Tgif1 Counterbalances the Activity of Core Pluripotency Factors in Mouse Embryonic Stem Cells Researchers report the direct repression of core pluripotency factors by Tgif1, a previously known terminal repressor of TGFβ/activin/nodal signaling. [Cell Rep] Full Article | Graphical Abstract The Knockdown of H19lncRNA Reveals Its Regulatory Role in Pluripotency and Tumorigenesis of Human Embryonic Carcinoma Cells To study the role of H19 in oncogenesis and pluripotency, investigators down-regulated H19 expression in vitro and in vivo in pluripotent human embryonic carcinoma and embryonic stem cells. [Oncotarget] Full Article Cdh2 Stabilizes FGFR1 and Contributes to Primed-State Pluripotency in Mouse Epiblast Stem Cells To understand the function of cadherin 2 (Cdh2) in mouse epiblast stem cells (mEpiESCs), researchers compared the expression of pluripotency-related genes in mEpiSCs and mouse embryonic stem cells after either Cdh2 knockdown or Cdh2 over-expression. [Sci Rep] Full Article OCT4 Coordinates with WNT Signaling to Pre-Pattern Chromatin at the SOX17 Locus during Human ES Cell Differentiation into Definitive Endoderm Investigators demonstrated that the pluripotency gene OCT4 has a role in regulating differentiation via Wnt signaling. OCT4 expression levels in human embryonic stem (ES) cells increased transiently during the first 24 hours of in vitro differentiation, with OCT4 occupancy increasing at endoderm regulators such as SOX17 and FOXA2. [Stem Cell Reports] Full Article Cyclin E1 Plays a Key Role in Balancing between Totipotency and Differentiation in Human Embryonic Cells The authors investigated if Cyclin E1 plays a role in human embryogenesis, in particular during the early developmental stages characterized by a short cell cycle. [Mol Hum Reprod] Abstract Transcriptome Characteristics and X-Chromosome Inactivation Status in Cultured Rat Pluripotent Stem Cells Scientists showed that reprogramming of rat somatic cells to a pluripotent state was accompanied by X-chromosome reactivation. [Stem Cells Dev] Abstract Microgravity Reduces the Differentiation and Regenerative Potential of Embryonic Stem Cells The authors found that exposure to microgravity for 15 days inhibits mouse embryonic stem cell differentiation and expression of terminal germ layer lineage markers in embryoid bodies. [Stem Cells Dev] Abstract Embryonic Stem Cell Growth Factors Regulate eIF2α Phosphorylation The authors found abundant phosphorylated-eIF2α (P-eIF2α) in both pluripotent mouse and human embryonic stem cells (ESCs), but little P-eIF2α in ESCs triggered to differentiate. [PLoS One] Full Article |