Extensive Ex Vivo Expansion of Functional Human Erythroid Precursors Established from Umbilical Cord Blood Cells by Defined Factors Researchers report that ectopic expression of three genetic factors (Sox2, c-Myc and a shRNA against the TP53 gene) associated with iPSC derivation enables cord blood-derived erythroblasts to undergo extended expansion (~1068 fold in ~12 months) in a serum-free culture condition without change of cell identity or function. [Mol Ther] Abstract Human Amniotic Fluid Stem Cell Differentiation along Smooth Muscle Lineage Scientists showed for the first time that efficient smooth muscle cells can be obtained from human amniotic fluid stem cells (hAFSCs). Clonal lines were generated from c-kit+ hAFSCs. [FASEB J] Abstract The Activation of RhoC in Vascular Endothelial Cells Is Required for the S1P Receptor Type 2-Induced Inhibition of Angiogenesis Whereas the sphingosine-1-phosphate receptor type 1 (S1P1R) regulates endothelial barrier function by coupling to Gαi and the monomeric GTPase Rac1, the signaling pathways involved in the S1P-induced regulation of angiogenesis are ill defined. Scientists therefore studied the sprouting of human umbilical vein endothelial cells in vitro and analyzed the activation of the RhoGTPases RhoA and RhoC. [Cell Signal] Abstract Assessment of Umbilical Cord Tissue as a Source of Mesenchymal Stem Cell/Endothelial Cell Mixtures for Bone Regeneration Compared with bone marrow mesenchymal stem cells (MSCs), umbilical cord MSCs displayed significantly lower (range 4-240-fold) basal levels of bone-related transcripts, but their phenotypes were similar (CD73+, CD105+, CD90+, CD45– and CD31–). [Regen Med] Abstract Mesenchymal Stem Cells from Human Umbilical Cord Express Preferentially Secreted Factors Related to Neuroprotection, Neurogenesis, and Angiogenesis Mesenchymal stem cells (MSCs) found in the Wharton’s jelly of the human umbilical cord are easily obtained and are capable of transplantation without rejection. Investigators isolated MSCs from Wharton’s jelly and bone marrow and compared their secretomes. [PLoS One] Full Article Lipopolysaccharides Shapes the Human Wharton’s Jelly-Derived Mesenchymal Stem Cells In Vitro The expression and role of toll-like receptor 4 in human Wharton’s jelly-derived mesenchymal stem cells (hWJ-MSCs) was investigated using a model of lipopolysaccharide (LPS). Proliferation, apoptosis, and the expression of paracrine factors in hWJ-MSCs primed with LPS were analyzed. [Cell Physiol Biochem] Full Article Dominant Negative MCP-1 Blocks Human Osteoclast Differentiation: Gene Expression in Osteoclasts Derived from Cord Blood CFU-GM Myeloid Precursors Human osteoclasts were differentiated using receptor activator of NFκB ligand and macrophage colony stimulating factor from colony forming unit-granulocyte macrophage (CFU-GM) precursors of the myeloid lineage grown from umbilical cord blood. [J Cell Biochem] Abstract |