ESC & iPSC News Volume 11.26 | Jul 6 2016

TOP STORY

Influence of Growth Characteristics of Induced Pluripotent Stem Cells on Their Uptake Efficiency for Layer-by-Layer Microcarriers

The authors investigation focus was set on the evaluation of the capability of layer-by-layer designed microcarriers to serve as a potential drug delivery system to induced pluripotent stem cells, as they offer several appealing advantages. [ACS Nano] Abstract | Graphical Abstract

(Ranked by impact factor of the journal)

A Novel Role for miR-1305 in Regulation of Pluripotency-Differentiation Balance, Cell Cycle and Apoptosis in Human Pluripotent Stem Cells

The authors used a microarray based approach to identify microRNAs that were enriched in human embryonic stem cells when compared to differentiated cells and at the same time showed significant expression changes between different phases of cell cycle. [Stem Cells] Abstract

Neural Patterning of Human Induced Pluripotent Stem Cells in 3-D Cultures for Studying Biomolecule-Directed Differential Cellular Responses

By tuning neural patterning of human induced pluripotent stem cells with small molecules targeting sonic hedgehog signaling, scientists generated different 3-D neuronal cultures that were mainly comprised of either cortical glutamatergic neurons or motor neurons. [Acta Biomater] Abstract

Impact of Feeding Strategies on the Scalable Expansion of Human Pluripotent Stem Cells in Single-Use Stirred Tank Bioreactors

Utilizing single-use instrumented stirred-tank bioreactors, researchers showed that perfusion resulted in a more homogeneous culture environment and enabled superior cell densities of 2.85 × 10⁶ cells per milliliter and 47% higher cell yields compared with conventional repeated batch cultures. [Stem Cells Transl Med] Abstract

Efficient Generation of Corticofugal Projection Neurons from Human Embryonic Stem Cells

Investigators developed a robust, two-step process for generating corticofugal projection neurons from human embryonic stem cells. [Sci Rep] Full Article

Regulation of the DNA Methylation Landscape in Human Somatic Cell Reprogramming by the miR-29 Family

Researchers found that the miR-29 family is an important epigenetic regulator during human somatic cell reprogramming. Their global DNA methylation and hydroxymethylation analysis showed that DNA demethylation is a major event mediated by miR-29a depletion during early reprogramming, and that induced pluripotent stem cells derived from miR-29a depletion are epigenetically closer to embryonic stem cells. [Stem Cell Reports] Full Article | Graphical Abstract

HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells

Investigators demonstrated that hypoxia inducible factor-1alpha (HIF-1α) deficiency significantly altered the quality and quantity of mouse embryonic stem cells -derived cardiomyocytes. [PLoS One] Full Article

Fast and Efficient Transfection of Mouse Embryonic Stem Cells Using Non-Viral Reagents

Researchers performed a side-by-side comparison of commercially available, non-viral transfection reagents with regard to their ability to deliver plasmid DNA and siRNA into adherent and/or trypsinized mouse embryonic stem cells cultured in 2i medium, assessing transfection rates, plasmid gene expression, siRNA mediated knockdown of Oct4 and viability. [Stem Cell Rev] Full Article

Epigenetics Changes Caused by the Fusion of Human Embryonic Stem Cell and Ovarian Cancer Cells

Scientists observed the effect of gene expression and tumorigenicity in hybrid cells of human embryonic stem cells (hESCs) and ovarian cancer cells in vitro and in vivo using a mouse model, and determined its feasibility in reprogramming tumor cells growth and apoptosis, for a potential exploration of the role of hESCs and tumor cells fusion in the management of ovarian cancer. [Biosci Rep] Abstract | Full Article

Single Cell Pluripotency Regulatory Networks

The authors discuss the reconstruction of regulatory networks in individual cells using novel single cell transcriptomics and proteomics, in order to expand our understanding of the molecular basis of pluripotency, including the role of cell-cell variability within embryonic stem cell populations, and ways in which networks may be controlled in order to reliably manipulate cell behavior. [Proteomics] Abstract

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.

$19.5 Million Grant to Bridge Gaps between Medical Research, Health Care in Rhode Island

With a new five-year federal grant, the Rhode Island Center for Clinical Translational Science will strengthen connections between scientific discovery and health around the state. [Brown University] Press Release

Helen F. Graham Cancer Center & Research Institute and The Wistar Institute Partner to Expand Research in Gene Editing

To accelerate breakthrough cancer research in the human genome, the Gene Editing Institute at Christiana Care’s Helen F. Graham Cancer Center & Research Institute has entered into an agreement with The Wistar Institute. [Christiana Care Health System] Press Release

Canada’s Health Funder Agrees to Meet with Researchers Outraged by Peer-Review Changes

Officials at the Canadian Institute of Health Research agreed to a request from Jane Philpott, Canada’s minister of health, to meet with representatives of more than 1000 researchers who have demanded that the agency reverse changes to its grantsmaking process, including a decision to replace face-to-face peer review meetings with an online system. [ScienceInsider] Editorial

U.K. Research Charity Will Self-Publish Results from Its Grantees

Starting sometime this fall, the Wellcome Trust, the charity in London that has become one of the biggest nongovernmental funders of biomedical research, will launch its own open-access online journal. [ScienceInsider] Editorial

NEW 2016 Till & McCulloch Meeting
October 24-26, 2016
Whistler, Canada

Visit our events page to see a complete list of events in the ESC & iPSC community.

NEW Postdoctoral Fellowship(s) – Deciphering the Link Between Adhesion and Pluripotency (The Danish Stem Cell Center)

NEW Postdoctoral Fellow – Pluripotent Stem Cell Technology (University of California, Davis)

Technical Sales Representative (STEMCELL Technologies Inc.)

Research Technologist – Myogenic and Progenitor Cells (STEMCELL Technologies Inc.)

Postdoctoral Position – Bone and Stem Cell Research (University of Connecticut Health)

Postdoctoral Fellow – Cancer Biology and Stem Cell Biology Research (City of Hope)

Postdoctoral Research Fellow – Epigenetics (Institut of Molecular Biology)

Postdoctoral Fellow – Bioscience Research (The University of Tennessee Health Science Center)

Postdoctoral Researcher – Bioinformatics Exploring Early Human Development and Stem Cell Biology (Karolinska Institutet)

Postdoctoral Position – Stem Cell/Genome Editing/Neuroscience (University of California, Davis)

Postdoctoral Fellow (Joslin Diabetes Center)

Scientist – Target Validation (Innate Pharma)

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