CD34+VEGFR-3+ Progenitor Cells Have a Potential to Differentiate towards Lymphatic Endothelial Cells The authors assessed differentiation of a novel population of endothelial progenitor cells (EPCs) towards lymphatic endothelial cells and their lymphatic formation. CD34+VEGFR-3+ EPCs were isolated from mononuclear cells of human cord blood by fluorescence-activated cell sorting. [J Cell Mol Med] Full Article Sustained Donor Engraftment in Recipients of Double-Unit Cord Blood Transplantation Is Possible Despite Donor-Specific HLA-Antibodies Investigators evaluated the influence of pre-existing human leukocyte antigen (HLA)-antibodies on engraftment in 82 double-unit cord blood recipients (median age 48 years) transplanted for hematologic malignancies. [Biol Blood Marrow Transplant] Abstract Gene and miRNA Expression Profiles of Hematopoietic Progenitor Cells Vary Depending on Their Origin Hematopoietic progenitors cells from G-CSF mobilized peripheral blood, bone marrow, or umbilical cord blood have different biological properties and different kinetic of engraftment post-transplantation, which might be at least in part explained by a different gene and miRNA expression pattern. In order to assess differences in gene and miRNA expression researchers analyzed whole genome expression profile as well as the expression of 384 miRNAs in CD34+ cells isolated from 18 healthy individuals (6 individuals per subtype of hematopoietic progenitor cells source). [Biol Blood Marrow Transplant] Abstract Monocytes Are Essential for the Neuroprotective Effect of Human Cord Blood Cells following Middle Cerebral Artery Occlusion in Rat Systemic administration of human umbilical cord blood (HUCB) mononuclear cells following middle cerebral artery occlusion in the rat reduces infarct size and, more importantly, restores motor function. The HUCB cell preparation is composed of immature T-cells, B-cells, monocytes and stem cells. Scientists examined whether the beneficial effects of HUCB injection were attributable to one of these cell types. [Mol Cell Neurosci] Abstract Lipopolysaccharide Impaired the Functional Activity of Endothelial Colony-Forming Cells Researchers investigated possible effects of lipopolysaccharide on the functional activity of endothelial colony-forming cells (ECFCs). ECFCs were isolated from human umbilical cord blood and characterized. [Respiration] Abstract Induction of Multipotency in Umbilical Cord-Derived Mesenchymal Stem Cells Cultivated under Suspension Conditions Scientists isolated mesenchymal stem cells from umbilical cord tissue at the single-cell level, by treatment with trypsin, followed by cultivation under suspension conditions to form a colony. [Cell Stress Chaperones] Abstract Cord Blood Graft Composition Impacts the Clinical Outcome of Allogeneic Stem Cell Transplantation Investigators found that above-median frequencies of CD69+ T cells, naïve CD8+ T cells, and CD127+ B cells in the cord blood graft were each associated with significantly improved patient survival. [Transpl Infect Dis] Abstract Prostaglandin E2 Promotes Survival of Naive UCB T Cells via the Wnt/β-Catenin Pathway and Alters Immune Reconstitution after UCBT Exposure of umbilical cord blood (UCB) to dimethyl-prostaglandin E2 (dmPGE2) increases hematopoietic stem cells in vivo. The authors determined that exposure of UCB T lymphocytes to dmPGE2 modified Wnt signaling resulting in T cell factor-mediated transcription. [Blood Cancer J] Full Article |