Endothelial Cell News 4.04 February 4, 2019 | |
| |
TOP STORYTalin-deficient endothelium showed altered VE-cadherin organization at endothelial cell-cell junctions in vivo. shRNA-mediated knockdown of talin1 expression in cultured endothelial cells led to increased radial actin stress fibers, increased adherens junction width and increased endothelial monolayer permeability measured by electrical cell-substrate impedance sensing. [Circ Res] Abstract | |
| |
PUBLICATIONS(Ranked by impact factor of the journal)Scientists report that liver sinusoidal endothelial cells (LSECs) were specifically targeted and modulated by melittin nanoparticles (α-melittin-NPs). α-melittin-NPs triggered the activation of LSECs and led to dramatic changes of cytokine/chemokine milieu in the liver, which switched the hepatic immunologic environment to the activated state. [Nat Commun] Full Article Investigators report engineered perfusable microvascular constructs, wherein human embryonic stem cell-derived endothelial cells (hESC-ECs) were seeded both into patterned microchannels and the surrounding collagen matrix. In vitro, the hESC-ECs lining the luminal walls readily sprouted and anastomosed with de novo-formed endothelial tubes in the matrix under flow. [Nat Commun] Full Article CCL27/CCL28-CCR10 Chemokine Signaling Mediates Migration of Lymphatic Endothelial Cells CCL27 and CCL28 were expressed in tumor cells with metastatic potential, while their cognate receptor, CCR10, was expressed by lymphatic endothelial cells (LEC) and upregulated by the lymphangiogenic growth factor VEGF-D and the pro-inflammatory cytokine TNF-α. LEC were attracted to both CCL27 and CCL28 in a CCR10-dependent manner. [Cancer Res] Abstract Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2 The authors identified Bmp2 as a downstream target of the calcineurin/NFAT pathway in lung endothelium, potently inhibiting cancer cell growth by stimulating differentiation. They revealed a dual role of calcineurin/NFAT signaling in vascular regression or stabilization and in the tissue-specific production of an angiocrine factor restraining cancer cell outgrowth. [Cell Rep] Full Article | Graphical Abstract Incubation of HUVECs with H2O2 led to cell apoptosis, dysfunction of the nitric oxide systems, the imbalance of calcium homeostasis and energy metabolism featured with increased intracellular free calcium and decreased ATP and downregulated Nrf2, while in the HUVECs pre-treated with 1 μM apirin eugenol ester for 24 hours, the above disorders induced by H2O2 were significantly ameliorated. [Br J Pharmacol] Abstract Researchers observed the angiogenic response of HUVECs in gelatin methacrylate (GelMA) in a concentration-dependent manner. The seeding of HUVECs engineered to express human vascular endothelial growth factor on to the GelMA micro-hydrogel and the subsequent transplantation of the micro-hydrogel into a hindlimb ischemia model effectively attenuated the ischemia condition. [Acta Biomater] Abstract | Graphical Abstract Thrombin had a rapid disruptive effect on endothelial function, but activated protein C (APC) had a slow protective effect. Paralleled by prolonged MAPK 42/44 signaling activation by APC via β-arrestin-2, a lower cleavage rate of PAR-1 for APC than thrombin was quantitatively visualized by bioluminescence video imaging. [Cell Death Dis] Full Article Using total internal reflection fluorescence microscopy, investigators quantified transcytosis of albumin across primary human microvascular endothelial cells from both lung and skin. They then validated the in vitro findings using a tissue-specific knockout mouse model. [Am J Physiol Lung Cell Mol Physiol] Abstract Colloidal Gels with Tunable Mechanomorphology Regulate Endothelial Morphogenesis Endothelial cell (EC) networks were favored in tenuous strand-like microstructure through increased cell-matrix and cell-cell interactions, while compact dense microstructure inhibited the networks. For a given microstructure, as the gel stiffness was increased, the extent of EC network was reduced. [Sci Rep] Full Article KEGG-based pathway analysis identified six significantly enriched pathways of which the p53 pathway was the most affected. No significant enrichment of inflammatory pathways was found, yet, methylglyoxal (MGO) did inhibit VCAM-1 expression in Western blot analysis. Carnosine significantly counteracted MGO-mediated changes in a subset of differentially expressed genes. [Sci Rep] Full Article Subscribe to one of our other 19 science newsletters such as Extracellular Matrix News & Cell Therapy News. | |
