DERMAL STEM CELLS AND TISSUE REGENERATION Confluence Induced Squamous Differentiation is not Accompanied by Changes in H3K27me3 Repressive Epigenetic Mark Scientists examined the binding of trimethylated H3K27 to the promoters of proliferation or differentiation genes in keratinocytes undergoing squamous differentiation in vitro and in vivo. [J Invest Dermatol] Abstract Translational Repression Protects Human Keratinocytes from UVB-Induced Apoptosis through a Discordant eIF2 Kinase Stress Response Researchers determined that ultraviolet B (UVB) irradiation is a potent inducer of eukaryotic initiation factor 2 (eIF2~P) in keratinocytes, leading to decreased levels of translation initiation. [J Invest Dermatol] Abstract Arachidonic Acid Promotes Skin Wound Healing through Induction of Human MSC Migration by MT3-MMP-Mediated Fibronectin Degradation The authors investigated the skin wound-healing effect of arachidonic acid (AA)-stimulated human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) in vivo and its molecular mechanism in vitro. They found that transplantation of hUCB-MSCs pre-treated with AA enhanced wound filling, re-epithelization, and angiogenesis in a mouse skin excisional wound model. [Cell Death Dis] Full Article A Two-Stepped Culture Method for Efficient Production of Trichogenic Keratinocytes Researchers found that the short-term co-culture of hair follicle outer root sheath keratinocytes with dermal papilla cells both enhanced Wnt/β-catenin signaling and upregulated the expression of hair follicle specific genes, including K6, K16, K17 and K75, in keratinocytes. [Tissue Eng Part C Methods] Abstract Paracrine Action of Mesenchymal Stromal Cells Delivered by Microspheres Contributes to Cutaneous Wound Healing and Prevents Scar Formation in Mice Microspheres successfully delivered mesenchymal stromal cells into wound margins and significantly accelerated wound healing and concomitantly reduced the fibrotic activities of cells within the wounds and excessive accumulation of extracellular matrix as well as the transforming growth factor-β1/transforming growth factor-β3 ratio. [Cytotherapy] Abstract SKIN CANCERS AND DISORDERS Transglutaminase Is Required for Epidermal Squamous Cell Carcinoma Stem Cell Survival Transglutaminase type II (TG2) was determined to be highly elevated in epidermal cancer stem cells (ECS cells) and TG2 knockdown or suppression of TG2 function with inhibitors reduced ECS cell survival, spheroid formation, matrigel invasion and migration. [Mol Cancer Ther] Abstract RBM28, a Protein Deficient in ANE Syndrome, Regulates Hair Follicle Growth Via miR-203 and p63 When RBM28 was down-regulated in human hair follicle (HF) organ cultures, investigators observed catagen induction and HF growth arrest, indicating that RBM28 is necessary for normal hair growth. [Exp Dermatol] Abstract Basal Level of Autophagy Is Increased in Aging Human Skin Fibroblasts In Vitro, but not in Old Skin The authors compared the basal levels of intracellular autophagy in serially passaged human facial skin fibroblasts undergoing aging and replicative senescence in vitro, and ex vivo in the skin biopsies from the photo-protected and photo-exposed area of the arms of 20 healthy persons of young and old ages. [PLoS One] Full Article A Role of Myocardin Related Transcription Factor-A (MRTF-A) in Scleroderma Related Fibrosis Researchers demonstrated that nuclear translocation of MRTF-A in scleroderma tissues occurs in keratinocytes, endothelial cells, infiltrating inflammatory cells, and dermal fibroblasts, consistent with enhanced signaling in multiple cell lineages exposed to the stiff extracellular matrix. [PLoS One] Abstract Association of SIRT1 and Tumor Suppressor Gene TAp63 Expression in Head and Neck Squamous Cell Carcinoma Scientists investigated whether SIRT1 promotes differentiation in head and neck squamous cell carcinoma (HNSCC) cells by studying the correlations between the expression of SIRT1 and several genes implicated in stemness or differentiation in HNSCC-derived cell lines. [Tumour Biol] Abstract |