Endothelial Cell News Volume 5.06 | Feb 24 2020

TOP STORY

Caveolae in CNS Arterioles Mediate Neurovascular Coupling

Scienists found that arteriolar endothelial cells, unlike other vascular segments of endothelial cells in the central nervous system, had abundant caveolae. [Nature] Abstract

(Ranked by impact factor of the journal)

Single-Cell Transcriptome Atlas of Murine Endothelial Cells

The authors constructed an atlas of >32,000 single-endothelial cell (EC) transcriptomes from 11 mouse tissues and identified 78 EC subclusters, including Aqp7⁺ intestinal capillaries and angiogenic ECs in healthy tissues. ECs from brain/testis, liver/spleen, small intestine/colon, and skeletal muscle/heart pairwise expressed partially overlapping marker genes. [Cell] Abstract

Generation of Human Endothelium in Pig Embryos Deficient in ETV2

ETV2-null blastocysts were injected with human induced pluripotent stem cells overexpressing the antiapoptotic factor BCL2, transferred to synchronized gilts and analyzed between embryonic day 17 and embryonic day 18. In these embryos, all endothelial cells were of human origin. [Nat Biotechnol] Abstract

Long Noncoding RNA SNHG12 Integrates a DNA-PK–Mediated DNA Damage Response and Vascular Senescence

Investigators found that the evolutionarily conserved lncRNA small nucleolar host gene-12 (SNHG12) was highly expressed in the vascular endothelium and decreases during lesion progression. SNHG12 knockdown accelerated atherosclerotic lesion formation by 2.4-fold in Ldlr^−/− mice by increased DNA damage and senescence in the vascular endothelium, independent of effects on lipid profile or vessel wall inflammation. [Sci Transl Med] Abstract

Vascular Endothelium-Targeted Sirt7 Gene Therapy Rejuvenates Blood Vessels and Extends Life Span in a Hutchinson-Gilford Progeria Model

Lmna^f/f;TC mice with progerin expression induced by Tie2-Cre exhibited defective microvasculature and neovascularization, accelerated aging, and shortened life span. Single-cell transcriptomic analysis of murine lung endothelial cells revealed a substantial up-regulation of inflammatory response. [Sci Adv] Full Article

Epithelial VEGFA Specifies a Distinct Endothelial Population in the Mouse Lung

Researchers showed that VEGFA from the epithelium was required for a distinct endothelial cell population in the mouse lung. [Dev Cell] Full Article | Graphical Abstract

Glioma-Derived miRNA-Containing Extracellular Vesicles Induce Angiogenesis by Reprogramming Brain Endothelial Cells

Glioblastoma stem cell-derived extracellular vesicles (GSC-EVs) and normal vascular growth factors stimulated highly distinct gene regulatory responses that converged on angiogenesis. The response to GSC-EVs showed a footprint of post-transcriptional gene silencing by EV-derived miRNAs. [Cell Rep] Full Article | Graphical Abstract

iPSC-Derived Endothelial Cells Affect Vascular Function in a Tissue-Engineered Blood Vessel Model of Hutchinson-Gilford Progeria Syndrome

To elucidate the effects of progerin on endothelial cells, scientists prepared tissue-engineered blood vessels using induced pluripotent stem cell-derived smooth muscle cells and endothelial cells (viECs) from Hutchinson-Gilford progeria syndrome (HGPS) patients. HGPS viECs aligned with flow but exhibited reduced flow-responsive gene expression and altered NOS3 levels. [Stem Cell Reports] Full Article

Comprehensive Transcriptomic Profiling Reveals SOX7 as an Early Regulator of Angiogenesis in Hypoxic Human Endothelial Cells

Researchers observed that hypoxia modulated the expression levels of about one third of the endothelial cell transcriptome. Expression of the gene encoding the developmental transcription factor SRY-box transcription factor 7 (SOX7) rapidly and transiently increased during hypoxia. [J Biol Chem] Abstract | Full Article

Human Primary Endothelial Label-Free Biochip Assay Reveals Unpredicted Functions of Plasma Serine Proteases

Investigators screened a set of plasma serine proteases as possible endothelial cell activators, and identified the endothelial cell activating function of three important serine proteases – namely kallikrein, C1r and mannan-binding lectin-associated serine-protease 2 – and verified these results in well-established functional assays. [Sci Rep] Full Article

Subscribe to one of our other 19 science newsletters such as Extracellular Matrix News & Cell Therapy News.

