JNK/SAPK Signaling Is Essential for Efficient Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells Investigators found that key components of the JNK/SAPK signaling pathway increase expression as early as day three of the reprogramming process and continue to rise in reprogrammed cells throughout the initiation and maturation stages. [Stem Cells] Abstract Embryonic Stem Cells Cultured in Microfluidic Chambers Take Control of Their Fate by Producing Endogenous Signals Including LIF Scientists describe the behavior of mouse embryonic stem cells (mESCs) inside microfluidic chambers (microchambers) operated under conditions of minimal perfusion. mESCs inside microchambers formed colonies and expressed markers of pluripotency in the absence of feeders or pluripotency-inducing signals such as leukemia inhibitory factor (LIF), while mESCs in standard cultureware differentiated rapidly. [Stem Cells] Abstract EIF2S3Y Suppresses the Pluripotency State and Promotes the Proliferation of Mouse Embryonic Stem Cells Investigators utilized embryonic stem (ES)-derived Eukaryotic translation initiation factor 2, subunit 3, and structural gene Y-linked (EIF2S3Y)-overexpressing cells and found that EIF2S3Y down-regulated the pluripotency state of ES cells, which might be explained by decreased histone methylation levels because of reduced levels of ten-eleven translocation 1. [Oncotarget] Full Article Chicken Embryonic Stem Cells and Primordial Germ Cells Display Different Heterochromatic Histone Marks than Their Mammalian Counterparts Researchers compared the abundance and nuclear distribution of the main histone post-translational modifications, 5-methylcytosine and 5-hydroxymethylcytosine in chicken embryonic stem cells (ESCs), primordial germ cells and blastodermal cells with differentiated chicken ESCs and embryonic fibroblasts. [Epigenetics Chromatin] Full Article Protein Regulation of Induced Pluripotent Stem Cells by Transplanting in a Huntington’s Animal Model Investigators aimed to determine the functional recovery and protein regulation by transplanted induced pluripotent stem cells in a rat model of Huntington’s disease. At eight weeks after induced pluripotent stem cell transplantation, motor function was improved in comparison with the quinolinic acid-treated rats. [Neuropathol Appl Neurobiol] Abstract Robust Method for TALEN-Edited Correction of pF508del in Patient-Specific Induced Pluripotent Stem Cells Scientists present an efficient method for seamless correction of pF508del mutation in patient-specific induced pluripotent stem cells by gene edited homologous recombination. [Stem Cell Res Ther] Full Article Nitric Oxide Prevents Mouse Embryonic Stem Cell Differentiation through Regulation of Gene Expression, Cell Signaling and Control of Cell Proliferation Researchers sought to demonstrate that nitric oxide positively regulates the pluripotency transcriptional core, enforcing changes in the chromatin structure, in addition to regulating cell proliferation and signaling pathways with key roles in stemness. [J Cell Biochem] Abstract Modeling Xeroderma Pigmentosum Associated Neurological Pathologies with Patients-Derived iPSCs Investigators generated patient-specific induced pluripotent stem cells (iPSCs) harboring mutations in five different xeroderma pigmentosum genes including XPA, XPB, XPC, XPG, and XPV. [Protein Cell] Abstract | Full Article Pleurotus eryngii Polysaccharide Promotes Pluripotent Reprogramming via Facilitating Epigenetic Modification Cationized P. eryngii polysaccharide, hybridized with calcium phosphate (CP), was used to codeliver plasmids for generating induced pluripotent stem cells. The results revealed that the hybrid nanoparticles could significantly enhance the process and efficiency of reprogramming compared with the CP nanoparticles. [J Agric Food Chem] Abstract | Graphical Abstract |