DERMAL STEM CELLS & TISSUE REGENERATION The Androgen Receptor Antagonizes Wnt/β-Catenin Signaling in Epidermal Stem Cells The authors demonstrated that during epidermal development and homeostasis there is reciprocal activation of the androgen receptor and β-catenin in cells of the hair follicle bulb. [J Invest Dermatol] Abstract | Full Article Establishing Correlations in the En-Mass Migration of Dermal Fibroblasts on Oriented Fibrillar Scaffolds Investigators developed a technique for preparation of three dimensional fibrillar scaffolds where the fiber diameters and the angles between adjacent fiber layers could be precisely controlled. Using the agarose droplet method, they were able to make accurate determinations of the dependence of the migration speed, focal adhesion distribution, and nuclear deformation on the fiber diameter, fiber spacing, and angle between adjacent fiber layers. [Acta Biomater] Abstract Amniotic Fluid Stem Cells Provide Considerable Advantages in Epidermal Regeneration: B7H4 Creates a Moderate Inflammation Microenvironment to Promote Wound Repair Researchers examined the advantages of using human amniotic fluid stem (hAFS) cells in skin wound healing. In vitro, hAFS cells differentiate into keratinocytes. During wound repair, hAFS cells did not directly secrete repair-related factors, such as bFGF, VEGF, CXCL12, TGF-β1 and KGF, and provided a moderate inflammation reaction with lower expression of IL-1β, IL-6, TNF-α, Cox2 and Mac3. [Sci Rep] Full Article SKIN CANCERS & DISORDERS Sox9 Controls Self-Renewal of Oncogene Targeted Cells and Links Tumor Initiation and Invasion Using a genetic mouse model of basal cell carcinoma, scientists showed that Sox9 is expressed from the earliest step of tumor formation in a Wnt/β-catenin-dependent manner. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Aquaporin-3-Mediated Hydrogen Peroxide Transport Is Required for NF-κB Signaling in Keratinocytes and Development of Psoriasis Researchers showed that hydrogen peroxide, imported via aquaporin 3 (AQP3), was involved in nuclear factor-κB (NF-κB) signaling in keratinocytes and in the pathogenesis of psoriasis. [Nat Commun] Abstract Targeting Melanoma with NT157 by Blocking Stat3 and IGF1R Signaling The authors showed that NT157 targets not only IGF1R-IRS1/2, as previously reported, but also the Stat3 signaling pathway and demonstrated remarkable anti-cancer characteristics in A375 human melanoma cells and in a metastatic melanoma model in mice. [Oncogene] Abstract CCN1, a Pro-Inflammatory Factor, Aggravates Psoriasis Skin Lesions by Promoting Keratinocyte Activation Scientists demonstrated that CCN1 is highly expressed in the lesional skin of psoriasis patients, as well as that of imiquimod- and IL-23-treated psoriasis-like mice. They showed that blocking CCN1 function in vivo attenuated epidermal hyperplasia and inflammation in psoriasis-like mice. [J Invest Dermatol] Abstract Ingenol Mebutate Signals via PKC/MEK/ERK in Keratinocytes and Induces Interleukin Decoy Receptors IL1R2 and IL13RA2 Researchers elucidated direct effects of ingenol mebutate (IM) on primary keratinocytes, patient-derived squamous cell carcinoma (SCC) cells and a SCC cell line. Transcriptional profiling followed by pathway analysis was performed on IM-treated primary keratinocytes and patient-derived SCC cells to find key mediators and identify the mechanism of action. [Mol Cancer Ther] Abstract In Vivo Fluorescence Immunohistochemistry: Localization of Fluorescently Labeled Cetuximab in Squamous Cell Carcinomas In this study of a systemically administered near-infrared fluorescently labeled therapeutic antibody, cetuximab-IRDye800CW, scientists showed that by optical molecular imaging they were able to evaluate localization of fluorescently labeled cetuximab. [Sci Rep] Full Article |