| |
REVIEWSThe Role of Fibrinolytic Regulators in Vascular Dysfunction of Systemic Sclerosis Fibrinolytic regulators, such as plasminogen, plasmin, α2-antiplasmin, tissue-type plasminogen activator, urokinase-type plasminogen activator and its receptor, plasminogen activator inhibitor 1, and angiostatin, are considered to play an important role in the maintenance of endothelial homeostasis, and are associated with the endothelial dysfunction of systemic sclerosis. [Int J Mol Sci] Full Article Visit our reviews page to see a complete list of reviews in the endothelial cell research field. | |
| |
INDUSTRY NEWSRoche announced that the European Medicines Agency’s Committee for Medicinal Products for Human Use (CHMP) has recommended the approval of Tecentriq®, in combination with Avastin®, paclitaxel and carboplatin, for the first-line treatment of adults with metastatic non-squamous non-small cell lung cancer. [F. Hoffmann-La Roche Ltd] Press Release Hemostemix Announces Submission of Orphan Drug Designation Application to the FDA Hemostemix Inc. announced that it has submitted an application with the FDA to receive Orphan Drug Designation status for its lead product ACP-01 for the treatment of critical limb ischemia. [Hemostemix Inc.] Press Release AVEO Oncology Announces NDA Timing Update AVEO Oncology announced that it has accepted the recommendation of FDA not to submit a New Drug Application (NDA) for tivozanib with the preliminary overall survival results from the Phase III TIVO-3 trial. [AVEO Pharmaceuticals, Inc.] Press Release Opthea Granted Japanese Patent Covering OPT-302 Opthea Limited announced the grant of Japanese Patent Number 6408492 covering its OPT-302 soluble VEGFR-3 trap’ molecule. The Japanese patent covers both OPT-302 and compositions containing OPT-302 for use in treating disorders associated with aberrant angiogenesis and/or lymphangiogenesis, including eye diseases such are wet age-related macular degeneration and diabetic macular edema. [Opthea (GlobeNewswire, Inc.)] Press Release | |
| |
POLICY NEWSIndian Government to Boost Stipend for Early-Career Scientists The Indian government announced that it will increase the stipend for PhD students who have qualified for a research fellowship by up to 25%. [Nature News] Editorial After Shutdown, NSF Catching Up on Review Panels, Funding Requests When the US government was partially shut down for 35 days starting December 22, work at several government agencies ground to a halt, with a total of 800,000 federal employees furloughed. The National Science Foundation (NSF), one of the sidelined science agencies, is now addressing all that went undone during that time, NSF officials told reporters. [The Scientist] Editorial
| |
EVENTSNEW Keystone Symposia: Cancer Metastasis: The Role of Metabolism, Immunity and the Microenviroment Visit our events page to see a complete list of events in the community.
| |
JOB OPPORTUNITIESNEW Faculty Position – Clinical & Experimental Therapeutics (Augusta University) NEW Postdoctoral Position – Cardiovascular Research (University of Hawaii) Postdoctoral Research Assistant – Endotheial Heterogeneity (University of Oxford) Postdoctoral Fellows – Vascular Immunology & Immunotherapy (Uppsala University) Assistant Professor – Sarcoma & Skeletal-Related Malignancies (UC Davis School of Medicine) Assistant Professor Tenure Track – Epithelial Cell Biology (Boston Children’s Hospital) Postdoctoral Fellowship – Vascular & Valvular Biology (Yale University School of Medicine) Postdoctoral Fellowship – Cardiovascular Sciences (Medical College of Wisconsin) Senior Research Assistant – Genitourinary Medical Oncology (MD Anderson Cancer Center) Assistant/Associate Adjunct Professor – Cardiovascular Medicine (UC Davis) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
| |
Have we missed an important article or publication in Endothelial Cell News? Click here to submit! Comments or suggestions? Submit your feedback here. | |
|