Endothelial Cell News 2.14 April 24, 2017 | |
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TOP STORYCertain nanoparticles with specific characteristics are able to induce micrometer sized gaps between endothelial cells. This effect is called nanoparticle induced endothelial leakiness (NanoEL). Researchers showed that gold nanoparticles within the range of 10-30 nm are good NanoEL inducing particles. [ACS Nano] Abstract | Graphical Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)Scientists tested in vivo the involvement of disturbed flow, and of neutrophils, hyaluronan, and TLR2 ligation in superficial intimal injury, a process implicated in superficial erosion. In mouse carotid arteries with established intimal lesions tailored to resemble the substrate of human eroded plaques, acute flow perturbation promoted downstream endothelial cell (EC) activation, neutrophil accumulation, EC death and desquamation, and mural thrombosis. [Circ Res] Abstract Investigators generated mice with epidermis-specific deletion of α3β1, α9β1, or both integrins as well as keratinocyte lines expressing these integrin combinations. Consistent with proangiogenic roles for α3β1, α3-null keratinocytes showed reduced paracrine stimulation of endothelial cell migration and survival, and wounds of epidermis-specific α3 knockout mice displayed impaired angiogenesis. [J Cell Biol] Abstract The authors assessed the function of CMTM3, which has been previously identified as highly expressed in Flk1+ endothelial progenitor cells during embryonic development. Using a 3-dimensional coculture of human umbilical vein endothelial cells-GFP and pericytes-RFP, they demonstrated that siRNA-mediated CMTM3 silencing in human umbilical vein endothelial cells impairs angiogenesis. [Arterioscler Thromb Vasc Biol] Abstract MiR-191 Suppresses Angiogenesis by Activation of NF-κB Signaling Scientists identified microRNA-191-5p (miR-191) as a potent inhibitor of blood vessel development. Transfection of human dermal microvascular endothelial cells with miR-191 mimic inhibited their proliferation, migration, and tube formation. [FASEB J] Abstract Fluorescence and Bioluminescence Imaging of Angiogenesis in Flk1–Nano–lantern Transgenic Mice The authors report the generation of a novel transgenic (Tg) mouse that expresses a bioluminescent reporter protein, Nano–lantern, under the control of fetal liver kinase 1 (Flk1). Flk1–Nano–lantern BAC Tg mice recapitulated endogenous Flk1 expression in endothelial cells and lymphatic endothelial cells during development and tumor growth. [Sci Rep] Full Article The results of microarray analysis, real-time PCR and immunohistochemical analyses showed that hairy/enhancer of split homologue-1 (Hes-1) expression was significantly decreased, while that of osteopontin (OPN) was increased, in atherosclerotic plaques. Immunofluorescence results demonstrated that both Hes-1 and OPN were expressed in endothelial cells of neovessels in atherosclerotic plaques. [Sci Rep] Full Article Identification of a Synergistic Interaction between Endothelial Cells and Retinal Pigment Epithelium Using a physiological long-term co-culture system, researchers determined the effect of retinal pigment epithelium-endothelial cell heterotypic interactions on cell survival, behavior and matrix deposition. Their results showed that presence of endothelial cells significantly improves retinal pigment epithelium maturation and function as indicated by the induction of visual cycle-associated genes, accumulation of a Bruch’s membrane-like matrix and increase in retinal pigment epithelium phagocytic activity. [J Cell Mol Med] Abstract | Full Article The authors investigated PML′s role in the regulation of Stat1/2/3 activity. In Pml knockout mice, ablation of Pml largely reduced IFNα angiostatic ability in Matrixgel plug assays. Using molecular tools and primary endothelial cells, they demonstrated that PML positively regulates Stat1 and Stat2 isgylation, a ubiquitination-like protein modification. [J Biol Chem] Abstract NINJ2– A Novel Regulator of Endothelial Inflammation and Activation Investigators hypothesized that ninjurin 2 (NINJ2) may play a role in endothelial inflammation and activation, and regulate the process of atherosclerosis. They demonstrated that NINJ2 can regulate the expression of a panel of genes that are associated with inflammation and atherosclerosis in human vascular endothelial cells. [Cell Signal] Abstract | Graphical Abstract Hemin Causes Lung Microvascular Endothelial Barrier Dysfunction by Necroptotic Cell Death Researchers examined whether hemin can cause lung microvascular endothelial barrier dysfunction. By assessing transendothelial resistance using electrical cell impedance sensing, and by directly measuring trans-monolayer FITC-dextran flux, they found that hemin does cause endothelial barrier dysfunction in a concentration-dependent manner. [Am J Respir Cell Mol Biol] Abstract | |
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REVIEWSRedox Regulation of Gasotransmission in the Vascular System: A Focus on Angiogenesis Scientists focus on the current knowledge of the redox-dependent mechanisms which regulate the generation and activity of gases, with particular reference to their roles in angiogenesis. [Free Radic Biol Med] Full Article | Graphical Abstract Endothelial Cell Malignancies: New Insights from the Laboratory and Clinic The authors describe the major molecular alterations in endothelial cell malignancies and consider emerging opportunities for improving therapeutic efficacy against these rare but deadly tumors. [npj Precis Oncol] Full Article Visit our reviews page to see a complete list of reviews in the endothelial cell research field. | |
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INDUSTRY NEWSCellular Dynamics International Signs Distribution Deal with STEMCELL Technologies Cellular Dynamics International (CDI) announced it has signed a distribution agreement with STEMCELL Technologies. Under the terms of the agreement, STEMCELL Technologies will distribute CDI’s iCell® catalog of products in North America, Europe, and Singapore, with other countries under consideration. [STEMCELL Technologies Inc.] Press Release Strength in the Numbers behind Cancer Research Stacey Finley, a Gabilan Assistant Professor at University of Southern California Viterbi, was awarded the inaugural Leah Edelstein-Keshet Prize from the Society of Mathematical Biology. Finley was selected for critical research she began during her post-doctoral fellowship at Johns Hopkins University in Baltimore, MD. The aim: to develop a mathematical model to understand the role of angiogenesis in cancer. [University of Southern California, Viterbi School of Engineering] Press Release H3 Biomedicine Inc. announced that is has extended its multi-year collaboration with Foundation Medicine, Inc. for the discovery and development of precision medicines in oncology, which was signed in February 2015. H3 and Foundation Medicine will continue to build upon the progress the two companies have made during the collaboration. [H3 Biomedicine Inc.] Press Release | |
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POLICY NEWSWhat Happened at March for Science Events around the World Tens of thousands of people gathered in Washington D.C., and at least 600 other cities around the world, in what may have been one of the largest-ever demonstrations in support of scientific research and evidence-based policymaking. [Nature News] Editorial French Scientists Focus on the Big Political Picture Researchers in France overwhelmingly oppose the far right and can stamp out its rise by turning out to register their disapproval. [Nature News] Editorial
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EVENTSNEW Gordon Research Conferences: Collagen Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Postdoctoral Fellow – Vascular Biology (University Hamburg-Eppendorf) Postdoctoral Research – Endothelial Biology (University of South Florida) Postdoctoral Position – Intersectional Genetics (VIB) Research Assistant Professor – Endothelial and Epithelial Barrier Function (University of Chicago) Postdoctoral Position – Bioinformatics (The National Center for Scientific Research) PhD Student – Brain Vascular Biology (ETH Zurich) Postdoctoral Research Position – Retinal Cell Biology (University of California, Davis) Postdoctoral Fellow – Cardiopulmonary Molecular Biology (Hannover Medical School) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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