High-Efficiency Cellular Reprogramming with Microfluidics Scientists report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells can be dramatically improved in a microfluidic environment. [Nat Methods] Abstract Interactions between Pluripotency Factors Specify cis-Regulation in Embryonic Stem Cells The authors investigated how interactions between pluripotency transcription factors (TFs) affect cis-regulation. They created hundreds of synthetic cis-regulatory elements comprised of combinations of binding sites for pluripotency TFs and measured their expression in mouse embryonic stem cells. [Genome Res] Abstract Epigenetic Profiles Signify Cell Fate Plasticity in Unipotent Spermatogonial Stem and Progenitor Cells Investigators found in spermatogonial stem and progenitor cells that promoters essential to maintenance and differentiation of embryonic stem cells (ESCs) are enriched with histone H3-lysine4 and -lysine 27 trimethylations. These bivalent modifications are maintained at most somatic promoters after conversion, bestowing multipotent adult spermatogonial-derived stem cells an ESC-like promoter chromatin. [Nat Commun] Full Article A Novel Lineage Restricted, Pericyte-Like Cell Line Isolated from Human Embryonic Stem Cells Researchers developed a method comparable to GMP quality protocols for deriving self-renewing perivascular progenitors from the human embryonic stem cell, line ESI-017. [Sci Rep] Full Article Culture Conditions Have an Impact on the Maturation of Traceable, Transplantable Mouse Embryonic Stem Cell-Derived Otic Progenitor Cells An embryonic stem cell line derived from an Atoh1-green fluorescent protein transgenic mouse was used to track the generation of otic progenitors, initial hair cells and to compare these two differentiation systems. [J Tissue Eng Regen Med] Abstract Assessment of Developmental Cardiotoxic Effects of Some Commonly Used Phytochemicals in Mouse Embryonic D3 Stem Cell Differentiation and Chick Embryonic Cardiomyocyte Micromass Culture Models Researchers showed that eugenol demonstrated effects on cell activity and ROS production, as well as decreasing the contractile activity and protein content at high concentrations in both systems. Additionally, α-bisabolol at high concentrations decreased the contractile activity and cell activity and in the stem cell system induced ROS production and decreased protein content. [Reprod Toxicol] Abstract | Graphical Abstract Forced Expression of Hnf1b/Foxa3 Promotes Hepatic Fate of Embryonic Stem Cells Investigators hypothesized that lentivirus-mediated inducible expression of hepatic lineage transcription factors could enhance mouse embryonic stem cells to hepatocyte-like cells. [Biochem Biophys Res Commun] Abstract Generation of Human Embryonic Stem Cell Line Expressing zsGreen in Cholinergic Neurons Using CRISPR/Cas9 System Investigators showed that the choline acetyltransferase-zsGreen human embryonic stem cell reporter cell lines retain the features of undifferentiated human embryonic stem cells. [Biochem Biophys Res Commun] Abstract |