Divergent LncRNAs Regulate Gene Expression and Lineage Differentiation in Pluripotent Cells In pluripotent cells, divergent lncRNAs regulated the transcription of nearby genes. As an example, the divergent lncRNA Evx1as promoted transcription of its neighbor gene, EVX1, and regulates mesendodermal differentiation. [Cell Stem Cell] Abstract | Graphical Abstact MLL1 Inhibition Reprograms Epiblast Stem Cells to Naive Pluripotency Investigators showed that blocking histone H3K4 methyltransferase MLL1 activity with the small-molecule inhibitor MM-401 reprograms mouse epiblast stem cells to naive pluripotency. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Spatial Transcriptome for the Molecular Annotation of Lineage Fates and Cell Identity in Mid-gastrula Mouse Embryo Researchers performed a high-resolution RNA sequencing analysis on single mid-gastrulation mouse embryos to collate a spatial transcriptome that correlated with the regionalization of cell fates in the embryo. [Dev Cell] Abstract | Graphical Abstract Sp5 and Sp8 Recruit β-Catenin and Tcf1-Lef1 to Select Enhancers to Activate Wnt Target Gene Transcription Investigators showed in mouse embryos and differentiating embryonic stem cells that Sp5/8 are gene-specific transcriptional coactivators in the Wnt/β-catenin pathway. [Proc Natl Acad Sci USA] Abstract Rbm24 Regulates Alternative Splicing Switch in ESC Cardiac Lineage Differentiation Researchers identified RNA binding motif protein 24 (Rbm24) as a key splicing regulator that plays an essential role in controlling post-transcriptional networks during embryonic stem cell transition into cardiac differentiation. [Stem Cells] Abstract Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells The authors generated induced pluripotent stem cells (iPSC) from two familial amyotrophic lateral sclerosis patients with a missense mutation in the fused-in sarcoma (FUS) gene carrying the heterozygous FUS H517D mutation, and isogenic iPSCs with the homozygous FUS H517D mutation by genome editing technology. [Stem Cell Rep] Full Article | Graphical Abstract Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells Investigators attempted to elucidate when, under what circumstances, Zscan4 is activated in embryonic stem cells. Using live cell imaging, they monitored the activity of Zscan4 together with the pluripotency marker Rex1. [Stem Cell Reports] Full Article | Grapgical Abstract Porcine Pluripotent Stem Cells Derived from IVF Embryos Contribute to Chimeric Development In Vivo Scientists derived a porcine pluripotent stem cell (pPSCs) line from day 5.5 blastocysts in a newly developed culture system based on MXV medium and a 5% oxygen atmosphere. The pPSCs had been passaged more than 75 times over two years, and the morphology of the colony was similar to that of human embryonic stem cells. [PLoS One] Full Article Human Embryonic Stem Cell Lines with Lesions in FOXP3 and NF1 Investigators obtained preimplantation genetic diagnosed blastocyst staged embryos with genetic mutations that cause human disorders and derived human embryonic stem cells from these embryos. [PLoS One] Full Article |