ESC & iPSC News 15.08 March 4, 2020 | |
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TOP STORYLamin B2 Levels Regulate Polyploidization of Cardiomyocyte Nuclei and Myocardial Regeneration Inactivating lamin B2 (LMNB2) in human iPS cell-derived cardiomyocytes reduced karyokinesis and increased formation of polyploid nuclei. In primary cardiomyocytes from human infants with heart disease, modifying LMNB2 expression correspondingly altered metaphase progression and ploidy of daughter nuclei. [Dev Cell] Full Article | Graphical Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)Robustness of Catalytically-Dead Cas9 Activators in Human Pluripotent and Mesenchymal Stem Cells Researchers compared the ability of the first-generation catalytically-dead Cas9 (dCas9)-VP64 activator and the second-generation systems, dCas9-SAM and dCas9-SunTag, to induce gene expression in human PSCs and human mesenchymal stem cells. [Mol Ther Nucleic Acids] Abstract | Full Article The authors found that disrupting the expression of HIF-1α reduced self-renewal and pluripotency of human iPSCs (hiPSCs). HIF-1α-knockdown led to lower mitochondrial membrane potential and higher reactive oxygen species production in hiPSCs. [FASEB J] Full Article Investigators used a modified protocol to differentiate iPSCs from Huntington’s disease (HD) patients and unaffected controls into neuronal cultures enriched for medium spiny neurons, the cell type most affected in HD. They performed single-cell and bulk transcriptomic and epigenomic analyses and demonstrated that a persistent cyclin D1+ neural stem cell population was observed selectively in adult-onset HD iPSCs during differentiation. [Stem Cell Reports] Full Article | Graphical Abstract The authors showed that the N-terminal proline remnant of the 2A peptide, alone or in combination with leucine, introduced during polycistronic cloning, destabilized KLF4 resulting in increased protein degradation, which hindered reprogramming. [Stem Cell Reports] Full Article | Graphical Abstract Generation of Human Androgenetic Induced Pluripotent Stem Cells Researchers generated human androgenetic iPSCs (AgHiPSCs) from primary androgenetic fibroblasts derived from complete hydatidiform moles. To investigate the pluripotency state of AgHiPSCs, they analyzed their cellular and molecular characteristics. [Sci Rep] Full Article The iPSC-derived EGFP-positive cells exhibited elevated expression of tendon-specific genes, including Scx, Mohawk, Tenomodulin, and Fibromodulin, indicating that they had tenocyte-like properties. [Sci Rep] Full Article The Stemina devTOX quickPredict platform is a human pluripotent stem cell-based assay that predicts the developmental toxicity potential based on changes in cellular metabolism following chemical exposure. Using this assay, scientists screened 1065 ToxCast Phase I and II chemicals in single-concentration or concentration-response for the targeted biomarker. [Toxicol Sci] Abstract Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors The authors generated minicircle vectors containing bone morphogenetic protein 2 and transforming growth factor beta 3 and delivered them to mesenchymal stem cell-like, human iPSC-derived outgrowth (OG) cells. Cell pellets generated using minicircle-transfected OG cells successfully differentiated into the chondrogenic lineage. The implanted minicircle-based chondrogenic pellets recovered the osteochondral defects in rat models. [Cells] Abstract | Full Article Download Subscribe to one of our other 19 science newsletters such as Cell Therapy News & Mesenchymal Cell News. | |
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REVIEWSScientists summarize the function of Yes-associated protein (YAP) and PDZ binding motif (TAZ) and focus on the regulation of YAP/TAZ in self-renewal and differentiation of human ESCs. [Cell Reprogram] Abstract Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field. | |
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INDUSTRY NEWSNanoSurface Biomedical Awarded $1.9M in Funding from NIH NanoSurface Biomedical received $1.9 million in new funding in 2019 from the National Institutes of Health (NIH) to support accelerated development of NanoSurface’s innovative human iPSC-based platforms for drug discovery and safety screening. [NanoSurface Biomedical] Press Release MD Anderson Receives More than $20 Million in CPRIT Funding The University of Texas MD Anderson Cancer Center was awarded more than $20 million from the Cancer Prevention and Research Institute of Texas (CPRIT) to support research, clinical translation, prevention and recruitment efforts. In total, MD Anderson received 28% of the $78 million in awards announced by CPRIT. [MD Anderson Cancer Center] Press Release | |
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POLICY NEWSScientists Grapple with US Restrictions on Fetal Tissue Research The Trump administration’s changes to policy involving material donated from abortions have led scientists to adjust their research projects or seek alternative sources of funding. [The Scientist] Editorial China Bans Cash Rewards for Publishing Papers Chinese institutions have been told to stop paying researchers bonuses for publishing in journals, as part of a new national policy to cut perverse incentives that encourage scientists to publish lots of papers rather than focus on high-impact work. [Nature News] Editorial
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EVENTSNEW Tissue Engineering and Regenerative Medicine Asia-Pacific (TERMIS-AP) Conference 2020 Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESHead – Automated Stem Cell and Organoid Facility (Fondazione Human Technopole) Postdoctoral Scholarship – Non-Coding RNAs (University of Gothenburg) Postdoctoral Associate – Cerebral Organoids (Weill Cornell Medicine) Postdoctoral Fellowship – Stem Cell Niche Biology (University of California, San Francisco) Postdoctoral Positions – Create ‘Foie Gras’ in Vitro from Stem Cells (KU Leuven) PhD Position – Human Inducible Stem Cells (University of Fribourg) Postdoctoral Positions – Stem Cell Biology (Albert Einstein College of Medicine) Postdoctoral Research Fellow – Endocrinology (Cedars-Sinai Medical Center) Assistant Specialist – Division of Cardiovascular Medicine (University of California, Davis) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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