Transient Acquisition of Pluripotency during Somatic Cell Transdifferentiation with iPSC Reprogramming Factors Scientists showed that the vast majority of reprogrammed cardiomyocytes or neural stem cells obtained from mouse fibroblasts by Oct4, Sox2, Klf4 and c-Myc-induced ‘transdifferentiation’ pass through a transient pluripotent state, and that their derivation was molecularly coupled to induced pluripotent stem cells formation mechanisms. [Nat Biotechnol] Abstract Epigenetic Regulation of Phosphodiesterases 2A and 3A Underlies Compromised β-Adrenergic Signaling in an iPSC Model of Dilated Cardiomyopathy The authors found that the β-adrenergic agonist isoproterenol induced mature β-adrenergic signaling in induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) but that this pathway was blunted in dilated cardiomyopathy iPSC-CMs. [Cell Stem Cell] Abstract | Graphical Abstract The Nucleoporin Nup153 Regulates Embryonic Stem Cell Pluripotency through Gene Silencing Investigators showed that depletion of Nup153 in mouse embryonic stem cells caused the derepression of developmental genes and induction of early differentiation. This loss of stem cell identity was not associated with defects in the nuclear import of key pluripotency factors. [Genes Dev] Abstract Generation of Fertile Offspring from Kitw/Kitwv Mice through Differentiation of Gene Corrected Nuclear Transfer Embryonic Stem Cells Researchers derived embryonic stem cells from cloned embryos (ntESCs) that were created by nuclear transfer of Kitw/Kitwv somatic cells. Then they generated gene-corrected ntESCs using TALEN-mediated gene editing. [Cell Res] Abstract A Cytotoxic Antibody Recognizing Lacto-N-Fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells The authors generated a mouse monoclonal antibody (R-17F, IgG1 subtype) specific to hiPS/embryonic stem cells by using a hiPS cell line as an antigen. [J Biol Chem] Abstract | Full Article Generation of Human iPSC from Cells of Fibroblastic and Epithelial Origin by Means of the oriP/EBNA-1 Episomal Reprogramming System Researchers performed a side-by-side comparison of induced pluripotent stem cell (iPSC) colony forming efficiencies in fibroblasts and epithelial cells transiently transfected with episomal plasmids, and demonstrated that iPSC generation efficiency was highest when donor samples were derived from epithelial cells. [Stem Cell Res Ther] Full Article Combined Negative Effect of Donor Age and Time in Culture on the Reprogramming Efficiency into Induced Pluripotent Stem Cells Human fibroblasts from 11 different donors of variable age were reprogrammed by ectopic expression of reprogramming factors. Although all fibroblasts gave rise to induced pluripotent stem cell colonies, the reprogramming efficiency correlated negatively and declined rapidly with increasing donor age. [Stem Cell Res] Full Article Tetraploid Embryonic Stem Cells Maintain Pluripotency and Differentiation Potency into Three Germ Layers Investigators established tetraploid embryonic stem cells (TESCs) produced from the inner cell masses of tetraploid blastocysts using electrofusion of two-cell embryos in mice and studied the developmental potential of TESCs. They demonstrated that TESCs possessed essential pluripotency and differentiation potency to form teratomas, which differentiated into the three germ layers, including diploid embryonic stem cells. [PLoS One] Full Article Tbx3 Controls Dppa3 Levels and Exit from Pluripotency toward Mesoderm Scientists showed that mouse embryonic stem cells (mESC) existed in an alternate stable pluripotent state in the absence of Tbx3. In-depth transcriptome analysis of this mESC state revealed Dppa3 as a direct downstream target of Tbx3. [Stem Cell Rep] Full Article | Graphical Abstract |