Late Outgrowth Endothelial Cells Resemble Mature Endothelial Cells and Are Not Derived from Bone Marrow Researchers aimed to identify the source and precursor of endothelial outgrowth cells (EOC) and to assess their regenerative potential compared to mature endothelial cells. EOC are readily isolated from umbilical cord blood (6/6 donors) and peripheral blood mononuclear cells (4/6 donors) but not from bone marrow (0/6) or peripheral blood following mobilization with granulocyte-colony stimulating factor (0/6 donors). [Stem Cells] Abstract Human Neonatal Naive CD4+ T Cells Have Enhanced Activation-Dependent Signaling Regulated by the MicroRNA miR-181a Scientists investigated the hypothesis that impaired human neonatal CD4+ T cell immunity was due to reduced signaling by naive CD4+ T cells following engagement of the αβ-TCR/CD3 complex and CD28. Surprisingly, calcium flux following engagement of CD3 was significantly higher in neonatal naive CD4+ T cells from umbilical cord blood compared with naive CD4+ T cells from adult peripheral blood. [J Immunol] Abstract The Effects of Hedgehog on the RNA-Binding Protein Msi1 in the Proliferation and Apoptosis of Mesenchymal Stem Cells Investigators evaluated the direct effects of Hedgehog (Hh) signaling on the proliferation and apoptosis of human umbilical cord blood-derived mesenchymal stem cells as well as investigated potential downstream regulatory mechanisms that may be responsible for Hh signaling. [PLoS One] Full Article MicroRNAs miR-26a, miR-26b, and miR-29b Accelerate Osteogenic Differentiation of Unrestricted Somatic Stem Cells from Human Cord Blood Unrestricted somatic stem cells (USSC) are a rare population in human cord blood that can be induced into cells representative of all three germinal layers. Researchers analyzed the functional impact of miRNAs on the osteogenic differentiation in USSC. [BMC Genomics] Abstract | Full Article A Novel Reduced Intensity Conditioning Regimen Induces a High Incidence of Sustained Donor-Derived Neutrophil and Platelet Engraftment after Double-Unit Cord Blood Transplantation A preparative regimen of reduced intensity that can reliably engraft cord blood (CB) and be used as an alternative to either high-dose myeloablative or non-myeloablative conditioning is needed. Researchers evaluated double-unit CB transplantation in 30 patients with acute leukemia or myelodysplasia using a regimen of cyclophosphamide 50 mg/kg, fludarabine 150 mg/m2, thiotepa 10 mg/kg, and 400 cGy total body irradiation with cyclosporine-A/ mycophenolate mofetil immunosuppression. [Biol Blood Marrow Transplant] Abstract Double-Unit Cord Blood Transplantation after Myeloablative Conditioning for Patients with Hematological Malignancies: A Multicenter Phase II Study in Japan Scientists analyzed the outcome of 61 patients with hematological malignancies who were receiving double-unit cord blood transplantation after myeloablative conditioning performed as a prospective multi-center Phase II study. [Biol Blood Marrow Transplant] Abstract Impact of ABO-Mismatch on Risk of GvHD after Umbilical Cord Blood Transplantation Investigators analyzed the impact of ABO-mismatch on acute GvHD and chronic GvHD in recipients of single and double umbilical cord blood-hematopoietic cell transplants. [Bone Marrow Transplant] Abstract Mesenchymal Stromal Cell Supported Umbilical Cord Blood Ex Vivo Expansion Enhances Regulatory T Cells and Reduces Graft versus Host Disease Researchers explored the effect of ex vivo expansion of a single cord blood unit in a double cord blood transplantation setting on graft versus host disease and engraftment. [Cytotherapy] Abstract Pleiotrophin Is Involved in the Amniotic Epithelial Cell-Induced Differentiation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells into Dopaminergic Neuron-Like Cells Scientists investigated whether pleiotrophin is released by amniotic epithelial cells and whether it is involved in the differentiation of human umbilical cord blood-derived mesenchymal stem cells into dopaminergic neuron-like cells. [Neurosci Lett] Abstract An Improved Protocol for Ex Vivo Expansion of Highly Lytic NK Cells from Human Umbilical Cord Blood CD34+ Cells The authors describe an improved protocol based on multiple cytokines (SCF, Flt3-L, IL-15 and IGF-1) culture for obtaining a high number of lytic NK cells from human umbilical cord blood CD34+ cells. [Protoc Exchange] Full Article |