Coordination of m6A mRNA Methylation and Gene Transcription by ZFP217 Regulates Pluripotency and Reprogramming Scientists showed that the chromatin-associated zinc finger protein 217 (ZFP217) coordinates epigenetic and epitranscriptomic regulation. ZFP217 interacted with several epigenetic regulators, activated the transcription of key pluripotency genes, and modulated N6-methyladenosine (m6A) deposition on their transcripts by sequestering the enzyme m6A methyltransferase-like 3. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Histone Demethylase Expression Enhances Human Somatic Cell Nuclear Transfer Efficiency and Promotes Derivation of Pluripotent Stem Cells Investigators showed that overexpression of a related histone H3 lysine 9 trimethylation (H3K9me3) demethylase KDM4A improves human somatic cell nuclear transfer (SCNT), and that, as in mice, H3K9me3 in the human somatic cell genome is an SCNT reprogramming barrier. [Cell Stem Cell] Abstract | Graphical Abstract Efficient Delivery of Nuclease Proteins for Genome Editing in Human Stem Cells and Primary Cells The authors describe procedures for implementing protein-based genome editing in human embryonic stem cells and primary cells. [Nat Protoc] Abstract TRIM28 Controls Genomic Imprinting through Distinct Mechanisms during and after Early Genome-wide Reprogramming By analyzing maternal, zygotic, maternal-zygotic, and conditional Trim28 mutants, researchers found that the transcription factor TRIM28 controls genomic imprinting through distinct mechanisms at different developmental stages. During early genome-wide reprogramming, both maternal and zygotic TRIM28 are required for the maintenance of methylation at germline imprints. [Cell Rep] Full Article | Graphical Abstract Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells Researchers showed that the efficiency and kinetics of induced pluripotent stem cells reprogramming are dramatically improved by the combined expression of Jarid2 and genes encoding its associated proteins. [Stem Cells] Abstract Stimulation of Vasculogenesis and Leukopoiesis of Embryonic Stem Cells by Extracellular Transfer RNA and Ribosomal RNA Scientists investigated the impact of extracellular ribonucleic acid, especially transfer RNA, on vasculogenesis and leukopoiesis of mouse embryonic stem cells [Free Radic Biol Med] Full Article Adrenergic DNA Damage of Embryonic Pluripotent Cells via β2 Receptor Signaling Researchers revealed that adrenergic stress mediators cause DNA damage of embryonic pluripotent cells through the β2 adrenergic receptor/adenylate cyclase/cAMP/PKA signaling pathway. The adrenergic stress agonists adrenaline, noradrenaline, and isoprenaline caused DNA damage and apoptosis of embryonic stem cells and embryonal carcinoma stem cells. [Sci Rep] Full Article Three-Dimensional Bioprinting of Embryonic Stem Cells Directs Highly Uniform Embryoid Body Formation Extrusion-based 3D bioprinting technology was utilized for biofabricating embryonic stem cells into a three-dimensional cell-laden construct. [Biofabrication] Full Article | Press Release Rapidly Generating Knockout Mice from H19-Igf2 Engineered Androgenetic Haploid Embryonic Stem Cells Investigators report the high-frequency generation of healthy, fertile mice from H19-Igf2 imprinting-locus modified androgenetic haploid embryonic stem cells, which maintained normal paternal imprinting and pluripotency. [Cell Disc] Full Article |