Dppa3 Expression Is Critical for Generation of Fully Reprogrammed iPS Cells and Maintenance of Dlk1-Dio3 Imprinting Scientists show that germ-cell marker Dppa3 is present only in low-grade chimera forming induced pluripotent stem cells (iPSCs) and high-grade chimera production competent iPSCs (hg-iPSCs), and that induction with exogenous Dppa3 enhances reprogramming kinetics, generating all hg-iPSCs, similar to vitamin C. [Nat Commun] Full Article hnRNP K Coordinates Transcriptional Silencing by SETDB1 in Embryonic Stem Cells Investigators identified the transcription co-factor heterogeneous nuclear ribonucleoprotein K (hnRNP K) as a novel binding partner of the SETDB1/KAP1 complex in mouse embryonic stem cells and show that hnRNP K is required for endogenous retrovirus silencing. [PLoS Genet] Full Article Comparative Computational Analysis of Pluripotency in Human and Mouse Stem Cells Researchers developed a pipeline to reanalyze transcriptomics data sets that describe both states, naive and primed pluripotency, in human and mouse. The pipeline consists of identifying regulated start-ups/shut-downs in terms of molecular interactions, followed by functional annotation of the genes involved and aggregation of results across conditions, yielding sets of mechanisms that are consistently regulated in transitions towards similar states of pluripotency. [Sci Rep] Full Article Embryonic Stem Cell Differentiation Requires Full Length Chd1 Scientists studied the effects of a partial deletion of the gene encoding the chromatin remodeling factor Chd1 that generates an N-terminally truncated version of Chd1 in mouse embryonic stem cells in vitro as well as in vivo. [Sci Rep] Full Article Functional Comparison of Induced Pluripotent Stem Cell- and Blood-Derived GPIIbIIIa Deficient Platelets Researchers generated a human induced pluripotent stem cell (hiPSC) model of the primary platelet disease Glanzmann thrombasthenia, characterized by dysfunction of the integrin receptor GPIIbIIIa, and compared side-by-side healthy and diseased hiPSC-derived platelets with peripheral blood platelets. [PLoS One] Full Article Derivation of Hair-Inducing Cell from Human Pluripotent Stem Cells The authors directed human embryonic stem cells (hESCs) to generate first neural crest cells and then hair-inducing dermal papillae (DP) -like cells in culture. They showed that hESC-derived DP-like cells express markers typically found in adult human DP cells and are able to induce hair follicle formation when transplanted under the skin of immunodeficient NUDE mice. [PLoS One] Full Article | Press Release Gene and MicroRNA Profiling of Human Induced Pluripotent Stem Cell-Derived Endothelial Cells Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were differentiated into endothelial cells (ECs). Then the endothelial-related gene profiling and miRNA profiling of hiPSCs, hESCs, hiPSCs derived endothelial cells, hESC derived endothelial cells and human umbilical vein endothelial cells were compared using RT-PCR Array. [Stem Cell Rev] Abstract Synergistic Effects of Hypoxia and Morphogenetic Factors on Early Chondrogenic Commitment of Human Embryonic Stem Cells in Embryoid Body Culture Scientists investigated the interactive effects of controlled variation of oxygen tension and chondrocyte-secreted morphogenetic factors on chondrogenic differentiation of human embryonic stem cells in the embryoid body format. [Stem Cell Rev] Abstract |