Dermal Cell News Volume 4.10 | Mar 26 2018

TOP STORY

Pivotal Role of NAMPT in the Switch of Melanoma Cells toward an Invasive and Drug-Resistant Phenotype

Nicotinamide phosphoribosyltransferase (NAMPT) inhibition decreased melanoma cell proliferation both in vitro and in vivo, while forced NAMPT expression rendered melanoma cells resistant to PLX4032. NAMPT expression induced transcriptomic and epigenetic reshufflings that steered melanoma cells toward an invasive phenotype associated with resistance to targeted therapies and immunotherapies. [Genes Dev] Abstract

(Ranked by impact factor of the journal)

DERMAL STEM CELLS & TISSUE REGENERATION

Delayed Allogeneic Skin Graft Rejection in CD26-Deficient Mice

CD26 gene-knockout mice were used to investigate the potential role of CD26/dipeptidyl peptidase-4 (DPPIV) in allogeneic skin graft rejection by tail-skin transplantation. The concentration of IL-17 in serum and the percentage of cells secreting IL-17 in mouse peripheral blood lymphocytes (MPBLs) were both significantly lower, while the percentage of regulatory T cells was significantly higher in MPBLs of CD26^–/– mice than in those of CD26^+/+ mice. [Cell Mol Immunol] Abstract

Organic Osmolytes Preserve the Function of the Developing Tight Junction in Ultraviolet B-Irradiated Rat Epidermal Keratinocytes

Since tight junctions (TJs) are intimately associated with control of water homeostasis in skin, researchers hypothesised that there may be a direct influence of osmolytes on TJ development. Exposure of rat epidermal keratinocytes to a single dose of ultraviolet B radiation reduced the function of developing TJs. [Sci Rep] Full Article

Epigallocatechin-3-Gallate Partially Restored Redox Homeostasis in Arsenite-Stressed Keratinocytes

Scientists focused on the effect of epigallocatechin‐3‐gallate (EGCG) on redox status of arsenite (AsIII)‐stressed normal human keratinocytes, HaCaT cells. EGCG was effective not only in reducing AsIII‐induced nuclear expression of Nrf2 and Nrf2Ser40 but also in increasing nuclear expression of Keap1 both at protein and mRNA level. [J Appl Toxicol] Abstract

Punicalagin and (-)-Epigallocatechin-3-Gallate Rescue Cell Viability and Attenuate Inflammatory Responses of Human Epidermal Keratinocytes Exposed to Airborne Particulate Matter PM10

Investigators evaluated whether polyphenolic antioxidants attenuate the inflammatory responses of airborne particulate matter with a diameter of <10 μm (PM10)-exposed keratinocytes. Assays were performed to determine cell viability, production of reactive oxygen species, and expression of NADPH oxidases, proinflammatory cytokines, and matrix metalloproteinase-1. [Skin Pharmacol Physiol] Abstract

PER, a Circadian Clock Component, Mediates the Suppression of MMP-1 Expression in HaCaT Keratinocytes by cAMP

The authors found PERIOD protein, a morning clock component, represses the expression of matrix metalloproteinase-1 (MMP-1) in human keratinocytes by using a PER-knockdown strategy. They revealed PER3 suppresses the expression of MMP-1 via cAMP signaling pathway. [Molecules] Full Article

SKIN CANCERS & DISORDERS

Induction of Telomere Dysfunction Prolongs Disease Control of Therapy-Resistant Melanoma

Scientists demonstrated the superior efficacy of 6-thio-2′-deoxyguanosine both in vitro and in vivo that results in telomere dysfunction, leading to apoptosis and cell death in various pre-clinical models of therapy-resistant melanoma cells. [Clin Cancer Res] Abstract | Press Release

Inflammatory Cytokine-Mediated Induction of Serine Racemase in Atopic Dermatitis

Researchers demonstrated that serine racemase (SR) was expressed in the epidermis of wild-type (WT) mice but not in SR knockout mice. In addition, SR immune-reactivity was only found in the granular and cornified layers of the epidermis in WT mice. [J Cell Mol Med] Abstract | Full Article

