erythroid-Specific Expression of β-Globin from Sleeping Beauty-Transduced Human Hematopoietic Progenitor Cells In this study scientists utilized the non-viral Sleeping Beauty (SB) transposon system using the SB100X hyperactive transposase to transduce human cord blood CD34+ cells with DsRed and a hybrid IHK–β-globin transgene. [PLoS One] Abstract Co-Transplantation of Ex Vivo Expanded and Unexpanded Cord Blood Units in Immunodeficient Mice Using IGFBP2-Augmented Mesenchymal Cell Co-Cultures Here investigators demonstrate an immunodeficient mouse model for co-transplantation of ex vivo expanded and unexpanded human cord blood (CB), showing enhanced CB engraftment and provide proof of concept for this transplantation strategy as a means of overcoming the limiting cell numbers in each CB unit. [Biol Blood Marrow Transplant] Abstract In Vitro Evaluation of Graft-Versus-Graft Alloreactivity as a Tool to Identify the Predominant Cord Blood Unit Prior to Double Cord Blood Transplantation Researchers investigated the mechanisms underlying single-donor predominance in double-cord blood transplantation by studying in vitro the role of the graft-versus-graft cell-mediated immune effect in two-way mixed lymphocyte culture, and the contribution of differential hematopoietic progenitor (HP)-potency in HP mixed-cultures. [Biol Blood Marrow Transplant] Abstract Human Umbilical Cord Wharton’s Jelly Mesenchymal Stem Cells Do Not Transform to Tumor-Associated Fibroblasts in the Presence of Breast and Ovarian Cancer Cells Unlike Bone Marrow Mesenchymal Stem Cells The common origin of the Wharton’s jelly mesenchymal stem cells (hWJMSCs) and the finally homed human bone marrow MSCs prompted researchers to evaluate whether hWJSCs are also involved in tumor-associated fibroblast transformation. [J Cell Biochem] Abstract |