Genome-Wide DNA Methylation Analysis in Precursor B-Cells In order to elucidate the influence of DNA methylation during B-cell development, genome-wide DNA methylation status of pro-B, pre-BI, pre-BII, and naive-B-cells isolated from human umbilical cord blood was determined using the methylated CpG island recovery assay followed by next generation sequencing. [Epigenetics] Abstract Epigenome-Wide Analysis of Neonatal CD4 T-Cell DNA Methylation Sites Potentially Affected by Maternal Fish Oil Supplementation To determine whether the biological effects of maternal omega-3 polyunsaturated fatty acids supplementation are mediated through DNA methylation, researchers analyzed CD4+ T-cells purified from cryo-banked cord blood samples from a previously conducted clinical trial. [Epigenetics] Abstract Human Mesenchymal Stem Cells Possess Different Biological Characteristics but Do Not Change Their Therapeutic Potential when Cultured in Serum Free Medium Growth, multipotency, surface antigen expression, telomerase, immunosuppressive ability, gene expression profile and genomic stability of human umbilical cord mesenchymal stem cells cultured in serum free medium and serum-containing medium were analyzed and compared side by side. [Stem Cell Res Ther] Abstract | Full Article Pre-Treatment of Human Umbilical Cord-Derived Mesenchymal Stem Cells with Interleukin-6 Abolishes Their Growth-Promoting Effect on Gastric Cancer Cells The authors focused on the phenotype and function of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) pre-treated with interleukin-6 (IL-6) in gastric cancer. They found that the protein levels of α-smooth muscle actin were upregulated, and phosphorylated nuclear factor-κB protein levels were downregulated in the hUCMSCs pre-treated with IL-6, as shown by western blot analysis. [Int J Mol Med] Abstract | Download Full Article Bone Marrow Mesenchymal Stem Cells Suppressing Activation of Allogeneic Cytokine-Induced Killer/Natural Killer Cells Either by Direct or Indirect Interaction Investigators explored the effect of mesenchymal stem cells on the cell cycle, apoptosis rate and the expression of CD69, an activation marker, on the allogeneic cord blood derived cytokine-induced killer/natural killer cells. [Cell Biol Int] Abstract Growth Factors Mediated Differentiation of Mesenchymal Stem Cells to Cardiac Polymicrotissue Using Hanging Drop and Bioreactor Investigators created cardiac polymicrotissue from human umbilical cord matrix mesenchymal stem cells using a combination of growth factors (sphingosine-1-phosphate and suramin) and techniques (hanging drop and bioreactor). [Cell Biol Int] Abstract Phenotypical and Functional Characteristics of Mesenchymal Stem Cells Derived from Equine Umbilical Cord Blood Investigators optimized methodology for isolation, cultural characterization, differentiation and immunophenotyping of mesenchymal stem cells from equine umbilical cord blood. [Cytotechnology] Abstract |