ESC & iPSC News Volume 11.35 | Sep 7 2016

TOP STORY

The X to Autosome Expression Ratio in Haploid and Diploid Human Embryonic Stem Cells

Scientists report that the X to autosome expression ratio equals ~1 in haploid human parthenogenetic embryonic stem (pES) cells and ~0.5 in diploidized pES cells, both with one active X chromosome. [Mol Biol Evol] Abstract

(Ranked by impact factor of the journal)

Cripto Is Essential to Capture Mouse Epiblast Stem Cell and Human Embryonic Stem Cell Pluripotency

Investigators demonstrated that Cripto sustains mouse embryonic stem cell (ESC) self-renewal by modulating Wnt/β-catenin, whereas it maintains mouse epiblast stem cell and human ESC pluripotency through Nodal/Smad2. [Nat Commun] Full Article

Generation of Human Muscle Fibers and Satellite-Like Cells from Human Pluripotent Stem Cells In Vitro

Scientists report an optimized serum-free differentiation protocol to efficiently produce striated, millimeter-long muscle fibers together with satellite-like cells from human pluripotent stem cells in vitro. [Nat Protoc] Abstract

A Panel of Embryonic Stem Cell Lines Reveals the Variety and Dynamic of Pluripotent States in Rabbits

The authors explored the capacity of the rabbit inner cell mass to give rise to embryonic stem cell (ESC) lines using conditions similar to those utilized to generate naive ESCs in mice. [Stem Cell Reports] Full Article

Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells

Investigators introduce a donor-free paired gRNA-guided CRISPR/Cas9 knockout strategy for efficient and rapid gene ablation in human pluripotent stem cells. [Stem Cell Reports] Full Article | Graphical Abstract

The Combination of CRISPR/Cas9 and iPSC Technologies in the Gene Therapy of Human β-Thalassemia in Mice

Researchers reported that correction of β-thalassemia mutations in patient-specific induced pluripotent stem cells (iPSCs) using the CRISPR/Cas9 tool promotes hematopoietic differentiation in vivo. [Sci Rep] Full Article

Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes

Using a chemically defined albumin-free medium, scientists identified heparin as a novel factor that significantly promotes cardiomyocyte differentiation efficiency, and developed an efficient method to differentiate human pluripotent stem cells into cardiomyocytes. [Stem Cells Transl Med] Abstract | Full Article

Hepatoprotective Effect of Exosomes from Human-Induced Pluripotent Stem Cell–Derived Mesenchymal Stromal Cells against Hepatic Ischemia-Reperfusion Injury in Rats

The authors evaluated the effect of exosomes produced by human-induced pluripotent stem cell–derived mesenchymal stromal cells on hepatic ischemia-reperfusion injury. [Cytotherapy] Abstract

Downregulation of IGF-1 Receptor Occurs after Hepatic Lineage Commitment during Hepatocyte Differentiation from Human Embryonic Stem Cells

The expression levels of insulin-like growth factor (IGF)-1 receptor and major downstream signaling proteins during differentiation from human embryonic stem cells to mature hepatocytes were investigated. [Biochem Biophys Res Commun] Full Article

Modulation of Human Multipotent and Pluripotent Stem Cells Using Surface Nanotopographies and Surface-Immobilized Bioactive Signals: A Review

The authors provide an overview of the current state-of-the-art in the design of complex material surfaces aimed at being the next generation of tools tailored for applications in cell culture and regenerative medicine. [Acta Biomater] Abstract

Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field.

Objective Capital Partners Adds CardioCell to Expanding Portfolio of Life Science Companies

Objective Capital Partners announced that it has recently been retained by CardioCell LLC to help manage its growth needs. [Objective Capital Partners LLC] Press Release

Paralyzed Man Regains Use of Arms and Hands after Experimental Stem Cell Therapy at Keck Hospital of USC

Keck Medical Center of USC announced that a team of doctors became the first in California to inject an experimental treatment made from stem cells, AST-OPC1, into the damaged cervical spine of a recently paralyzed 21-year-old man as part of a multi-center clinical trial. [Keck Medicine of USC (PR Newswire Association LLC)] Press Release

In Wake of Attacks, German Science Bodies Fend for Animal Research

Germany’s major research institutes have teamed up to publicly defend “responsible” animal testing as a necessary part of biomedical science. [Science News] Editorial

Boom in Unproven Cell Therapies Intensifies Regulatory Debate

Controversial US guidelines attempt to rein in rogue stem-cell clinics. [Nature News] Editorial

NEW Advances in Cell Engineering, Imaging, and Screenings
November 17-18, 2016
Leuven, Belgium

Visit our events page to see a complete list of events in the community.

NEW Research Associate – Stem Cell Research (University of Miami)

Technical Sales Representative (STEMCELL Technologies Inc.)

Research Technologist – Cancer Research (Penn State College of Medicine)

Postdoctoral Fellow Position – Stem Cell Biology & Immunology (Toronto General Research Institute & McEwen Centre for Regenerative Medicine)

Group Leader – Stem Cells and Aging (University of Queensland)

Postdoctoral Position – Metabolic Regulation of Embryonic and Stem Cell Biology (Joslin Diabetes Center)

PhD Studentship – Fibrosis (National University of Ireland, Galway)

Assistant Professor – Developmental Biology (Washington University School of Medicine)

Core Staff Scientist – Cancer Research (Moffitt Cancer Center)

Postdoctoral Position – Regenerative Medicine (University of California, San Francisco)

Postdoctoral Position – Cardiovascular Stem Cell Biology (Yale University)

Industrial Research Manager – Regenerative Medicine and Tissue Engineering (KU Leuven)

Postdoctoral Fellow – Pluripotent Stem Cell Technology (University of California, Davis)

Postdoctoral Position – Bone and Stem Cell Research (University of Connecticut Health)

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