ESC & iPSC News Volume 10.43 | Nov 18 2015

Generation of Functional Hippocampal Neurons from Self-Organizing Human Embryonic Stem Cell-Derived Dorsomedial Telencephalic Tissue
The authors showed the generation of functional hippocampal granule- and pyramidal-like neurons from self-organizing dorsomedial telencephalic tissue using human embryonic stem cells. [Nat Commun] Full Article

Fas-Activated Mitochondrial Apoptosis Culls Stalled Embryonic Stem Cells to Promote Differentiation
Scientists showed that Bax^−/−Bak^−/− murine embryonic stem cells display defects during the exit from pluripotency, both in culture and during teratoma formation. [Curr Biol] Abstract | Graphical Abstract

Generation of an Expandable Intermediate Mesoderm Restricted Progenitor Cell Line from Human Pluripotent Stem Cells
Scientists used a cellular microarray technology to identify a defined and optimized culture condition that supports the derivation and propagation of a cell population with mesodermal properties. [eLife]
Full Article | Press Release

Cellular Heterogeneity in the Level of mtDNA Heteroplasmy in Mouse Embryonic Stem Cells
Scientists analyzed cellular heterogeneity in embryonic stem cells derived from a polymorphic mouse model containing NZB and BALB mtDNA genotypes. [Cell Rep] Full Article | Graphical Abstract

Improvement in Mouse iPSC Induction by Rab32 Reveals the Importance of Lipid Metabolism during Reprogramming
By testing the efficiency of induced pluripotent stem cell (iPSC) generation using Oct4, Sox2, Klf4 plus one additional gene, investigators found that Rab32 improved reprogramming efficiency. [Sci Rep] Full Article

Dzip3 Regulates Developmental Genes in Mouse Embryonic Stem Cells by Reorganizing 3D Chromatin Conformation
The authors tested whether known H2A ubiquitin ligases and deubiquitylases are involved in mouse embryonic stem cell regulation and discovered that Dzip3, the E3 ligase of H2AK119, represses differentiation-inducible genes, as does Ring1B. [Sci Rep] Full Article

Generation of a High Number of Healthy Erythroid Cells from Gene-Edited Pyruvate Kinase Deficiency Patient-Specific Induced Pluripotent Stem Cells
Investigators generated pyruvate kinase deficiency (PKD) induced pluripotent stem cells from peripheral blood mononuclear cells of PKD patients by non-integrative Sendai viral vectors. [Stem Cell Reports] Full Article

Selective Targeting of the BRG/PB1 Bromodomains Impairs Embryonic and Trophoblast Stem Cell Maintenance
The authors showed that exposure of embryonic stem cells to PFI-3 led to deprivation of stemness and deregulated lineage specification. [Sci Adv] Full Article

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Connecticut Bioscience Innovation Fund Approves $1 Million in Funding for Two Healthcare IT Companies
Connecticut Innovations announced that the Connecticut Bioscience Innovation Fund has approved $1 million in funding to support bioscience innovations during the latest review of project applications. [BioInnovation CT.] Press Release

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Data Check: How U.S. Budget Deal Turns $30 Billion into $3 Billion
Get your money this year—because federal spending in 2017 may feel very tight. That message for U.S. scientists is buried in the new two-year budget agreement that President Barack Obama signed. [ScienceInsider] Editorial

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NEW Postdoctoral Fellow and Research Scientist – Epigenomics and Systems Biology (Icahn School of Medicine at Mount Sinai)

Postdoctoral Fellow – Cardiac Regeneration (Duke University)

Postdoctoral Position – Modeling and Treatment of Human Skeletal Muscle Diseases (Duke University)

Team Leader Position – Developmental Biology (Institut de Biologie Paris-Seine)

Postdoctoral Position – Neuroscience/Stem Cell Research (University of California, Davis)

Postdoctoral Position – Tissue Engineering and Regenerative Medicine (Northwestern University)

Postdoctoral Fellow – iPSC Research (Icahn School of Medicine at Mount Sinai)

Postdoctoral Position – Biomaterials for Cell Reprogramming (Institute of Tissue Regeneration Engineering)

Assistant/Associate/Full Professor – Biomedical Engineering (Worcester Polytechnic Institute)

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