The Unfolded Protein Response Governs Integrity of the Hematopoietic Stem-Cell Pool during Stress Investigators showed that human hematopoietic stem cells are predisposed to apoptosis through strong activation of the PERK branch of the unfolded protein response after endoplasmic reticulum stress, whereas closely related progenitors exhibit an adaptive response leading to their survival. [Nature] Abstract OKT3 Antibody Pre-Treatment Prevents Xenogeneic GvHD and Allows for Reliable Initiation of Xenografts from Unfractionated Human Hematopoietic Tissues OKT3 treatment of unfractionated cord blood resulted in robust engraftment of primary and secondary mice that was indistinguishable from grafts obtained using purified CD34+ cells. [Blood] Abstract CD52 Is a Molecular Target in Advanced Systemic Mastocytosis To study the mechanisms of CD52 expression and the value of this antigen as a potential therapeutic target, investigators generated a human mast cell (MC) cell line, designated MCPV-1, by lentiviral immortalization of cord blood-derived MC progenitor cells. [FASEB J] Abstract Human Lymphoid Development in the Absence of Common γ-Chain Receptor Signaling Researchers applied recent data that have revised our understanding of the earliest stages of lymphoid commitment in human bone marrow (BM) to determine the requirement for signaling through IL-2RG and JAK3 in normal development of human lymphoid progenitors. BM samples from SCID patients with IL-2RG or JAK3 deficiency, which produce similar “T-NK-B+” clinical phenotypes, were compared with normal BM and umbilical cord blood as well as BM from children on enzyme treatment for adenosine deaminase-deficient SCID. [J Immunol] Abstract CD4+ T Cells from Human Neonates and Infants Are Poised Spontaneously to Run a Nonclassical IL-4 Program The authors showed the appearance of a distinct CD4+ T cell subset expressing IL-4 at an early stage of development in infant adenoids and cord blood that is lost during aging. [J Immunol] Abstract Negative Selection by Apoptosis Enriches Progenitors in Naïve and Expanded Human Umbilical Cord Blood Grafts The influence of TNF-α and Fas-ligand on viability and function was evaluated in fresh- and expanded-umbilical cord blood cells. CD34+ progenitors and T cells display outstanding survival, whereas ~30% and >50% B lymphocytes and myeloid cells undergo spontaneous apoptosis within 24 and 48 hours, respectively. [Bone Marrow Transplant] Abstract A Prenatal Prediction Model for Total Nucleated Cell Count Increases the Efficacy of Umbilical Cord Blood Banking Scientists investigated prenatal clinical predictors for total nucleated cell count that would help to identify successful umbilical cord blood donors already on admission to the delivery unit. [Transfusion] Abstract Evaluation of Intracellular and Extracellular Trehalose as a Cryoprotectant of Stem Cells Obtained from Umbilical Cord Blood Results showed the effectiveness of the solution containing intracellular and extracellular trehalose in the cryopreservation of cord blood cells, demonstrating that trehalose may be an optimal cryoprotectant when present both inside and outside of cells. [Cryobiology] Abstract |