Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF Researchers demonstrated a novel myocyte differentiation protocol that can yield a significant, high percentage of cardiac myocyte differentiation (>85%) in two human induced pluripotent stem cells (hiPSCs) lines, which makes the fabrication of a human cardiac muscle patch possible. The established hiPSCs cell lines being examined include the transgene integrated UCBiPS7 derived from cord blood cells and non-integrated PCBC16iPS from skin fibroblasts. [PLoS One] Full Article Bone Morphogenetic Protein-9 Activates Smad and ERK Pathways and Supports Human Osteoclast Function and Survival In Vitro To determine the effects of BMP-9 on osteoclast formation, activity and survival, investigators used human cord blood monocytes as osteoclast precursors that form multinucleated osteoclasts in the presence of RANKL and M-CSF in long-term cultures. [Cell Signal] Abstract Human Wharton’s Jelly Stem Cell Conditioned Medium Enhances Freeze-Thaw Survival and Expansion of Cryopreserved CD34+ Cells Various approaches are being undertaken to expand hematopoietic stem cells ex vivo from single cord blood units. Researchers report a protocol involving slow freezing (-1 °C per minute to -120 °C) + freezing medium containing DMSO + FBS + 24 h-50 % hWJSC-CM that enhances thaw-survival of CD34+ cells. [Stem Cell Rev] Abstract Comparison of Human Mesenchymal Stem Cells Derived from Adipose and Cord Tissue Although bone marrow-derived mesenchymal stem cells (MSCs) have multi-lineage differentiation potential, bone marrow is not an optimal source because of the isolation process and low yield. Researchers investigated comparatively for the first time the in vitro regenerative potential of human MSCs from two other sources: umbilical cord tissue and adipose tissue. [Cytotherapy] Abstract Viability of Umbilical Cord Blood Mononuclear Cell Subsets until 96 Hours after Collection Investigators evaluated the viability of several umbilical cord blood cell subsets until 96 hours after collection, to examine whether this delay would be acceptable for processing and freezing the samples. [Transfusion] Abstract Characterization of Various Blood and Graft Sources: A Prospective Series Researchers prospectively compared by fluorescence-activated cell sorting analyses the cellular composition of three blood sources on the one hand, peripheral blood (PB) versus granulocyte-colony-stimulating factor-mobilized PB versus cord blood (CB), and of three graft sources on the other hand, unmanipulated bone marrow versus leukapheresis product versus thawed CB graft. [Transfusion] Abstract Acellular Spinal Cord Scaffold Seeded with Mesenchymal Stem Cells Promotes Long-Distance Axon Regeneration and Functional Recovery in Spinal Cord Injured Rats Scientists investigated whether the spinal cord lesion gap could be bridged by implantation of bionic-designed acellular spinal cord scaffold alone and seeded with human umbilical cord blood-derived mesenchymal stem cells respectively, and their effects on functional improvement. [J Neurol Sci] Abstract Long-Term Outcome of Human Cord Blood-Derived Hematopoietic Progenitor Cells in Murine Lungs Intranasally delivered human cord blood-derived CD34+ hematopoietic progenitor cells have the capacity to engraft and undergo transdifferentiation to surfactant-containing alveolar epithelial type II cell-like cells in lungs of newborn mice. Researchers aimed to determine the long-term fate of such transplanted cells as well as their effects on alveolar development in neonatally injured lungs. [Exp Lung Res] Abstract |