Lymphatic Endothelial Cells of the Lymph Node

Recent analyses in mice and humans have revealed unexpected diversity in the lymphatic endothelial cells, which form the distinct regions of the sinuses. As a semipermeable barrier, the lymphatic endothelial cells regulate the sorting of lymph-borne antigens to the lymph node parenchyma and can themselves serve as antigen-presenting cells. [Nat Rev Immunol] Abstract

Visit our reviews page to see a complete list of reviews in the endothelial cell research field.

Baylor Researchers Receive Nearly $2.7 Million in CPRIT Grants

Baylor College of Medicine has been awarded nearly $2.7 million by the Cancer Prevention and Research Institute of Texas (CPRIT) to support new cancer research. [Baylor College of Medicine] Press Release

Novartis Receives EC Approval for Beovu^®, a Next-Generation Anti-VEGF Treatment for Wet AMD, a Leading Cause of Blindness Worldwide

Novartis announced the European Commission (EC) has approved Beovu^® injection for the treatment of wet age-related macular degeneration (AMD). [Novartis] Press Release

‘The Disruption Is Enormous.’ Coronavirus Epidemic Snarls Science Worldwide

Normal daily life has come to a virtual standstill in large parts of China as a result of the epidemic of COVID-19—and so has science. Universities across the country remain closed; access to labs is restricted, projects have been mothballed, field work interrupted, and travel severely curtailed. [ScienceInsider] Editorial

White House Formally Invites Public Comment on Open-Access Policies

The White House issued a notice Wednesday seeking comment on its effort to enhance public access to federally funded research. It’s an old idea creating new controversy. White House Office of Science and Technology Policy Director Kelvin Droegemeier is pushing back against publishers that in December said the administration was quietly pursuing an executive order to require immediate free distribution of taxpayer funded research. [ScienceInsider] Editorial

Has the UK Science Minister Been Demoted?

A government reshuffle in the United Kingdom has born another new science minister – a position that has seen a revolving door of appointees, firings and resignations in recent years. The latest appointment also comes with an apparent political demotion for the role, which expert observers say is in conflict with a government that appears to have put research and innovation at the heart of its agenda. [Nature News] Editorial

NEW Adhesion Across Scales: From Molecules to Morphogenesis
June 28-July 3, 2020
Manchester, New Hampshire, United States

Visit our events page to see a complete list of events in the community.

NEW Research Fellow – Vascular Biology (Queen’s University Belfast)

NEW Postdoctoral Fellowship – Endothelial Injury in Type 2 Diabetes (Karolinska Institutet)

NEW PhD Candidate – Autoimmunity and Kidney Diseases (Radboud University Medical Centre)

NEW Career Scientist – Neurosurgery and Regenerative Medicine (Mayo Clinic)

Postdoctoral Researcher – Neurodegeneration and Vascular Functions (Icahn School of Medicine at Mount Sinai)

Postdoctoral Researcher – Microvascular Injury (University Hospital Carl Gustav Carus Dresden)

Postdoctoral Researcher – Human Atherosclerosis (Karolinska Institutet)

PhD Position – Aging (Lausanne University Hospital)

Postdoctoral Fellow – Vascular Dysfunction in Neurodegeneration (Lund University)

Post-Doctoral Fellow – Metabolic Regulation of Vascular Formation (Oklahoma Medical Research Foundation)

Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.

Have we missed an important article or publication in Endothelial Cell News? Click here to submit!

Comments or suggestions? Submit your feedback here.