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Pulmonary Cell News| ESC & iPSC News 13.05 February 7, 2018 | |
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TOP STORYIn Vitro Modeling of Human Germ Cell Development Using Pluripotent Stem Cells The authors developed a feeder- and xeno-free culture protocol that efficiently human iPSCs into PLZF+/GPR125+/CD90+ spermatogonium-like cells (SLCs). These SLCs were enriched with key genes in germ cell development such as MVH, DAZL, GFRα1, NANOS3, and DMRT1. [Stem Cell Reports] Full Article | |
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PUBLICATIONS(Ranked by impact factor of the journal)Researchers showed that low levels of Wnt stimulation via ectopic expression of Wnt1 or administration of GSK-3 inhibitor CHIR99021 significantly increases PSC differentiation into neurons, cardiomyocytes and early endodermal intermediates. Their data indicated that enhanced differentiation outcomes are not mediated through activation of traditional Wnt target genes but by β-catenin’s secondary role as a binding partner of membrane bound cadherins ultimately leading to the activation of developmental genes. [Stem Cells] Abstract Investigators generated mouse chemical iPSCs (C-iPSCs) and OSKM-iPSCs (4F-iPSCs), and further compared the genome-wide DNA methylomes of C-iPSCs, 4F-iPSCs, and mouse ESCs (mESCs) that were maintained in 2i and LIF. Three pluripotent stem cells tended to be low methylated overall, however, DNA methylations in some specific regions (such as retrotransposons) were cell type-specific. [Cell Death Dis] Full Article Generation of a Rod-Specific NRL Reporter Line in Human Pluripotent Stem Cells The authors created the first human rod reporter line using CRISPR/Cas9 genome editing to replace one allele of the Neural Retina Leucine zipper (NRL) gene with an eGFP transgene in the WA09 human ESC (hESC) line. The NRL+/eGFP hESC line robustly and exclusively labeled the entirety of rods throughout differentiation, eventually revealing highly mature structural features. [Sci Rep] Full Article Using CRISPR/Cas9 delivered as a RNA modality in conjunction with a lipid specifically formulated for large RNA molecules, scientists demonstrated that homology directed repair rates between 20–40% can be achieved in iPSCs. [Sci Rep] Full Article Researchers aimed to develop a 3D hematopoietic differentiation approach that serves as recapitulation of embryonic development in vitro to a degree of complexity not achievable in a 2D culture system. Colony-forming cells assay results suggested mPSCs could differentiate into multipotential progenitor cells and 3D induction system derived hematopoietic colonies owned potential of differentiating into lymphocyte cells. [J Cell Physiol] Abstract Effects of Various Culture Conditions on Pluripotent Stem Cell Derivation from Chick Embryos Investigators determined the effects of PSC culture media, including serum-based and serum-free media as well as various feeder layers, growth factors, and small molecules on derivation and maintenance of avian embryonic derived-PSCs. [J Cell Biochem] Abstract Generation of Isthmic Organizer-Like Cells from Human Embryonic Stem Cells Scientists induced the production of isthmic organizer (IsO)-like cells capable of secreting fibroblast growth factor 8 and WNT1 from human ESCs. The precise modulation of canonical Wnt signaling was achieved in the presence of the small molecule CHIR99021 during the neural induction of human ESCs, resulting in the differentiation of these cells into IsO-like cells having a midbrain-hindbrain border fate in a manner that recapitulated their developmental course in vivo. [Mol Cells] Abstract Researchers defined the roles of Mll4/COMPASS (COMplex of Proteins ASsociated with Set1) and its catalytic activity in the maintenance and exit of ground-state pluripotency in murine ESCs. Mll4 was required for ESCs to exit the naive pluripotent state; however, its intrinsic catalytic activity is dispensable for this process. [Sci Adv] Full Article | |
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REVIEWSMetabolism in Pluripotent Stem Cells and Early Mammalian Development The authors discuss metabolism as a potential driver of earliest cell fate through its influence on the epigenome and gene expression in embryos and their in vitro surrogate pluripotent stem cells. [Cell Metab] Abstract Deriving iPS cells from patients suffering from familial cancers provides a valuable tool for understanding the mechanisms underlying pediatric cancer onset and progression since they require less mutation recurrence than adult cancers to develop. [Cell Death Discov] Full Article Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field. | |
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INDUSTRY NEWSUS Biomed Consortium Awards US$300k Grant to Progress Advanced Regenerative Tissue Product The Medical Technology Enterprise Consortium has awarded a US$300,000 research grant to regenerative tissue developer, Upside Biotechnologies. New Zealand-based Upside is developing an advanced, world-class skin replacement treatment for patients suffering major burns. [Upside Biotechnologies Ltd.] Press Release UVM Joins High-Profile Regenerative Medicine Consortium The University of Vermont (UVM) has joined the Advanced Regenerative Manufacturing Institute (ARMI) through its BioFabUSA program. ARMI is a non-profit, federally sponsored consortium dedicated to making the large-scale manufacture of engineered tissues and tissue-related technologies practical, to benefit existing industries and grow new ones. [The University of Vermont] Press Release | |
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POLICY NEWSPeace is a dangerous cause to fight for in Turkey right now. In the latest blow to academics, 11 members of the Turkish Medical Association, including its president, RaÅŸit Tükel, were arrested in early-morning raids. [Nature News] Editorial Japanese Researchers Say Science-Budget Hike Isn’t Enough Japan will sharply increase funding for science and technology in 2018 after years of stagnant support for the sector. But some scientists and policy experts worry the boost is too little to make up for more than a decade of lackluster science funding. [Nature News] Editorial Kid Co-Authors in South Korea Spur Government Probe The South Korean government is expanding an investigation into researchers who named their children as co-authors on papers. The extended probe comes after a government report identified 82 academic papers on which authors had named their children or relatives — many of them in middle or high school — as co-authors on the publications. [Nature News] Editorial
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EVENTSNEW The New York Stem Cell Foundation (NYSCF) Conference 2018 Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Postdoctoral Position – Metabolic Regulation of Embryos and Stem Cells (Joslin Diabetes Center) NEW Associate Scientific Editor – Cell Reports/Cell Stem Cell (Elsevier) NEW Postdoctoral Research Assistant – Stem Cell Biology (University of Oxford) Research Assistant – Stem Cell Modeling (NUI Galway) Postdoctoral Researcher – Cell-Based Cardiac Regeneration (Karolinska Institutet) Postdoctoral Position – Pancreas Development and Stem Cells (Helmholtz Zentrum München) Postdoctoral Fellowship – Neurodegeneration (Lund University) Research Scientist – Stem Cell Research (Evotec AG) Postdoctoral Research Fellows – Bioengineering and Nanotechnology (The University of Queensland) Postdoctoral Position – Regenerative Medicine (University of Pennsylvania) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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