The Acetyllysine Reader BRD3R Promotes Human Nuclear Reprogramming and Regulates Mitosis Scientists identified an isoform of human bromodomain-containing 3 (BRD3), BRD3R (BRD3 with reprogramming activity), as a reprogramming factor. BRD3R positively regulated mitosis during reprogramming, upregulated a large set of mitotic genes at early stages of reprogramming, and associated with mitotic chromatin. [Nat Commun] Full Article | Press Release SOX2 O-GlcNAcylation Alters Its Protein-Protein Interactions and Genomic Occupancy to Modulate Gene Expression in Pluripotent Cells Investigators showed SOX2 is O-GlcNAc modified in its transactivation domain during reprogramming and in mouse embryonic stem cells. [eLife] Abstract | Download Full Article YAP Induces Human Naive Pluripotency Researchers report that the Hippo pathway effector YAP is nuclearly localized in the inner cell mass of human blastocysts. Overexpression of YAP in human embryonic stem cells and induced pluripotent stem cells (PSCs) promoted the generation of naive PSCs. [Cell Rep] Full Article | Graphical Abstract A Systematic Method to Identify Modulation of Transcriptional Regulation via Chromatin Activity Reveals Regulatory Network during mESC Differentiation Investigators applied modulation of transcriptional regulation via chromatin activity to systematically explore the interplay of chromatin regulators, TFs, and target genes in mouse embryonic stem cells (mESCs). [Sci Rep] Full Article Insulin Resistance in Human iPS Cells Reduces Mitochondrial Size and Function To define the cause-effect relationship between insulin resistance and mitochondrial dysfunction, the authors compared mitochondrial metabolism in induced pluripotent stem cells (iPSC) from five healthy individuals and four patients with genetic insulin resistance due to insulin receptor mutations. Insulin-resistant iPSCs had increased mitochondrial number and decreased mitochondrial size. [Sci Rep] Full Article Naive Pluripotent Stem Cells Derived Directly from Isolated Cells of the Human Inner Cell Mass The authors showed that in these specific conditions individual inner cell mass cells grow into colonies that may then be expanded over multiple passages while retaining a diploid karyotype and naive properties. [Stem Cell Reports] Full Article | Graphical Abstract | Press Release A Serial shRNA Screen for Roadblocks to Reprogramming Identifies the Protein Modifier SUMO2 Researchers performed an unbiased serial shRNA enrichment screen to identify potent repressors of somatic cell reprogramming into induced pluripotent stem cells (iPSCs). This effort uncovered the protein modifier SUMO2 as one of the strongest roadblocks to iPSC formation. [Stem Cell Reports] Abstract | Graphical Abstract Donor Dependent Variations in Hematopoietic Differentiation among Embryonic and Induced Pluripotent Stem Cell Lines Investigators compared hematopoietic differentiation potentials from embryonic stem (ES) cells and induced pluripotent stem (iPS) cell lines originated from various donors and derived them using integrative and non-integrative vectors. Significant differences in differentiation toward hematopoietic lineage were observed among ES and iPS cells. [PLoS One] Full Article Multiple Mechanisms Determine the Sensitivity of Human-Induced Pluripotent Stem Cells to the Inducible Caspase-9 Safety Switch Researchers found that inducible caspase-9 (iC9)-resistant induced pluripotent stem cells have significant heterogeneity in terms of their escape mechanisms from caspase-dependent apoptosis including reduced expression of iC9 by promoter silencing and overexpression of BCL2. [Mol Ther Methods Clin Dev] Full Article |