DERMAL STEM CELLS & TISSUE REGENERATION Epidermal β-Catenin Activation Remodels the Dermis via Paracrine Signaling to Distinct Fibroblast Lineages Sustained epidermal Wnt/β-catenin signaling expands the stem cell compartment and induces ectopic hair follicles. This is accompanied by extensive fibroblast proliferation and extracellular matrix remodeling in the underlying dermis. Scientists showed that epidermal hedgehog and transforming growth factor-beta signaling mediate the dermal changes. [Nat Commun] Full Article Hierarchy of Cellular Decisions in Collective Behavior: Implications for Wound Healing To determine which individual cell behaviors represent the most promising targets to engineer re-epithelialization, investigators examined collective and individual responses of HaCaT keratinocytes seeded upon polyacrylamide gels of three stiffnesses and treated with a range of epidermal growth factor doses. [Sci Rep] Full Article Comparative Transcriptomic Profiling of Hydrogen Peroxide Signaling Networks in Zebrafish and Human Keratinocytes: Implications toward Conservation, Migration and Wound Healing Comparative transcriptome analysis using RNA-seq data from larval zebrafish and previously reported microarray data from a human epidermal keratinocyte line showed that H2O2 activates conserved cell migration, adhesion, cytoprotective and anti-apoptotic programs in both zebrafish and human keratinocytes. [Sci Rep] Full Article Nitric Oxide Chemical Donor Affects the Early Phases of In Vitro Wound Healing Process An artificial wound in a confluent monolayer of human keratinocyte HaCaT cells or mouse embryo fibroblast Swiss NIH 3T3 cells was used to analyze the effects of the nitric oxide chemical donor, S-nitroso-N-acetylpenicillamine. [J Cell Physiol] Abstract SKIN CANCERS & DISORDERS Self-Targeting of TNF-Releasing Cancer Cells in Preclinical Models of Primary and Metastatic Tumors The authors genetically engineered mouse mammary adenocarcinoma, melanoma, and Lewis lung carcinoma cells to produce and release murine tumor necrosis factor alpha (TNF). They showed that these malignant cells home to tumors, locally release TNF, damage neovascular endothelium, and induce massive cancer cell apoptosis. [Proc Natl Acad Sci USA] Abstract | Full Article Primary and Metastatic Cutaneous Melanomas Express ALK through Alternative Transcriptional Initiation Researchers determined whether anaplastic lymphoma kinase (ALK) was expressed in nonspitzoid primary and metastatic cutaneous melanomas. [Am J Surg Pathol] Abstract Phosphorylation of eIF4E Serine 209 Is Associated with Tumor Progression and Reduced Survival in Malignant Melanoma Researchers determined the association of eukaryotic translation initiation factor 4E (eIF4E) and phospho-eIF4E expression in melanocytic lesions with malignant conversion, metastatic potential and patient survival. [Br J Cancer] Abstract Pluripotency Markers Are Differentially Induced by MEK Inhibition in Thyroid and Melanoma BRAFV600E Cell Lines Cell line models of melanoma and thyroid carcinoma were treated with the MEK inhibitor PD0325901. Treated and naive samples were assayed for expression of key members of the MAPK pathway. [Cancer Biol Ther] Abstract |