ESC & iPSC News Volume 10.19 | May 20 2015

A Primary Role for the Tsix lncRNA in Maintaining Random X-Chromosome Inactivation
Researchers found that Xist is stably repressed on the X^ΔTsix in both sexes in undifferentiated epiblast cells in vivo and in vitro, resulting in stochastic X-inactivation in females despite Tsix-heterozygosity. [Cell Rep] Full Article | Graphical Abstract

A Method for Human Teratogen Detection by Geometrically Confined Cell Differentiation and Migration
Researchers spatially directed mesoendoderm differentiation, epithelial-mesenchymal transition and the ensuing cell migration in micropatterned human pluripotent stem cell colonies to collectively form an annular mesoendoderm pattern. [Sci Rep] Full Article

Development of a Monitoring Method for Nonlabeled Human Pluripotent Stem Cell Growth by Time-Lapse Image Analysis
Using a time-lapse imaging system, investigators developed a method to determine the growth of human pluripotent stem cells from non-labeled live cell phase-contrast images without damaging these cells. [Stem Cells Transl Med] Abstract

Directed Differentiation of Oligodendrocyte Progenitor Cells From Mouse Induced Pluripotent Stem Cells
The authors described a standardized, defined protocol that reliably directs the differentiation of mouse induced pluripotent stem cells to generate high yields of oligodendrocyte progenitor cells that are capable of maturing into oligodendrocytes. [Cell Transplant] Abstract

Genome-Wide Definition of Promoter and Enhancer Usage during Neural Induction of Human Embryonic Stem Cells
Scientists combined high-throughput identification of transcription start sites with genome-wide profiling of histones modifications to map active promoters and enhancers in embryonic stem cells induced to neuroepithelial-like stem cells. [PLoS One] Full Article

HSA21 Single-Minded 2 (SIM2) Binding Sites Co-Localize with Super-Enhancers and Pioneer Transcription Factors in Pluripotent Mouse ES Cells
Researchers aimed to explore the functional capacity of SIM2 by mapping its DNA binding sites in mouse embryonic stem (ES) cells. Analysis of the SIM2 target genes confirmed the importance of SIM2 in developmental and neuronal processes and indicated that SIM2 may be a master transcription regulator. [PLoS One] Full Article

Directed Myogenic Differentiation of Human Induced Pluripotent Stem Cells
Scientists introduced a myogenic differentiation protocol mediated by inducible transcription factor expression that reproducibly and efficiently drives human induced pluripotent stem cells into myocytes. [Methods Mol Biol] Abstract

iPSCs as a Major Opportunity to Understand and Cure Age-Related Diseases
The authors describe the hallmarks of cellular senescence before presenting how they reprogrammed aged and senescent cells into induced pluripotent stem cells and obtained rejuvenated re-differentiated cells. [Biogerontology] Abstract

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UB and Roswell Park Receive $1.85 MIllion Grant to Launch Stem Cell Research Program
University at Buffalo and Roswell Park Cancer Institute researchers have been awarded a $1.85 million grant to create an interdisciplinary stem cell research training program. [University of Buffalo] Press Release

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Steve Campana, Canadian Biologist, ‘Disgusted’ with Government Muzzling
A recently retired Fisheries and Oceans Canada biologist says the muzzling of federal government scientists is worse than anyone can imagine. Steve Campana, known for his expertise on everything from great white sharks to porbeagles and Arctic trout, says the atmosphere working for the federal government is toxic. [Canadian Broadcasting Corporation] Editorial

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Scientist – Human Pluripotent Stem Cell Biology (STEMCELL Technologies Inc.)

Postdoctoral Researcher- Systems Biology of X-Inactivation (Max Planck Institute for Molecular Genetics)

Postdoctoral Position – Cell Replacement Therapy for Parkinson’s Disease (Memorial Sloan Kettering Cancer Center)

Postdoctoral Research Fellow – Stem Cell Disease Modeling (Nanyang Technological University)

Postdoctoral Position – Stem Cell Biology, Epigenetics & Cancer (Albert Einstein College of Medicine of Yeshiva University)

Stem Cell Culture Scientist (Paramount Recruitment)

Postdoctoral Position – Induced Pluripotent Stem Cells to Study Metabolism and Gene Expression Regulating Neural Tube Development (Joslin Diabetes Center)

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