ESC & iPSC News Volume 15.32 | Aug 26, 2020

May 12, 2022

2020-08-26 | ESC 15.32

ESC & iPSC News by STEMCELL Technologies

Vol. 15.32 – 26 August, 2020

TOP STORY

Direct
DNA Crosslinking with Cap-C Uncovers Transcription-Dependent Chromatin Organization at High Resolution

Researchers applied crosslinking assisted proximity capture (CAP-C) to formaldehyde prefixed mouse ESCs and investigated loop domains and nonloop domains. They showed that CAP-C could be used to detect native chromatin conformation without formaldehyde prefixing.
[Nature Biotechnology]

Full Article

Virtual Poster Presentation: CRISPR-Cas9 Gene Editing of Cerebral Organoids to Model Microcephaly

PUBLICATIONSRanked by the impact factor of the journal

Defects
in mRNA Translation in LRRK2-Mutant hiPSC-Derived Dopaminergic Neurons Lead to Dysregulated Calcium Homeostasis

Scientists define the translational landscape in leucine-rich repeat kinase 2 (LRRK2)-mutant dopaminergic neurons derived from human iPSCs via ribosome profiling.
[Cell Stem Cell]

Abstract Graphical Abstract

Glis1
Facilitates Induction of Pluripotency via an Epigenome–Metabolome–Epigenome Signaling Cascade

Investigators highlighted Glis1 as a powerful reprogramming factor, and revealed an epigenome–metabolome–epigenome signaling cascade that involved the glycolysis-driven coordination of histone acetylation and lactylation in the context of cell fate determination.
[Nature Metabolism]

Abstract

Inositol Monophosphatase 1 (IMPA1)
Mutation in Intellectual Disability Patients Impairs Neurogenesis but Not Gliogenesis

To understand the role of IMPA1 deficiency in intellectual disability, scientists generated iPSCs from patients and neurotypical controls and differentiated these into hippocampal dentate gyrus-like neurons and astrocytes.
[Molecular Psychiatry]

Abstract

Differentiation
of a Contractile, Ureter-Like Tissue, from Embryonic Stem Cell–Derived Ureteric Bud and Ex Fetu Mesenchyme

Investigators used a published sequence of growth factors and drugs to induce mouse ESCs to differentiate into ureteric bud tissue. They characterized isolated engineered ureteric buds differentiated from ESCs in 3D culture and grafted them into ex fetu mouse kidney rudiments.
[Journal of the American Society of Nephrology]

Abstract

Single-Cell
Transcriptome Profiling Reveals β Cell Maturation in Stem Cell-Derived Islets after Transplantation

The authors provide a comprehensive resource for understanding human islet cell maturation and provides important insights into maturation of human pluripotent stem cells differentiated to insulin-secreting β cells (SC-β cells) and other SC-islet cell types to enable future differentiation strategy improvements.
[Cell Reports]

Full Article Graphical Abstract

β-Catenin
and Associated Proteins Regulate Lineage Differentiation in Ground State Mouse Embryonic Stem Cells

β-Catenin is required to maintain ground state pluripotency in mouse ESCs, but its exact role is controversial. Researchers revealed a Tcf3-independent role of β-catenin in restraining germline and somatic lineage differentiation genes. They showed that β-catenin binds target genes with E2F6 and forms a complex with E2F6 and HMGA2 or E2F6 and HP1γ.
[Stem Cell Reports]

Full Article

Canonical
Wnt Pathway Controls mESC Self-Renewal through Inhibition of Spontaneous Differentiation via β-Catenin/TCF/LEF Functions

Scientists generated a novel β-catenin knockout model in mouse ESCs (mESCs) to delete putatively functional N-terminally truncated isoforms observed in previous knockout models. They showed that aberrant N-terminally truncated isoforms are not functional in mESCs.
[Stem Cell Reports]

Full Article

4-Phenylbutyrate
Ameliorates Apoptotic Neural Cell Death in Down Syndrome by Reducing Protein Aggregates

