Dermal Cell News Volume 5.03 | Jan 28 2019

TOP STORY

Improved TMC1 Gene Therapy Restores Hearing and Balance in Mice with Genetic Inner Ear Disorders

Researchers reported restoration of function in inner and outer hair cells, enhanced hair cell survival, restoration of cochlear and vestibular function, restoration of neural responses in auditory cortex and recovery of behavioral responses to auditory and vestibular stimulation. [Nat Commun] Full Article

(Ranked by impact factor of the journal)

DERMAL STEM CELLS & TISSUE REGENERATION

scRNA-Seq Reveals Distinct Stem Cell Populations That Drive Hair Cell Regeneration after Loss of Fgf and Notch Signaling

Ordering of support cells along a developmental trajectory identified self-renewing cells and genes required for hair cell differentiation. scRNA-Seq analyses of fgf3 mutants, in which hair cell regeneration was increased, demonstrated that Fgf and Notch signaling inhibited proliferation of support cells in parallel by inhibiting Wnt signaling. [Elife] Full Article

Study of 3D-Printed Chitosan Scaffold Features after Different Post-Printing Gelation Processes

Three different gelation media were selected and the 3D chitosan scaffolds were tested in terms of biocompatibility toward fibroblast as skin associated human cell line. [Sci Rep] Full Article

A Novel Target for the Promotion of Dermal Wound Healing: Ryanodine Receptors

The effect of ryanodine receptors on wound healing, microcirculation and inflammation was assessed in an in vivo mouse wound healing model, using skin fold chambers in the dorsal region, and in a HaCaT cell scratch wound assay in vitro. [Toxicol Appl Pharmacol] Abstract

SKIN CANCERS & DISORDERS

MALAT1-KTN1-EGFR Regulatory Axis Promotes the Development of Cutaneous Squamous Cell Carcinoma

Functional studies using in vitro cellular and in vivo xenograft models confirmed the pro-carcinogenic roles of MALAT1 in cutaneous squamous cell carcinom. Further, MALAT1 was identified to regulate epidermal growth factor receptor (EGFR) protein expression but did not affect EGFR mRNA expression. [Cell Death Differ] Abstract

Thymine DNA Glycosylase as a Novel Target for Melanoma

Scientists showed that thymine DNA glycosylase knockdown in melanoma cell lines caused cell cycle arrest, senescence, and death by mitotic alterations; altered the transcriptome and methylome; and impaired xenograft tumor formation. [Oncogene] Full Article

Tspan8-β-Catenin Positive Feedback Loop Promotes Melanoma Invasion

Investigators demonstrated that β-catenin stabilization was a molecular signal subsequent to the onset of Tspan8 expression, and that, in turn, β-catenin triggered the direct transcriptional activation of Tspan8 expression, leading to melanoma invasion. [Oncogene] Abstract

A Polymorphic Variant in p19^Arf Confers Resistance to Chemically-Induced Skin Tumors by Activating the p53 Pathway

Researchers reported the cyclin-dependent kinase inhibitor gene Cdkn2a/p19^Arf as a major responsible gene for the skin tumor modifier of MSM 3 (Stmm3) locus. They provided evidence that the function of Stmm3 was dependent on p53 and that p19^ArfMSM confers stronger resistance to papillomas than p16^Ink4aMSM in vivo. [J Invest Dermatol] Abstract

IL-17 and IL-22 Promote Keratinocyte Stemness in the Germinative Compartment in Psoriasis

Treatment with IL-17 and IL-22 induced upregulation of p63, CD44 and CD29 in normal keratinocytes (KCs) and increased the colony-forming efficiency and long-term proliferative capacity, reflecting increased stem cell-like characteristics in the KC population. [J Invest Dermatol] Abstract

Quantitative Analysis of Melanin Content in a Three-Dimensional Melanoma Cell Culture

The authors compared melanin synthesis in B16 murine melanoma cells grown in two-dimensional and three-dimensional culture environments. B16 cells instantly formed an aggregate in a hanging-drop culture, and synthesized melanin efficiently without treatment of alpha-melanocyte stimulating hormone. [Sci Rep] Full Article

Hinokitiol Suppresses Growth of B16 Melanoma by Activating ERK/MKP3/Proteosome Pathway to Downregulate Survivin Expression

Exposure of melanoma B16-F10 cells to hinokitiol significantly inhibited colony formation and cell viability in a time and concentration-dependent manner. The hinokitiol-treated cells exhibited apoptotic features in a morphological assay. [Toxicol Appl Pharmacol] Abstract | Graphical Abstract

