Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency The authors constructed and characterized a clonal, inducible human reprogramming system that provided a reliable source of cells at any stage of the process. This system enabled integrative transcriptional and epigenomic analysis across the human reprogramming timeline at high resolution. [Cell] Full Article | Graphical Abstract Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells Scientists showed that human induced pluripotent stem cells differentiate into incipient mesoderm-like cells, which robustly generated human primordial germ cell-like cells that can be purified using the surface markers EpCAM and INTEGRINα6. [Cell Stem Cell] Abstract | Graphical Abstract Generation of Stomach Tissue from Mouse Embryonic Stem Cells Researchers established a method for stomach differentiation from embryonic stem cells by inducing mesenchymal Barx1, an essential gene for in vivo stomach specification from gut endoderm. [Nat Cell Biol] Abstract SetDB1 Modulates PRC2 Activity at Developmental Genes Independent of H3K9 Trimethylation in Mouse ES Cells Researchers characterized genome-wide SETDB1 binding and H3K9 trimethylation profiles in mouse embryonic stem cells and uncovered two distinct classes of SETDB1 binding sites, termed solo and ensemble peaks. [Genome Res] Abstract Jarid2 Coordinates Nanog Expression and PCP/Wnt Signaling Required for Efficient ESC Differentiation and Early Embryo Development Scientists showed that Jarid2−/− embryonic stem cells (ESCs) express constitutively high levels of Nanog but reduced PCP signaling components Wnt9a, Prickle1, and Fzd2 and lowered β-catenin activity. Depletion of Wnt9a/Prickle1/Fzd2 from wild-type ESCs or overexpression of Nanog largely phenocopies these cellular defects. [Cell Rep] Full Article | Graphical Abstract | Press Release Single-Step Generation of Conditional Knockout Mouse Embryonic Stem Cells Investigators developed a reporter system to enrich for cells with engineered nuclease-assisted homologous recombination events. Using this system in mouse embryonic stem cells, they achieved single-step biallelic and seamless integration of two loxP sites for Cre recombinase-mediated inducible gene knockout, as well as biallelic endogenous gene tagging with high efficiency. [Cell Rep] Full Article | Graphical Abstract Single Cell Analysis Reveals Concomitant Transcription of Pluripotent and Lineage Markers during the Early Steps of Differentiation of Embryonic Stem Cells The author used immuno-staining and single molecule RNA FISH to assess changes in the expression of the well-known pluripotency-associated gene Pou5f1 (also known as Oct4) and early differentiation markers Sox1 and T-brachyury in single cells during the early steps of differentiation of mouse embryonic stem cells. [Stem Cells] Abstract Uncovering the Role of Hypermethylation by CTG Expansion in Myotonic Dystrophy Type 1 Using Mutant Human Embryonic Stem Cells Using 14 different myotonic dystrophy type 1-affected human embryonic stem cell lines, scientists characterized a differentially methylated region near CTGs. [Stem Cell Reports] Full Article | Graphical Abstract Retinoic Acid Induces Embryonic Stem Cell Differentiation by Altering Both Encoding RNA and MicroRNA Expression Using mRNA microarray analysis, investigators showed that retinoic acid repressed pluripotency-associated genes while activating ectoderm markers in mouse embryonic stem cells. [PLoS One] Full Article |