Functional Correction of Large Factor VIII Gene Chromosomal Inversions in Hemophilia a Patient-Derived iPSCs Using CRISPR-Cas9 Researchers derived induced pluripotent stem cells (iPSCs) from patients with inversion genotypes and used CRISPR-Cas9 nucleases to revert these chromosomal segments back to the WT situation. [Cell Stem Cell] Abstract | Graphical Abstract Generation of Vascular Endothelial and Smooth Muscle Cells from Human Pluripotent Stem Cells Researchers found that GSK3 inhibition and BMP4 treatment rapidly committed pluripotent cells to a mesodermal fate and subsequent exposure to VEGF-A or PDGF-BB resulted in the differentiation of either endothelial or vascular smooth muscle cells, respectively. [Nat Cell Biol] Abstract NF-κB Activation Impairs Somatic Cell Reprogramming in Aging Investigators explored the regulatory mechanisms that constitute age-associated barriers, through derivation of induced pluripotent stem cells (iPSCs) from individuals with premature or physiological aging. They demonstrated that NF-κB activation blocks the generation of iPSCs in aging. [Nat Cell Biol] Abstract Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells Scientists report a genetic screen for silencing factors in X chromosome inactivation using haploid mouse embryonic stem cells that carry an engineered selectable reporter system. [Cell Rep] Full Article | Graphical Abstract CHD1L Regulated PARP1-Driven Pluripotency and Chromatin Remodeling during the Early-Stage Cell Reprogramming Researchers demonstrated that CHD1L, a DNA helicase, possesses chromatin remodeling activity and interacts with PARP1/PARylation in regulating pluripotency during reprogramming. [Stem Cells] Abstract A Pre-Screening FISH-Based Method to Detect CRISPR/Cas9 Off-Targets in Mouse Embryonic Stem Cells Scientists showed that a simple fluorescence in situ hybridization (FISH)-based strategy allows the detection and the frequency evaluation of non-specific integrations of a given plasmid. FISH analysis revealed that these integrations do not match the software predicted off-target loci. [Sci Rep] Full Article Prediction of Drug-Induced Nephrotoxicity and Injury Mechanisms with Human Induced Pluripotent Stem Cell-Derived Cells and Machine Learning Methods Researchers developed a rapid 1-step protocol for the differentiation of human induced pluripotent stem cells into proximal tubular-like cells. [Sci Rep] Full Article Generation of Naivetropic Induced Pluripotent Stem Cells from Parkinson’s Disease Patients for High Efficiency Genetic Manipulation and Disease Modeling Scientists converted patient-specific induced pluripotent stem cells from the primed state to a naivetropic state by DOX-induced expression of transgenes (Oct4, Sox2, Klf4, c-Myc and Nanog) and the use of 2iL. [Stem Cells Dev] Abstract AF9 Promotes hESC Neural Differentiation through Recruiting TET2 to Neurodevelopmental Gene Loci for Methylcytosine Hydroxylation Investigators showed that AF9 is necessary and sufficient for human embryonic stem cell (hESC) neural differentiation and neurodevelopmental gene activation. [Cell Disc] Full Article |