Network Plasticity of Pluripotency Transcription Factors in Embryonic Stem Cells Investigators used reporter mouse embryonic stem cell lines allowing non-invasive quantification of Nanog or Oct4 protein levels and continuous long-term single-cell tracking and quantification over many generations to reveal diverse transcription factor protein expression dynamics. [Nat Cell Biol] Abstract Srebp-1 Interacts with C-Myc to Enhance Somatic Cell Reprogramming Researchers found that overexpression of sterol regulatory element binding protein-1 (Srebp-1), a transcriptional factor required for lipid homeostasis, enhances reprogramming efficiency, while knockdown or pharmaceutical inhibition of Srebp-1 is inhibitory. [Stem Cells] Abstract Structural Changes in N-Glycans on Induced Pluripotent Stem Cells Differentiating Toward Cardiomyocytes Scientists focused on N-glycans produced in human induced pluripotent stem cells (iPSCs) and iPSC-derived cardiomyocytes to investigate their change on cardiomyogenic differentiation in vitro. [Stem Cells Transl Med] Abstract | Full Article Dynamic and Social Behaviors of Human Pluripotent Stem Cells Scientists used non-invasive time-lapse imaging to continuously examine human pluripotent stem cell maintenance and differentiation and to predict cell viability and fate. [Sci Rep] Full Article Edges of Human Embryonic Stem Cell Colonies Display Distinct Mechanical Properties and Differentiation Potential The authors examined the differentiation process in adherent colonies of human embryonic stem cells. Live imaging of the differentiation process revealed that cells on the outer edge of the undifferentiated colony begin to differentiate first and remain on the perimeter of the colony to eventually form a band of differentiation. [Sci Rep] Full Article Activin/Nodal Signaling Supports Retinal Progenitor Specification in a Narrow Time Window during Pluripotent Stem Cell Neuralization Researchers used chemically defined media to induce in vitro differentiation of mouse embryonic stem cells (ESCs) toward eye field fates. [Stem Cell Reports] Full Article Glutamine Contributes to Maintenance of Mouse Embryonic Stem Cell Self-Renewal through PKC-Dependent Downregulation of HDAC1 and DNMT1/3a Scientists investigated the effect of glutamine on regulation of mouse embryonic stem cell self-renewal and related signaling pathways. [Cell Cycle] Abstract Changes in Laminin Expression Pattern during Early Differentiation of Human Embryonic Stem Cells Researchers investigated the potential changes in laminin expression during early human embryonic stem cell (hESC) differentiation induced by retinoic acid. They found that laminin-511 but not -521 accumulated in the committed cells during early steps of hESC differentiation. [PLoS One] Abstract Noggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells Researchers demonstrated that early noggin exposure may play a specific role in the directed differentiation of dopaminergic cells from human embryonic stem cells. [PLoS One] Full Article |