Regulation of IL-22BP in Psoriasis

Investigators used the TLR7 agonist Imiquimod (IMQ) to induce a psoriasis-like skin disease in mice and found a strong downregulation of IL-22 binding protein in the affected skin as well as in the lymph nodes of animals treated with IMQ. [Sci Rep] Full Article

Androgen Modulation of Wnt/β-Catenin Signaling in Androgenetic Alopecia

The authors describe recent studies on the role of androgens and androgen receptor transactivation activity in modulating the Wnt/β-catenin signaling in the dermal papilla cells of the balding scalp in androgenetic alopecia. [Arch Dermatol Res] Abstract

Visit our reviews page to see a complete list of reviews in the dermal cell research field.

Biomedical Group Brings California’s Regenerative Medicine Research to the National Stage

Biocom’s Regenerative Medicine Fly-In brought ten participants from seven companies, including researchers, regulatory experts and business executives to Washington to meet with officials at the National Institutes of Health and the FDA, as well as U.S. Representatives Susan Davis, Scott Peters, Lou Correa and Judy Chu. Participants had the unique opportunity to discuss new advances in regenerative medicine and the successes and challenges they face. [Biocom (Business Wire, Inc.)] Press Release

Science Magazine Recognizes Frequency Therapeutics’ PCA Regeneration Technology as a Breakthrough in Regenerative Medicine

Frequency Therapeutics announced that Will McLean, Ph.D., Co-founder and Vice President, Biology and Regenerative Medicine, has received a finalist award for the inaugural Sartorius & Science Prize for Regenerative Medicine & Cell Therapy. [Frequency Therapeutics] Press Release

Duke’s Mishandling of Misconduct Prompts New U.S. Government Grant Oversight

The U.S. National Institutes of Health imposed unusual new requirements on researchers based at Duke University in Durham, North Carolina, who receive federal funds. The changes are a response to concerns over how the institution handled recent cases involving research misconduct and grant management. [ScienceInsider] Editorial

Massive Cyberhack by Iran Allegedly Stole Research from 320 Universities, Governments, and Companies

Nine Iranians working on behalf of the Islamic Revolutionary Guard Corps hacked the computers of 7998 professors at 320 universities around the world over the past five years, an indictment filed by a federal grand jury alleges. [ScienceInsider] Editorial

Final 2018 Budget Deal Should Help the National Science Foundation in 2019, Too

The 4% increase for the National Science Foundation (NSF) in Alexandria, Virginia, in the 2018 omnibus spending bill hammered out by congressional leaders this week may be modest next to what its peer science agencies received. But it does offer NSF officials more breathing room to fund some major initiatives starting next year. [ScienceInsider] Editorial

Final 2018 Budget Bill Eases Biomedical Researchers’ Policy Worries

The 2018 omnibus spending bill released is cheering biomedical researchers. Not only because of the 8.8% raise it gives the National Institutes of Health—its largest in 15 years—but also because it blocks or drops several proposed policy changes that had concerned the community. [ScienceInsider] Editorial

NEW 3D Cell Culture 2018
June 5-7, 2018
Freiburg, Germany

Visit our events page to see a complete list of events in the community.

NEW Faculty Position – Developmental Biology and Regenerative Medicine (Seattle Children’s Research Institute)

Academic Dermatopathologist – Non-Melanoma Skin Cancer (Harvard Medical School)

Postdoctoral Research Associate – Melanoma (University of North Texas Health Science Center)

Postdoctoral Position – Epidermal Development and Differentiation (National Institutes of Health)

Postdoctoral Associate – Biomedical Engineering (University of Minnesota)

Postdoctoral Fellow – Cancer Research (Indiana University School of Medicine)

Scientific Editor – Cancer Cell (Elsevier)

Postdoctoral Fellow – Molecular Biology (Cold Spring Harbor Laboratory)

PhD Position – Melanoma (University of Luxembourg)

PhD or Postdoctoral Position – Tissue Repair, Stem Cell Fate and Tumor Heterogeneity (Université Libre de Bruxelles)

Faculty Positions – Cancer and Stem Cell Biology (Sun Yat-Sen University)

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