Chronic Stress in Mice Remodels Lymph Vasculature to Promote Tumor Cell Dissemination Scientists showed that chronic stress restructures lymphatic networks within and around tumors to provide pathways for tumor cell escape. They showed that VEGFC derived from tumor cells is required for stress to induce lymphatic remodeling and that this depends on COX2 inflammatory signaling from macrophages. [Nat Commun] Full Article | Press Release The Initiator Methionine tRNA Drives Secretion of Type II Collagen from Stromal Fibroblasts to Promote Tumor Growth and Angiogenesis Researchers generated a mouse expressing additional copies of the tRNAiMet gene (2+tRNAiMet mouse). Growth and vascularization of subcutaneous tumor allografts was enhanced in 2+tRNAiMet mice compared with wild-type littermate controls. Extracellular matrix deposited by fibroblasts from 2+tRNAiMet mice supported enhanced endothelial cell and fibroblast migration. [Curr Biol] Abstract | Press Release Endothelial Cell Survival during Angiogenesis Requires the Pro-Survival Protein MCL1 Scientists investigated the in vivo role of myeloid cell leukemia 1 (MCL1), a pro-survival member of the BCL2 family, in endothelial cell (EC) survival during angiogenesis. EC-specific deletion of Mcl1 resulted in a dose-dependent increase in EC apoptosis in the angiogenic vasculature and a corresponding decline in vessel density. [Cell Death Differ] Abstract Endothelial Cells Decode VEGF-Mediated Ca2+ Signaling Patterns to Produce Distinct Functional Responses Researchers found that VEGF triggers proliferation or migration in endothelial cells by stimulating different calcium signaling patterns. [Sci Signal] Abstract Three-Dimensional Characterization of Mechanical Interactions between Endothelial Cells and Extracellular Matrix during Angiogenic Sprouting Researchers studied the three-dimensional cell-extracellular matrix interactions of endothelial cells that form multicellular structures called sprouts. They analyzed data collected in-situ from angiogenic sprouting experiments and identified the differentiated interaction behavior exhibited by the tip and stalk cells. [Sci Rep] Full Article Tumor-Induced Remote ECM Network Orientation Steers Angiogenesis Investigators use automated sequential microprinting of tumor and endothelial cells in extracellular matrix (ECM) scaffolds to study its mechanical aspects. Quantitative reflection microscopy shows that tumor spheroids induce radial orientation of the surrounding collagen fiber network up to a distance of five times their radius. [Sci Rep] Full Article Advanced Age Decreases Local Calcium Signaling in Endothelium of Mouse Mesenteric Arteries In Vivo Scientists tested the hypothesis that local endothelial cell Ca2+ signaling is impaired with advanced age along with a reduction in internal elastic lamina holes. [Am J Physiol Heart Circ Physiol] Abstract Inhibition of Development of Laser-Induced Choroidal Neovascularization with Suppression of Infiltration of Macrophages in Smad3-Null Mice The authors evaluated the effects of the loss of Smad3 on the development of experimental argon laser-induced choroidal neovascularization in mice. An in vitro angiogenesis model was also used to examine the role of transforming growth factor-β1/Smad3 signaling in vessel-like tube formation by human umbilical vein endothelial cells. [Lab Invest] Abstract Abnormal Correlation of Circulating Endothelial Progenitor Cells and Endothelin-1 Concentration May Contribute to the Development of Arterial Hypertension in Childhood Acute Lymphoblastic Leukemia Survivors Investigators determined the relationship between the rate of circulating endothelial progenitor cells and plasma levels of endothelin-1 with respect to hypertension in childhood acute lymphoblastic leukemia survivors. [Hypertens Res] Abstract |