Local Genome Topology Can Exhibit an Incompletely Rewired 3D-Folding State during Somatic Cell Reprogramming The authors integrated fine-resolution architecture maps with epigenetic marks and gene expression in embryonic stem cells, neural progenitor cells (NPCs), and NPC-derived induced pluripotent stem cells. They found that most pluripotency genes reconnect to target enhancers during reprogramming. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Tumor Resistance in Induced Pluripotent Stem Cells Derived from Naked Mole-Rats Scientists generated naked mole-rat (NMR)-induced pluripotent stem cells (NMR-iPSCs) and found that NMR-iPSCs do not exhibit teratoma-forming tumorigenicity due to the species-specific activation of tumor-suppressor alternative reading frame and a disruption mutation of the oncogene ES cell-expressed Ras. [Nat Commun] Full Article RISC-Mediated Control of Selected Chromatin Regulators Stabilizes Ground State Pluripotency of Mouse Embryonic Stem Cells Investigators performed an integrated analysis of RNA and proteins at the transition between naïve embryonic stem cells and cells primed to differentiate. During this transition, mRNAs coding for chromatin regulators are specifically released from translational inhibition mediated by RNA-induced silencing complex (RISC). [Genome Biol] Full Article Comparison of Syncytiotrophoblast Generated from Human Embryonic Stem Cells and from Term Placentas Scientists proposed that the syncytiotrophoblast generated from human embryonic stem cells represents the primitive syncytium encountered in early pregnancy soon after the human trophoblast invades into the uterine wall. [Proc Natl Acad Sci USA] Abstract | Full Article A Biomimetic Synthetic Feeder Layer Supports the Proliferation and Self-Renewal of Mouse Embryonic Stem Cells On a biomimetic synthetic feeder layer (BSFL) mouse embryonic stem cells adhered and formed compact round colonies; similar to on MEF controls but not on flat polyacrylamide. ESCs on BSFLs maintained their pluripotency and self-renewal across passages, formed embryoid bodies and differentiated into progenitors of the three germ layers. [Acta Biomater] Abstract Nanog, Oct4 and Tet1 Interplay in Establishing Pluripotency Scientists proposed a multiple level model where the regulatory network of Oct4, Nanog and Tet1 includes positive feedback loops involving DNA-demethylation around the promoters of Oct4 and Tet1. [Sci Rep] Full Article A Gonogenic Stimulated Transition of Mouse Embryonic Stem Cells with Enhanced Control of Diverse Differentiation Pathways The authors discovered that a cellular state with some molecular markers of male gonocyte induction, including a G1/S phase arrest and upregulation of specific genes such as Nanos2, Tdrd1, Ddx4, Zbtb16 and Plk1s1, can be chemically induced in male mouse embryonic stem cells in vitro. [Sci Rep] Full Article Enhanced Generation of Integration-Free iPSCs from Human Adult Peripheral Blood Mononuclear Cells with an Optimal Combination of Episomal Vectors Investigators previously reported the generation of integration-free induced pluripotent stem cells from adult peripheral blood with an improved episomal vector system, which uses the spleen focus-forming virus U3 promoter and an extra factor BCL-XL. Here they showed an ∼100-fold increase in efficiency by optimizing the vector combination. [Stem Cell Reports] Full Article | Graphical Abstract Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells Researchers describe the engineering of bidirectional promoters that drive the expression of precise ratiometric fluorescent miRNA sensors in single mouse embryonic stem cells and their differentiated derivatives. [PLoS One] Full Article |