MePR: A Novel Human Mesenchymal Progenitor Model with Characteristics of Pluripotency Scientists report on the immortalization and characterization of novel Mesenchymal Progenitor (MePR) cell lines from amniotic fluid-derived human mesenchymal stem cells, whose biological properties are similar to primary amniocytes. [Stem Cells Dev] Abstract Baculovirus as an Ideal Radionuclide Reporter Gene Vector: A New Strategy for Monitoring the Fate of Human Stem Cells In Vivo The feasibility of utilizing recombinant baculoviruses carrying the sodium iodide symporter (NIS) reporter gene in monitoring stem cell therapy by radionuclide imaging was explored. Recombinant baculoviruses carrying NIS and green fluorescent protein reporter genes were constructed and used to infect human induced pluripotent stem cells, human embryonic stem cells and human umbilical cord blood mesenchymal stem cells. [PLoS One] Full Article Wnt Signaling Behaves as a “Master Regulator” in the Osteogenic and Adipogenic Commitment of Human Amniotic Fluid Mesenchymal Stem Cells Human amniotic fluid mesenchymal stem cells (huAFMSCs) are emerging as a promising therapeutic option in regenerative medicine. Investigators characterized huAFMSC phenotype and multipotentiality. [Stem Cell Rev] Full Article Reduced Frequency of High TLR4-Expressing CD14+ CD16+ Monocyte Subset in Cord Compared to Adult Blood Contributes to LPS Hyporesponsiveness in Newborns The human innate immune response to pathogens is not fully effective and mature until well into childhood, as exemplified by varying responses to TLR agonists in newborns compared to adults. To better understand the mechanistic basis for this age-related difference in innate immunity researchers compared TNFα production by monocytes from cord blood and adult blood in response to LAM (lipoarabinomannan from M. tuberculosis, TLR2 ligand) and LPS (lipopolysaccharide from E. coli, TLR4 ligand). [Clin Vaccine Immunol] Abstract Isolation of Functional Human Endothelial Cells from Small Volumes of Umbilical Cord Blood The authors showed that human endothelial cells can be isolated without the prior separation of blood components through the technique of diluted whole blood incubation utilizing commercially available human serum. [Ann Biomed Eng] Abstract Relative Biological Efficiency of Protons at Low and Therapeutic Doses in Induction of 53BP1/gH2AX Foci in Lymphocytes from Umbilical Cord Blood In order to evaluate DNA damage induced by protons at low and radiotherapeutic doses, lymphocytes from umbilical cord blood of the same four probands were irradiated in the dose range of 1 – 200 cGy with γ-rays and protons (200 MeV, irradiation in the Bragg peak). [Int J Radiat Biol] Abstract Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells in the Cultured Rabbit Intervertebral Disc: A Novel Cell Source for Disc Repair Researchers intended to confirm the potential for human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) to differentiate and display a chondrocyte-like phenotype after culturing in micromass and after injection into the rabbit intervertebral disc (IVD) explant culture. They also wanted to confirm hUCB-MSC survival after transplantation into the IVD explant culture. [Am J Phys Med Rehabil] Abstract Self-Renewing Human Bone Marrow Mesenspheres Promote Hematopoietic Stem Cell Expansion Researchers describe a method of isolating and culturing human bone marrow stromal stem/progenitor cells as nonadherent mesenchymal spheres. In culture conditions where they displayed a relatively undifferentiated phenotype, with decreased adherence to plastic and increased self-renewal, they promoted enhanced expansion of cord blood CD34+ cells through secreted soluble factors. Expanded hematopoietic stem cells were serially transplantable in immunodeficient mice and significantly increased long-term human hematopoietic engraftment. [Cell Rep] Abstract | Graphical Abstract |