A Human Skin Model Recapitulates Systemic Sclerosis Dermal Fibrosis and Identifies COL22A1 as a TGFβ Early Response Gene that Mediates Fibroblast to Myofibroblast Transition

The authors characterized the regulation and functional impact of a previously unreported gene in the setting of dermal fibrosis, COL22A1, and showed that silencing COL22A1 significantly reduced TGFβ-induced ACTA2 expression. COL22A1 expression was significantly increased in dermal fibroblasts from patients with systemic sclerosis. [Genes (Basel)] Abstract | Full Article

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Sunbeds and Melanoma Risk: Time to Close the Debate

Investigators discuss the real impact of sunbed use on melanoma risk in light of the most recently published evidence. [Curr Opin Oncol] Abstract

Visit our reviews page to see a complete list of reviews in the dermal cell research field.

Dr. Alice Gottlieb to Present Hidradenitis Suppurativa Findings

XBiotech announced that Dr. Alice Gottlieb, MD, PhD, will be giving the presentation “Bermekimab Shows Efficacy for Treating Hidradenitis Suppurativa, Including Marked Reduction in Pain.” [Press release from XBiotech Inc. discussing research to be presented at American Academy of Dermatology (AAD) Annual Meeting, Washington, D.C.] Press Release

Novartis Data Show Psoriasis Patients Treated with Cosentyx^® Reported Improvements in Quality of Life Measures as Early as Week 4

Novartis announced results from a pooled analysis of four Phase III clinical trials demonstrating patients with moderate-to-severe plaque psoriasis treated with Cosentyx^® reported improvements in mobility, self-care, and usual activities components of the EQ-5D-3L questionnaire as early as week four when compared to placebo in patients who reported problems at baseline. [Press release from Novartis AG (PR Newswire Association LLC) discussing research presented at 15th Annual Maui Derm for Dermatologists Conference, Wailea] Press Release

STEMCELL Technologies Launches mTeSR™ Plus, a Next-Generation Culture System for Human ES and iPS Cell Maintenance

STEMCELL Technologies has released mTeSR™ Plus, an enhanced version of mTeSR™1, the most widely published feeder-free human pluripotent stem cell maintenance medium. The new medium has undergone successful beta testing with several members of the Stem Cell COREdinates group and has completed rigorous in-house validation testing. [STEMCELL Technologies Inc.] Press Release

Horizon Discovery and Rutgers University Form Exclusive Partnership to Develop Next Generation Novel Gene Editing Technology

Horizon Discovery Group plc announced that it has entered into an exclusive strategic partnership with Rutgers University to develop and commercialise a novel gene editing technology, known as base editing. [Horizon Discovery Group plc (Business Wire, Inc.)] Press Release

US Science Agencies Reopen after Shutdown

On January 25, lawmakers ended the longest partial government shutdown in US history and agreed to open federal agencies for three weeks as they negotiate President Donald Trump’s demand for a wall along the southern border. Scientists at the National Science Foundation, Fish & Wildlife Service, NASA, and other research offices that had been closed for more than a month will now return to work – but the month-long shutdown will have a lasting effect. [The Scientist] Editorial

Kosovo Academics Brace for Controversial Ethnic Land Swaps

A proposed land swap between Serbia and Kosovo, which is designed to help end a decades-long ethnic conflict in the region, could threaten rare attempts at multi-ethnic research collaboration and education. The exchange aims to settle ownership of the remaining disputed areas between the two hostile neighbors, but it could also result in academic institutions finding themselves in a different, essentially enemy, country. [Nature News] Editorial

NEW Gordon Research Conference: Tissue Repair & Regeneration
June 9-14, 2019
New London, New Hampshire, United States

Visit our events page to see a complete list of events in the community.

NEW PhD Student – Cancer Research (Luxembourg Institute of Health)

NEW Research Technician – Melanoma (King’s College London)

Postdoctoral Fellowship – Inner Ear Regeneration (Sunnybrook Research Institute)

Postdoctoral Fellowship – Melanoma (Columbia University)

Research Associate – Cell Competition (University of Bristol)

PhD Student – Cell Biology (Universitatsklinikum Munster)

Postdoctoral Associate – Pathology (Stony Brook University)

PhD Studentship – Melanoma (University of Otago)

Research Fellow – Stem Cell Biology (Brigham & Women’s Hospital)

Postdoctoral Position – Pluripotent Stem Cells (I-STEM)

Assistant Professor – Cancer Biology (University of Pennsylvania)

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