The authors investigated cellular stress responses in human trisomy 21 and 13 neurons differentiated from patient-derived iPSCs. Their results suggested that aneuploidy-associated stress might be a therapeutic target for the neurodegenerative phenotypes in Down syndrome.
[Scientific Reports]

Full Article

Malignant Transformation Involving CXXC4
Mutations Identified in a Leukemic Progression Model of Severe Congenital Neutropenia

To investigate how the combination of CSF3 therapy and CSF3R and RUNX1 mutations contributes to acute myeloid leukemia (AML) development, researchers made use of mouse models, severe congenital neutropenia (SCN)-derived iPSCs, and SCN and SCN-AML patient samples.
[Cell Reports Medicine]

Full Article Graphical Abstract

Checking your cell quality doesn’t have to be difficult - Genetic screening with the hPSC Genetic Analysis Kit

REVIEWS

Role
of Induced Pluripotent Stem Cells in Lysosomal Storage Diseases

The authors present the disease and treatment concepts gathered using selected lysosomal storage diseases (LSD)-iPSCs, discuss iPSC research limitations, and set future research visions.
[Molecular and Cellular Neuroscience]

Abstract

INDUSTRY AND POLICY NEWS

Cytovia Therapeutics and NYSCF Announce Filing of Provisional Patent for iPSC-Derived NK Cells to Produce Unlimited On-Demand NK and CAR-NK Cells for the Treatment of Cancer

Cytovia Therapeutics, has announced the filing of a provisional patent application with the US Patent & Trademark Office for the differentiation of natural killer (NK) cells from iPSCs. The NYSCF Research Institute is a pioneer and acknowledged leader in stem cell technology, having developed the premier automated robotic platform for reprogramming skin or blood into iPSCs and differentiating them into disease-relevant cell types.
[Cytovia Therapeutics (GlobeNewswire, Inc.)]

Press Release

FEATURED EVENT

Direct
DNA Crosslinking with Cap-C Uncovers Transcription-Dependent Chromatin Organization at High Resolution

Defects
in mRNA Translation in LRRK2-Mutant hiPSC-Derived Dopaminergic Neurons Lead to Dysregulated Calcium Homeostasis

Glis1
Facilitates Induction of Pluripotency via an Epigenome–Metabolome–Epigenome Signaling Cascade

Inositol Monophosphatase 1 (IMPA1)
Mutation in Intellectual Disability Patients Impairs Neurogenesis but Not Gliogenesis

Differentiation
of a Contractile, Ureter-Like Tissue, from Embryonic Stem Cell–Derived Ureteric Bud and Ex Fetu Mesenchyme

Single-Cell
Transcriptome Profiling Reveals β Cell Maturation in Stem Cell-Derived Islets after Transplantation

β-Catenin
and Associated Proteins Regulate Lineage Differentiation in Ground State Mouse Embryonic Stem Cells

Canonical
Wnt Pathway Controls mESC Self-Renewal through Inhibition of Spontaneous Differentiation via β-Catenin/TCF/LEF Functions

4-Phenylbutyrate
Ameliorates Apoptotic Neural Cell Death in Down Syndrome by Reducing Protein Aggregates

Malignant Transformation Involving CXXC4
Mutations Identified in a Leukemic Progression Model of Severe Congenital Neutropenia

Role
of Induced Pluripotent Stem Cells in Lysosomal Storage Diseases

Cytovia Therapeutics and NYSCF Announce Filing of Provisional Patent for iPSC-Derived NK Cells to Produce Unlimited On-Demand NK and CAR-NK Cells for the Treatment of Cancer

Cancer Research & Oncology 2020

> See All Events

Postdoctoral Research Scientist – Amyotrophic Lateral Sclerosis

Postdoctoral
Fellow – Scholarship in Immunogenetics

Tenure-Track Faculty Positions – Stem Cell Biology

Junior Research Group Leader Position – Experimental Oncology and Stem Cell Biology

Postdoctoral
Research Fellow – Retinal Regeneration and Cell Transplantation

Assistant Scientist – Neurological Disease Modeling

> See All Jobs